LOCUS       .                       7060 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        1026..1241
                     /gene="S. cerevisiae URA3"
                     /label="URA3 promoter"
                     /note="color: #ffffff"
     protein_bind    94..110
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
                     /label="lac operator"
     primer_bind     complement(70..86)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /note="color: #a020f0"
                     /label="M13 rev"
     promoter        complement(118..148)
                     /note="promoter for the E. coli lac operon"
                     /note="color: #ffffff"
                     /label="lac promoter"
     misc_feature    1..57
                     /gene=""
                     /note="pUC19 multiple cloning site"
                     /note="color: #99ccff"
                     /label="MCS"
     promoter        complement(6856..6960)
                     /gene="bla"
                     /label="AmpR promoter"
                     /note="color: #ffffff"
     rep_origin      complement(5236..5824)
                     /direction=LEFT
                     /note="high-copy-number colE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /label="ori"
     CDS             1242..2045
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /note="yeast auxotrophic marker, counterselectable with
                     5-fluoroorotic acid (5-FOA)"
                     /note="color: #ff7f50"
                     /product="orotidine-5'-phosphate decarboxylase, required
                     for uracil biosynthesis"
                     /transl_table=1
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN*"
                     /label="URA3"
     CDS             complement(5995..6855)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ccffcc"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     misc_feature    3390..4768
                     /note="S. cerevisiae CEN4 centromere fused to the
                     autonomously replicating sequence ARS1/ARS416"
                     /note="color: #454f59"
                     /label="CEN/ARS"
ORIGIN
        1 gaattcgagc tcggtacccg gggatcctct agagtcgacc tgcaggcatg caagcttggc
       61 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
      121 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
      181 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagtcgac
      241 cgatgccctt gagagccttc aacccagtca gctccttccg gtgggcgcgg ggcatgacta
      301 tcgtcgccgc acttatgact gtcttcttta tcatgcaact cgtaggacag gtgccggcag
      361 cgctctgggt cattttcggc gaggaccgct ttcgctggag cgcgacgatg atcggcctgt
      421 cgcttgcggt attcggaatc ttgcacgccc tcgctcaagc cttcgtcact ggtcccgcca
      481 ccaaacgttt cggcgagaag caggccatta tcgccggcat ggcggccgac gcgctgggct
      541 acgtcttgct ggcgttcgcg acgcgaggct ggatggcctt ccccattatg attcttctcg
      601 cttccggcgg catcgggatg cccgcgttgc aggccatgct gtccaggcag gtagatgacg
      661 accatcaggg acagcttcaa ggatcgctcg cggctcttac cagcctaact tcgatcactg
      721 gaccgctgat cgtcacggcg atttatgccg cctcggcgag cacatggaac gggttggcat
      781 ggattgtagg cgccgcccta taccttgtct gcctccccgc gttgcgtcgc ggtgcatgga
      841 gccgggccac ctcgacctga atggaagccg gcggcacctc gctaacggat tcaccactcc
      901 aagaattgga gccaatcaat tcttgcggag aactgtgaat gcgcaaacca acccttggca
      961 gaacatatcc atcgcgtccg ccatctccag cagccgcacg cggcgcatcg gggggggggt
     1021 ttcaattcaa ttcatcattt tttttttatt cttttttttg atttcggttt ctttgaaatt
     1081 tttttgattc ggtaatctcc gaacagaagg aagaacgaag gaaggagcac agacttagat
     1141 tggtatatat acgcatatgt agtgttgaag aaacatgaaa ttgcccagta ttcttaaccc
     1201 aactgcacag aacaaaaacc tgcaggaaac gaagataaat catgtcgaaa gctacatata
     1261 aggaacgtgc tgctactcat cctagtcctg ttgctgccaa gctatttaat atcatgcacg
     1321 aaaagcaaac aaacttgtgt gcttcattgg atgttcgtac caccaaggaa ttactggagt
     1381 tagttgaagc attaggtccc aaaatttgtt tactaaaaac acatgtggat atcttgactg
     1441 atttttccat ggagggcaca gttaagccgc taaaggcatt atccgccaag tacaattttt
     1501 tactcttcga agacagaaaa tttgctgaca ttggtaatac agtcaaattg cagtactctg
     1561 cgggtgtata cagaatagca gaatgggcag acattacgaa tgcacacggt gtggtgggcc
     1621 caggtattgt tagcggtttg aagcaggcgg cagaagaagt aacaaaggaa cctagaggcc
     1681 ttttgatgtt agcagaattg tcatgcaagg gctccctatc tactggagaa tatactaagg
     1741 gtactgttga cattgcgaag agcgacaaag attttgttat cggctttatt gctcaaagag
     1801 acatgggtgg aagagatgaa ggttacgatt ggttgattat gacacccggt gtgggtttag
     1861 atgacaaggg agacgcattg ggtcaacagt atagaaccgt ggatgatgtg gtctctacag
     1921 gatctgacat tattattgtt ggaagaggac tatttgcaaa gggaagggat gctaaggtag
     1981 agggtgaacg ttacagaaaa gcaggctggg aagcatattt gagaagatgc ggccagcaaa
     2041 actaaaaaac tgtattataa gtaaatgcat gtatactaaa ctcacaaatt agagcttcaa
     2101 tttaattata tcagttatta cccgggaatc tcggtcgtaa tgatttttat aatgacgaaa
     2161 aaaaaaaaat tggaaagaaa accccccccc cgcagcgttg ggtcctggcc acgggtgcgc
     2221 atgatcgtgc tcctgtcgtt gaggacccgg ctaggctggc ggggttgcct tactggttag
     2281 cagaatgaat caccgatacg cgagcgaacg tgaagcgact gctgctgcaa aacgtctgcg
     2341 acctgagcaa caacatgaat ggtcttcggt ttccgtgttt cgtaaagtct ggaaacgcgg
     2401 aagtcagcgc cctgcaccat tatgttccgg atctgcatcg caggatgctg ctggctaccc
     2461 tgtggaacac ctacatctgt attaacgaag cgctggcatt gaccctgagt gatttttctc
