LOCUS       .                       9263 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             86..1276
                     /codon_start=1
                     /gene="tet"
                     /note="confers resistance to tetracycline"
                     /note="color: #ccffcc"
                     /product="tetracycline efflux protein"
                     /transl_table=1
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST*"
                     /label="TcR"
     promoter        10..38
                     /note="promoter for tetracycline efflux protein gene"
                     /note="color: #ffffff"
                     /label="tet promoter"
     CDS             5273..6223
                     /codon_start=1
                     /gene="rep101"
                     /product="protein needed for replication with the pSC101
                     origin"
                     /transl_table=1
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI*"
                     /label="Rep101"
                     /note="color: #993366"
     rep_origin      5003..5225
                     /note="low-copy replication origin that requires the Rep101
                     protein"
                     /note="color: #ffff00"
                     /label="pSC101 ori"
ORIGIN
        1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
       61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
      121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
      181 gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
      241 tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
      301 ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
      361 cacacccgtc ctgtggatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac
      421 aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca
      481 cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg
      541 actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct
      601 caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaccgat
      661 gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt
      721 cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct
      781 ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct
      841 tgcggtattc ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa
      901 acgtttcggc gagaagcagg ccattatcgc cggcatggcg gccgacgcgc tgggctacgt
      961 cttgctggcg ttcgcgacgc gaggctggat ggccttcccc attatgattc ttctcgcttc
     1021 cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag atgacgacca
     1081 tcagggacag cttcaaggat cgctcgcggc tcttaccagc ctaacttcga tcactggacc
     1141 gctgatcgtc acggcgattt atgccgcctc ggcgagcaca tggaacgggt tggcatggat
     1201 tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg
     1261 ggccacctcg acctgaatgg aagccggcgg cacctcgcta acggattcac cactccaaga
     1321 attggagcca atcaattctt gcggagaact gtgaatgcgc aaaccaaccc ttggcagaac
     1381 atatccatcg cgtccgccat ctccagcagc cgcacgcggc gcatctcggg cagcgttggg
     1441 tcctggccac gggtgcgcat gatcgtgctc ctgtcgttga ggacccggct aggctggcgg
     1501 ggttgcctta ctggttagca gaatgaatca ccgatacgcg agcgaacgtg aagcgactgc
     1561 tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt ccgtgtttcg
     1621 taaagtctgg aaacgcggaa gtcccctacg tgctgctgaa gttgcccgca acagagagtg
     1681 gaaccaaccg gtgataccac gatactatga ctgagagtca acgccatgag cggcctcatt
     1741 tcttattctg agttacaaca gtccgcaccg ctgccggtag ctacttgact atccggctgc
     1801 actagccctg cgtcagatgg ctctgatcca aggcaaactg ccaaaatatc tgctggcacc
     1861 ggaagtcagc gccctgcacc attatgttcc ggatctgcat cgcaggatgc tgctggctac
