LOCUS       .                       9822 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             9478..9495
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /transl_table=1
                     /translation="HHHHHH"
                     /label="6xHis"
                     /note="color: #cc99b2"
     promoter        5230..5248
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff"
                     /label="T7 promoter"
     CDS             9445..9465
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /note="color: #993366"
                     /product="nuclear localization signal of SV40 large T
                     antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     RBS             5288..5310
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
                     /note="color: #a6acb3"
                     /label="RBS"
     protein_bind    5249..5273
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
                     /label="lac operator"
     terminator      9563..9610
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
                     /note="color: #ffffff"
                     /label="T7 terminator"
     promoter        complement(4844..4921)
                     /gene="lacI"
                     /note=""
                     /note="color: #ffffff"
                     /label="lacI promoter"
     promoter        104..208
                     /gene="bla"
                     /label="AmpR promoter"
                     /note="color: #ffffff"
     CDS             complement(2258..2449)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /transl_table=1
                     /translation="VTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL*"
                     /label="rop"
                     /note="color: #993366"
     rep_origin      1240..1828
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /label="ori"
     CDS             209..1069
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ccffcc"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     CDS             complement(3761..4843)
                     /codon_start=1
                     /gene="lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /note="color: #993366"
                     /product="lac repressor"
                     /transl_table=1
                     /translation="VKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ*"
                     /label="lacI"
     CDS             5317..9420
                     /codon_start=1
                     /locus_tag=""
                     /note="generates RNA-guided double strand breaks in DNA"
                     /note="color: #993366"
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus
                     pyogenes Type II CRISPR/Cas system"
                     /protein_id=""
                     /transl_table=1
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
                     /label="Cas9"
ORIGIN
        1 ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
       61 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
      121 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
      181 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
      241 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
      301 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
      361 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
      421 agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg
      481 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
      541 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
      601 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
      661 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
      721 accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact
      781 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
      841 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
      901 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
      961 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     1021 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     1081 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     1141 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     1201 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     1261 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     1321 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     1381 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     1441 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     1501 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     1561 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     1621 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     1681 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     1741 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     1801 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     1861 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     1921 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     1981 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