     2521 tggtcccgcc gcatccatac cgccagttgt ttaccctcac aacgttccag taaccgggca
     2581 tgttcatcat cagtaacccg tatcgtgagc atcctctctc gtttcatcgg tatcattacc
     2641 cccatgaaca gaaattcccc cttacacgga ggcatcaagt gaccaaacag gaaaaaaccg
     2701 cccttaacat ggcccgcttt atcagaagcc agacattaac gcttctggag aaactcaacg
     2761 agctggacgc ggatgaacag gcagacatct gtgaatcgct tcacgaccac gctgatgagc
     2821 tttaccgcag gtgggccatt ctcatgaaga atatcttgaa tttattgtca tattactagt
     2881 tggtgtggaa gtccatatat cggtgatcaa tatagtggtt gacatgctgg ctagtcaaca
     2941 ttgagccttt tgatcatgca aatatattac ggtattttac aatcaaatat caaacttaac
     3001 tattgacttt ataacttatt taggtggtaa cattcttata aaaaagaaaa aaattactgc
     3061 aaaacagtac tagcttttaa cttgtatcct aggttatcta tgctgtctca ccatagagaa
     3121 tattacctat ttcagaatgt atgtccatga ttcgccgggt aaatacatat aatacacaaa
     3181 tctggcttaa taaagtctat aatatatctc ataaagaagt gctaaattgg ctagtgctat
     3241 atatttttaa gaaaatttct tttgactaag tccatatcga ctttgtaaaa gttcacttta
     3301 gcatacatat attacacgag ccagaaattg taacttttgc ctaaaatcac aaattgcaaa
     3361 atttaattgc ttgcaaaagg tcacatgctt ataatcaact tttttaaaaa tttaaaatac
     3421 ttttttattt tttattttta aacataaatg aaataattta tttattgttt atgattaccg
     3481 aaacataaaa cctgctcaag aaaaagaaac tgttttgtcc ttggaaaaaa agcactacct
     3541 aggagcggcc aaaatgccga ggctttcata gcttaaactc tttacagaaa ataggcatta
     3601 tagatcagtt cgagttttct tattcttcct tccggtttta tcgtcacagt tttacagtaa
     3661 ataagtatca cctcttagag ttaactatga gataagcaag tatcatctca tttcatttac
     3721 ctgaagtcga gtaaacagaa aatccaattg ttgatgaacc tcaatgactt agaactatct
     3781 atcggcagat catataaaga ggatttaggt actcgagaac gctccgtaca tgcagctaaa
     3841 cctccaagca tctctaactt gggtagtgct ttctccacca ttttctgtgc ttgctccttc
     3901 gtggcaagtc cagcccataa tgcccagaat gtagttgcgg attcgtatga cgttctgtgc
     3961 ttgatttttg tgttgtagtc aaagaaaaac cccgactcgt catcccacat atatttggta
     4021 attgatgagg caacgctaat tatcaacata tagattgtta tctatctgca tgaacacgaa
     4081 atctttactt gacgacttga ggctgatggt gtttatgcaa agaaaccact gtgtttaata
     4141 tgtgtcactg tttgatatta ctgtcagcgt agaagataat agtaaaagcg gttaataagt
     4201 gtatttgaga taagtgtgat aaagttttta cagcgaaaag acgataaata caagaaaatg
     4261 attacgagga tacggagaga ggtatgtaca tgtgtattta tatactaagc tgccggcggt
     4321 tgtttgcaag accgagaaaa ggctagcaag aatcgggtca ttgtagcgta tgcgcctgtg
     4381 aacattctct tcaacaagtt tgattccatt gcggtgaaat ggtaaaagtc aaccccctgc
     4441 gatgtatatt ttcctgtaca atcaatcaaa aagccaaatg atttagcatt atctttacat
     4501 cttgttattt tacagatttt atgtttagat cttttatgct tgcttttcaa aaggcctgca
     4561 ggcaagtgca caaacaatac ttaaataaat actactcagt aataacctat ttcttagcat
     4621 ttttgacgaa atttgctatt ttgttagagt cttttacacc atttgtctcc acacctccgc
     4681 ttacatcaac accaataacg ccatttaatc taagcgcatc accaacattt tctggcgtca
     4741 gtccaccagc taacataaaa tgtaagctct gcctcgcgcg tttcggtgat gacggtgaaa
     4801 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
     4861 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga
     4921 cccagtcacg tagcgatagc ggagtgtata ctggcttaac tatgcggcat cagagcagat
     4981 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata
     5041 ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     5101 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     5161 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     5221 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     5281 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     5341 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     5401 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     5461 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     5521 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     5581 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     5641 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     5701 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     5761 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     5821 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     5881 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     5941 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     6001 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     6061 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     6121 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     6181 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     6241 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     6301 tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     6361 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     6421 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     6481 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     6541 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     6601 cccggcgtca acacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     6661 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     6721 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     6781 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     6841 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     6901 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     6961 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
     7021 ctataaaaat aggcgtatca cgaggccctt tcgtcttcaa
//