     1921 cctgtggaac acctacatct gtattaacga agcgctggca ttgaccctga gtgatttttc
     1981 tctggtgccg ccctatccct ttgtgcagct tgccacgctc aaaggggttt gaggtccaac
     2041 cgtacgaaaa cgtacggtaa gaggaaaatt atcgtctgaa aaatcgatta gtagacaaga
     2101 aagtccgtta agtgccaatt ttcgattaaa aagacaccgt tttgatggcg ttttccaatg
     2161 tacattatgt ttcgatatat cagacagtta cttcactaac gtacgttttc gttctattgg
     2221 ccttcagacc ccatatcctt aatgtccttt atttgctggg gttatcagat ccccccgaca
     2281 cgtttaatta atgctttctc cgccggagat cgacgcacag gcttctgtgt ctatgatgtt
     2341 atttcttaat aatcatccag gtattctctt tatcaccata cgtagtgcga gtgtccacct
     2401 taacgcaggg ctttccgtca cagcgcgata tgtcagccag cggggctttc ttttgccaga
     2461 ccgcttccat cctctgcatt tcagcaatct ggctataccc gtcattcata aaccacgtaa
     2521 atgccgtcac gcaggaagcc aggacgaaga atatcgtcag tacaagataa atcgcggatt
     2581 tccacgtata gcgtgacatc tcacgacgca tttcatggat catcgctttc gccgtatcgg
     2641 cagcctgatt cagcgcttct gtcgccggtt tctgctgtgc taatccggct tgtttcagtt
     2701 ctttctcaac ctgagtgagc gcggaactca ccgatttcct gacggtgtca gtcatattac
     2761 cggacgcgct gtccagctca cgaatgaccc tgctcagcgt ttcactttgc tgctgtaatt
     2821 gtgatgaggc ggcctgaaac tgttctgtca gagaagtaac acgcttttcc agcgcctgat
     2881 gatgcccgat aagggcggca atttgtttaa tttcgtcgct catacaaaat cctgcctatc
     2941 gtgagaatga ccagccttta tccggcttct gtcgtatctg ttcggcgagt cgctgtcgtt
     3001 ctttctcctg ctgacgctgt ttttccgcca gacgttcgcg ctctctctgc ctttccatct
     3061 cctgatgtat cccctggaac tccgccatcg catcgttaac aagggactga agatcgattt
     3121 cttcctgtat atccttcatg gcatcactga ccagtgcgtt cagcttgtca ggctcttttt
     3181 caaaatcaaa cgttctgccg gaatgggatt cctgctcagg ctctgacttc agctcctgtt
     3241 ttagcgtcag agtatccctc tcgctgaggg cttcccgtaa cgaggtagtc acgtcaatta
     3301 cgctgtcacg ttcatcacgg gactgctgca cctgcctttc agcctccctg cgctcaagaa
     3361 tggcctgtag ctgctcagta tcgaatcgct gaacctgacc cgcgcccaga tgccgctcag
     3421 gctcacggtc aatgccctgc gccttcaggg aacgggaatc aacccggtca gcgtgctgat
     3481 accgttcaag gtgcttattc tggaggtcag cccagcgtct ccctctgggc aacaaggtat
     3541 tctttgcgtt cggtcggtgt ttccccgaaa cgtgcctttt ttgcgccacc gcgtccggct
     3601 ctttggtgtt agcccgttta aaatactgct cagggtcacg gtgaataccg tcattaatgc
     3661 gttcagagaa catgatatgg gcgtggggct gctcgccacc ggctatcgct gctttcggat
     3721 tatggatagc gaactgatag gcatggcggt cgccaatttc ctgttggaca aaatcgcgga
     3781 caagctcaag acgttgttcg ggttttaact cacgcggcag ggcaatctcg atttcacggt
     3841 aggtacagcc gttggcacgt tcagacgtgt cagcggcttt ccagaactcg gacggtttat
     3901 gcgctgccca cgccggcata ttgccggact ccttgtgctc aaggtcggag tctttttcac
     3961 gggcatactt tccctcacgc gcaatataat cggcatgagg agaggcactg ccttttccgc
     4021 cggtttttac gctgagatga taggatgcca tcgtgtttta tcccgctgaa gggcgcacgt
     4081 ttctgaacga agtgaagaaa gtctaagtgc gccctgataa ataaaagagt tatcagggat
     4141 tgtagtggga tttgacctcc tctgccatca tgagcgtaat cattccgtta gcattcagga
     4201 ggtaaacagc atgaataaaa gcgaaaaaac aggaacaatg ggcagcagaa agagtgcagt
     4261 atattcgcgg cttaaagtcg ccgaatgagc aacagaaact tatgctgata ctgacggata
     4321 aagcagataa aacagcacag gatatcaaaa cgctgtccct gctgatgaag gctgaacagg
     4381 cagcagagaa agcgcaggaa gccagagcga aagtcatgaa cctgatacag gcagaaaagc
     4441 gagccgaagc cagagccgcc cgtaaagccc gtgaccatgc tctgtaccag tctgccggat
     4501 tgcttatcct ggcgggtctg gttgacagta agacgggtaa gcctgttgat gataccgctg
     4561 ccttactggg tgcattagcc agtctgaatg acctgtcacg ggataatccg aagtggtcag
     4621 actggaaaat cagagggcag gaactgctga acagcaaaaa gtcagatagc accacatagc
     4681 agacccgcca taaaacgccc tgagaagccc gtgacgggct tttcttgtat tatgggtagt
     4741 ttccttgcat gaatccataa aaggcgcctg tagtgccatt tacccccatt cactgccaga