     2041 tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc
     2101 gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc
     2161 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     2221 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     2281 cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga
     2341 tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc
     2401 ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg
     2461 tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca
     2521 cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac
     2581 tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg
     2641 ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga
     2701 acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga
     2761 agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc
     2821 gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg
     2881 tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga
     2941 tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg
     3001 tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc
     3061 cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg
     3121 acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt
     3181 ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt
     3241 taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg
     3301 cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat
     3361 aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc
     3421 ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt
     3481 gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat
     3541 cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg
     3601 tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg
     3661 cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gagatcccgg
     3721 tgcctaatga gtgagctaac ttacattaat tgcgttgcgc tcactgcccg ctttccagtc
     3781 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     3841 gcgtattggg cgccagggtg gtttttcttt tcaccagtga gacgggcaac agctgattgc
     3901 ccttcaccgc ctggccctga gagagttgca gcaagcggtc cacgctggtt tgccccagca
     3961 ggcgaaaatc ctgtttgatg gtggttaacg gcgggatata acatgagctg tcttcggtat
     4021 cgtcgtatcc cactaccgag atatccgcac caacgcgcag cccggactcg gtaatggcgc
     4081 gcattgcgcc cagcgccatc tgatcgttgg caaccagcat cgcagtggga acgatgccct
     4141 cattcagcat ttgcatggtt tgttgaaaac cggacatggc actccagtcg ccttcccgtt
     4201 ccgctatcgg ctgaatttga ttgcgagtga gatatttatg ccagccagcc agacgcagac
     4261 gcgccgagac agaacttaat gggcccgcta acagcgcgat ttgctggtga cccaatgcga
     4321 ccagatgctc cacgcccagt cgcgtaccgt cttcatggga gaaaataata ctgttgatgg
     4381 gtgtctggtc agagacatca agaaataacg ccggaacatt agtgcaggca gcttccacag
     4441 caatggcatc ctggtcatcc agcggatagt taatgatcag cccactgacg cgttgcgcga
     4501 gaagattgtg caccgccgct ttacaggctt cgacgccgct tcgttctacc atcgacacca
     4561 ccacgctggc acccagttga tcggcgcgag atttaatcgc cgcgacaatt tgcgacggcg
     4621 cgtgcagggc cagactggag gtggcaacgc caatcagcaa cgactgtttg cccgccagtt
     4681 gttgtgccac gcggttggga atgtaattca gctccgccat cgccgcttcc actttttccc
     4741 gcgttttcgc agaaacgtgg ctggcctggt tcaccacgcg ggaaacggtc tgataagaga
     4801 caccggcata ctctgcgaca tcgtataacg ttactggttt cacattcacc accctgaatt
     4861 gactctcttc cgggcgctat catgccatac cgcgaaaggt tttgcgccat tcgatggtgt
     4921 ccgggatctc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg
     4981 ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac
     5041 agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg
     5101 aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca
     5161 cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc
     5221 ccgcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat tcccctctag
     5281 aaataatttt gtttaacttt aagaaggaga tataccatgg ataaaaaata cagcattggt
     5341 ctggacattg gcacgaatag cgttggttgg gcagtgatta ccgatgaata caaagtcccg
     5401 tcgaaaaaat tcaaagtgct gggtaacacc gatcgccata gcattaagaa aaacctgatc
     5461 ggtgcgctgc tgtttgattc tggcgaaacc gcggaagcaa cgcgtctgaa acgtaccgca
     5521 cgtcgccgtt acacgcgccg taaaaatcgt atttgctatc tgcaggaaat ctttagcaac
     5581 gaaatggcga aagtcgatga ctcatttttc caccgcctgg aagaatcgtt tctggtggaa
     5641 gaagataaaa aacatgaacg tcacccgatt ttcggcaata tcgttgatga agtcgcgtac
     5701 catgaaaaat atccgacgat ttaccacctg cgtaaaaaac tggtggattc taccgacaaa
     5761 gccgatctgc gcctgattta tctggcactg gctcatatga tcaaatttcg tggtcacttc
     5821 ctgattgaag gcgacctgaa cccggataat agtgacgtcg ataaactgtt tattcagctg
     5881 gtgcaaacct ataatcagct gttcgaagaa aacccgatca atgcaagtgg tgttgatgcg
