LOCUS       .                       6442 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             complement(3382..4242)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ccffcc"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     CDS             530..1129
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /note="color: #ccffcc"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     rep_origin      complement(2623..3211)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /label="ori"
     promoter        10..509
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /note="color: #ffffff"
                     /label="PGK promoter"
     LTR             1310..1735
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
                     /note="color: #ffe4c4"
                     /label="3' LTR (ΔU3)"
     CDS             5755..6171
                     /codon_start=1
                     /note="truncated MMLV gag gene lacking the start codon"
                     /note="color: #993366"
                     /transl_table=1
                     /label="gag (truncated)"
     enhancer        4533..4912
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
                     /label="CMV enhancer"
     promoter        2001..2330
                     /note="SV40 enhancer and early promoter"
                     /note="color: #ffffff"
                     /label="SV40 promoter"
     promoter        4913..5116
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /note="color: #ffffff"
                     /label="CMV promoter"
     misc_feature    5355..5554
                     /note="packaging signal of Moloney murine leukemia virus
                     (MMLV)"
                     /note="color: #ffe4c4"
                     /label="MMLV Ψ"
     LTR             5117..5292
                     /note="truncated long terminal repeat from Moloney murine
                     sarcoma virus"
                     /note="color: #ffe4c4"
                     /label="5' LTR (truncated)"
     rep_origin      2181..2316
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
                     /label="SV40 ori"
     promoter        complement(4243..4347)
                     /gene="bla"
                     /label="AmpR promoter"
                     /note="color: #ffffff"
     promoter        6195..6435
                     /note="Truncated RNA polymerase III promoter for human U6
                     snRNA used in pSIREN vectors such as
                     pSIREN-RetroQ-ZsGreen1. Expected transcription start site
                     is 8bp downstream of this feature."
                     /note="color: #ffffff"
                     /label="U6 promoter"
ORIGIN
        1 aattctaccg ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag
       61 ccccgctggg cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca
      121 ccggtaggcg ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc
      181 ccctagtcag gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg
      241 aagtagcacg tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg
      301 taggcctttg gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc
      361 tgggaagggg tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc
      421 gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt
      481 tctcctcttc ctcatctccg ggcctttcga cctgcagccc aagcttacca tgaccgagta
      541 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc
      601 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga
      661 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
      721 gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc
      781 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc
      841 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
      901 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
      961 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc
     1021 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt
     1081 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac gcccgcccca
     1141 cgacccgcag cgcccgaccg aaaggagcgc acgaccccat gcatcggcgt ctcgagatat
     1201 cagtggtcca ggctctagtt ttgactcaac aatatcacca gctgaagcct atagagtacg
     1261 agccatagat aaaataaaag attttattta gtctccagaa aaagggggga atgaaagacc
     1321 ccacctgtag gtttggcaag ctagcttaag taacgccatt ttgcaaggca tggaaaaata
     1381 cataactgag aatagagaag ttcagatcaa ggtcaggaac agatggaaca gggtcgaccc
     1441 tagagaacca tcagatgttt ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat
     1501 ttgaactaac caatcagttc gcttctcgct tctgttcgcg cgcttctgct ccccgagctc
     1561 aataaaagag cccacaaccc ctcactcggg gcgccagtcc tccgattgac tgagtcgccc
     1621 gggtacccgt gtatccaata aaccctcttg cagttgcatc cgacttgtgg tctcgctgtt
     1681 ccttgggagg gtctcctctg agtgattgac tacccgtcag cgggggtctt tcatttgggg
     1741 gctcgtccgg gatcgggaga cccctgccca gggaccaccg acccaccacc gggaggtaag
     1801 ctggctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
     1861 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
     1921 cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag
     1981 tgtagatccg gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     2041 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     2101 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     2161 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     2221 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     2281 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagcttactg
     2341 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat
     2401 accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt cctcgctcac
     2461 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     2521 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     2581 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     2641 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     2701 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     2761 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     2821 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     2881 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     2941 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     3001 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     3061 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     3121 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     3181 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     3241 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     3301 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     3361 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     3421 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     3481 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     3541 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     3601 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     3661 gccagttaat agtttgcgca acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc
     3721 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     3781 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     3841 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     3901 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     3961 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca cgggataata ccgcgccaca
     4021 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     4081 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     4141 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     4201 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     4261 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     4321 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
     4381 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
     4441 tcttcaagaa ttagcttggc cattgcatac gttgtatcca tatcataata tgtacattta
     4501 tattggctca tgtccaacat taccgccatg ttgacattga ttattgacta gttattaata
     4561 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
     4621 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
     4681 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
     4741 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
     4801 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
     4861 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
     4921 gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
     4981 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
     5041 atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt
     5101 ctatataagc agagctcaat aaaagagccc acaacccctc actcggcgcg ccagtcttcc
     5161 gatagactgc gtcgcccggg tacccgtatt cccaataaag cctcttgctg tttgcatccg
     5221 aatcgtggtc tcgctgttcc ttgggagggt ctcctctgag tgattgacta cccacgacgg
     5281 gggtctttca tttgggggct cgtccgggat ttggagaccc ctgcccaggg accaccgacc
     5341 caccaccggg aggtaagctg gccagcaact tatctgtgtc tgtccgattg tctagtgtct
     5401 atgtttgatg ttatgcgcct gcgtctgtac tagttagcta actagctctg tatctggcgg
     5461 acccgtggtg gaactgacga gttctgaaca cccggccgca accctgggag acgtcccagg
     5521 gactttgggg gccgtttttg tggcccgacc tgaggaaggg agtcgatgtg gaatccgacc
     5581 ccgtcaggat atgtggttct ggtaggagac gagaacctaa aacagttccc gcctccgtct
     5641 gaatttttgc tttcggtttg gaaccgaagc cgcgcgtctt gtctgctgca gcgctgcagc
     5701 atcgttctgt gttgtctctg tctgactgtg tttctgtatt tgtctgaaaa ttagggccag
     5761 actgttacca ctcccttaag tttgacctta ggtcactgga aagatgtcga gcggatcgct
     5821 cacaaccagt cggtagatgt caagaagaga cgttgggtta ccttctgctc tgcagaatgg
     5881 ccaaccttta acgtcggatg gccgcgagac ggcaccttta accgagacct catcacccag
     5941 gttaagatca aggtcttttc acctggcccg catggacacc cagaccaggt cccctacatc
     6001 gtgacctggg aagccttggc ttttgacccc cctccctggg tcaagccctt tgtacaccct
     6061 aagcctccgc ctcctcttcc tccatccgcc ccgtctctcc cccttgaacc tcctcgttcg
     6121 accccgcctc gatcctccct ttatccagcc ctcactcctt ctctaggcgc cggaattgaa
     6181 gatctgggca ggaagagggc ctatttccca tgattccttc atatttgcat atacgataca
     6241 aggctgttag agagataatt agaattaatt tgactgtaaa cacaaagata ttagtacaaa
     6301 atacgtgacg tagaaagtaa taatttcttg ggtagtttgc agttttaaaa ttatgtttta
     6361 aaatggacta tcatatgctt accgtaactt gaaagtattt cgatttcttg gctttatata
     6421 tcttgtggaa aggacgagga tc
//