     5941 aaagccattc tgtccgctcg cctgagtaaa tcccgccgtc tggaaaacct gattgcacag
     6001 ctgccgggtg aaaagaaaaa cggtctgttt ggcaatctga tcgctctgtc actgggcctg
     6061 acgccgaact ttaaatcgaa tttcgacctg gcagaagatg ctaaactgca gctgagcaaa
     6121 gatacctacg atgacgatct ggacaacctg ctggcgcaaa ttggcgacca gtatgccgac
     6181 ctgtttctgg cggccaaaaa tctgtcagat gccattctgc tgtcggacat cctgcgcgtg
     6241 aacaccgaaa tcacgaaagc gccgctgtca gcctcgatga ttaaacgcta cgatgaacat
     6301 caccaggacc tgaccctgct gaaagcactg gttcgtcagc aactgccgga aaaatacaaa
     6361 gaaattttct ttgaccaaag taaaaatggt tatgcaggct acatcgatgg cggtgcttcc
     6421 caggaagaat tctacaaatt catcaaaccg atcctggaaa aaatggatgg tacggaagaa
     6481 ctgctggtga aactgaatcg tgaagatctg ctgcgtaaac aacgcacctt tgacaacggt
     6541 agcattccgc atcagatcca cctgggcgaa ctgcatgcga ttctgcgccg tcaggaagat
     6601 ttttatccgt tcctgaaaga caaccgtgaa aaaatcgaaa aaatcctgac gtttcgcatc
     6661 ccgtattacg ttggtccgct ggcacgtggt aatagccgct tcgcatggat gacccgcaaa
     6721 tctgaagaaa ccattacgcc gtggaacttt gaagaagtgg ttgataaagg cgcaagcgct
     6781 cagtctttta tcgaacgtat gaccaatttc gataaaaacc tgccgaatga aaaagtgctg
     6841 ccgaaacatt ctctgctgta tgaatacttt accgtttaca acgaactgac gaaagtgaaa
     6901 tatgttaccg agggtatgcg caaaccggcg tttctgagtg gcgaacagaa aaaagccatt
     6961 gtggatctgc tgttcaaaac caatcgtaaa gttacggtca aacagctgaa agaagattac
     7021 ttcaagaaaa ttgaatgttt cgacagcgtg gaaatttctg gtgttgaaga tcgtttcaac
     7081 gcctctctgg gcacctatca tgacctgctg aaaatcatca aagacaaaga ttttctggat
     7141 aacgaagaaa acgaagacat tctggaagat atcgtgctga ccctgacgct gttcgaagat
     7201 cgtgaaatga ttgaagaacg cctgaaaacg tacgcacacc tgtttgacga taaagttatg
     7261 aaacagctga aacgccgtcg ctataccggt tggggccgtc tgagccgcaa actgattaat
     7321 ggtatccgcg ataaacaatc aggcaaaacg attctggatt tcctgaaatc ggacggcttt
     7381 gccaaccgta atttcatgca gctgatccat gacgattccc tgacctttaa agaagacatt
     7441 cagaaagcac aagtgtcagg tcaaggcgat tcgctgcatg aacacattgc gaacctggcc
     7501 ggttcaccgg ctatcaaaaa aggcatcctg cagaccgtga aagtcgtgga tgaactggtg
     7561 aaagttatgg gtcgtcacaa accggaaaac attgttatcg aaatggcgcg cgaaaatcag
     7621 accacgcaaa aaggccagaa aaactcgcgt gaacgcatga aacgcattga agaaggtatc
     7681 aaagaactgg gcagccagat tctgaaagaa catccggtcg aaaacaccca gctgcaaaat
     7741 gaaaaactgt acctgtatta cctgcaaaat ggtcgtgaca tgtatgtgga tcaggaactg
     7801 gacatcaacc gcctgtctga ctatgatgtc gaccacattg tgccgcagag ctttctgaaa
     7861 gacgattcta tcgataacaa agttctgacc cgtagtgata aaaaccgcgg caaaagcgac
     7921 aatgtcccgt ctgaagaagt tgtgaagaaa atgaaaaact actggcgtca actgctgaat
     7981 gcgaaactga ttacgcagcg taaattcgat aacctgacca aagcggaacg cggcggtctg
     8041 tccgaactgg ataaagccgg ttttatcaaa cgtcaactgg ttgaaacccg ccagattacg
     8101 aaacatgtcg cccagatcct ggattcacgc atgaacacga aatacgacga aaacgataaa
     8161 ctgatccgtg aagtcaaagt gatcaccctg aaaagtaaac tggtttccga tttccgtaaa
     8221 gactttcagt tctacaaagt ccgcgaaatt aacaattacc atcacgcaca cgatgcttat
     8281 ctgaatgcag tggttggtac cgctctgatc aaaaaatatc cgaaactgga aagcgaattt
     8341 gtgtatggcg attacaaagt ctatgacgtg cgcaaaatga ttgcgaaatc cgaacaggaa
     8401 atcggcaaag cgaccgccaa atactttttc tattcaaaca tcatgaactt tttcaaaacc
     8461 gaaattacgc tggcaaatgg tgaaattcgt aaacgcccgc tgatcgaaac caacggtgaa
     8521 acgggcgaaa ttgtgtggga taaaggccgt gacttcgcga ccgttcgcaa agtcctgtcg
     8581 atgccgcaag tgaatatcgt gaagaaaacc gaagtgcaga cgggcggttt tagtaaagaa
     8641 tccatcctgc cgaaacgtaa cagcgataaa ctgattgcgc gcaaaaaaga ttgggacccg
     8701 aaaaaatacg gcggttttga tagtccgacg gttgcatatt ccgtcctggt cgtggctaaa
     8761 gtcgaaaaag gtaaaagtaa aaaactgaaa tccgtgaaag aactgctggg cattaccatc
     8821 atggaacgta gctcttttga gaaaaacccg attgacttcc tggaagccaa aggttacaaa
     8881 gaagtgaaaa aagatctgat catcaaactg ccgaaatata gcctgttcga actggaaaac
     8941 ggccgtaaac gcatgctggc atctgctggt gaactgcaga aaggcaatga actggcactg
     9001 ccgagtaaat atgttaactt tctgtacctg gctagccatt atgaaaaact gaaaggttct
     9061 ccggaagata acgaacagaa acaactgttc gtcgaacaac ataaacacta cctggatgaa
     9121 atcatcgaac agatctcaga attctcgaaa cgcgtgattc tggcggatgc caatctggac
     9181 aaagttctga gcgcgtataa caaacatcgt gataaaccga ttcgcgaaca ggccgaaaat
     9241 attatccacc tgtttaccct gacgaacctg ggcgcaccgg cagcttttaa atacttcgat
     9301 accacgatcg accgtaaacg ctatacctca acgaaagaag ttctggatgc taccctgatt
     9361 catcaatcga tcaccggtct gtatgaaacg cgtattgatc tgagtcagct gggcggtgac
     9421 ggcggtggct ccggcacccg tctgccgaaa aagaaacgca aagtgggcgg tggctcgcat
     9481 catcatcatc atcactaagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc
     9541 tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag
     9601 gggttttttg ctgaaaggag gaactatatc cggatatccc gcaagaggcc cggcagtacc
     9661 ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc
     9721 gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta
     9781 ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
//