     4801 gccgtgagcg cagcgaactg aatgtcacga aaaagacagc gactcaggtg cctgatggtc
     4861 ggagacaaaa ggaatattca gcgatttgcc cgagcttgcg agggtgctac ttaagccttt
     4921 agggttttaa ggtctgtttt gtagaggagc aaacagcgtt tgcgacatcc ttttgtaata
     4981 ctgcggaact gactaaagta gtgagttata cacagggctg ggatctattc tttttatctt
     5041 tttttattct ttctttattc tataaattat aaccacttga atataaacaa aaaaaacaca
     5101 caaaggtcta gcggaattta cagagggtct agcagaattt acaagttttc cagcaaaggt
     5161 ctagcagaat ttacagatac ccacaactca aaggaaaagg actagtaatt atcattgact
     5221 agcccatctc aattggtata gtgattaaaa tcacctagac caattgagat gtatgtctga
     5281 attagttgtt ttcaaagcaa atgaactagc gattagtcgc tatgacttaa cggagcatga
     5341 aaccaagcta attttatgct gtgtggcact actcaacccc acgattgaaa accctacaag
     5401 gaaagaacgg acggtatcgt tcacttataa ccaatacgct cagatgatga acatcagtag
     5461 ggaaaatgct tatggtgtat tagctaaagc aaccagagag ctgatgacga gaactgtgga
     5521 aatcaggaat cctttggtta aaggctttga gattttccag tggacaaact atgccaagtt
     5581 ctcaagcgaa aaattagaat tagtttttag tgaagagata ttgccttatc ttttccagtt
     5641 aaaaaaattc ataaaatata atctggaaca tgttaagtct tttgaaaaca aatactctat
     5701 gaggatttat gagtggttat taaaagaact aacacaaaag aaaactcaca aggcaaatat
     5761 agagattagc cttgatgaat ttaagttcat gttaatgctt gaaaataact accatgagtt
     5821 taaaaggctt aaccaatggg ttttgaaacc aataagtaaa gatttaaaca cttacagcaa
     5881 tatgaaattg gtggttgata agcgaggccg cccgactgat acgttgattt tccaagttga
     5941 actagataga caaatggatc tcgtaaccga acttgagaac aaccagataa aaatgaatgg
     6001 tgacaaaata ccaacaacca ttacatcaga ttcctaccta cgtaacggac taagaaaaac
     6061 actacacgat gctttaactg caaaaattca gctcaccagt tttgaggcaa aatttttgag
     6121 tgacatgcaa agtaagcatg atctcaatgg ttcgttctca tggctcacgc aaaaacaacg
     6181 aaccacacta gagaacatac tggctaaata cggaaggatc tgaggttctt atggctcttg
     6241 tatctatcag tgaagcatca agactaacaa acaaaagtag aacaactgtt caccgttaga
     6301 tatcaaaggg aaaactgtcc atatgcacag atgaaaacgg tgtaaaaaag atagatacat
     6361 cagagctttt acgagttttt ggtgcattta aagctgttca ccatgaacag atcgacaatg
     6421 taacagatga acagcatgta acacctaata gaacaggtga aaccagtaaa acaaagcaac
     6481 tagaacatga aattgaacac ctgagacaac ttgttacagc tcaacagtca cacatagaca
     6541 gcctgaaaca ggcgatgctg cttatcgaat caaagctgcc gacaacacgg gagccagtga
     6601 cgcctcccgt ggggaaaaaa tcatggcaat tctggaagaa atagcgcttt cagccggcaa
     6661 acctgaagcc ggatctgcga ttctgataac aaactagcaa caccagaaca gcccgtttgc
     6721 gggcagcaaa acccgtactt ttggacgttc cggcggtttt ttgtggcgag tggtgttcgg
     6781 gcggtgcgcg caagatccat tatgttaaac gggcgagttt acatctcaaa accgcccgct
     6841 taacaccatc agaaatcctc agcgcgattt taagcaccaa cccccccccg taacacccaa
     6901 atccatactg aaagtggctt tgttgaataa atcgaacttt tgctgagttg aaggatcaga
     6961 tcacgcatcc tcccgacaac acagaccatt ccgtggcaaa gcaaaagttc agaatcacca
     7021 actggtccac ctacaacaaa gctctcatca accgtggctc cctcactttc tggctggatg
     7081 atgaggcgat tcaggcctgg tatgagtcgg caacaccttc atcacgagga aggccccagc
     7141 gctattctga tctcgccatc accaccgttc tggtgattaa acgcgtattc cggctgaccc
     7201 tgcgggctgc gcagggtttt attgattcca tttttgccct gatgaacgtt ccgttgcgct
     7261 gcccggatta caccagtgtc agtaagcggg caaagtcggt taatgtcagt ttcaaaacgt
     7321 ccacccgggg tgaaatcgca cacctggtga ttgattccac cgggctgaag gtctttggtg
     7381 aaggcgaatg gaaagtcaga aagcacggca aagagcgccg tcgtatctgg cgaaagttgc
     7441 atcttgctgt tgacagcaac acacatgaag ttgtctgtgc agacctgtcg ctgaataacg
     7501 tcacggactc agaagccttc ccgggcctta tccggcagac tcacagaaaa atcagggcag
     7561 ccgcggcaga cggggcttac gatacccggc tctgtcacga tgaactgcgc cgcaaaaaaa
     7621 tcagcgcgct tattcctccc cgaaaaggtg cgggttactg gcccggtgaa tatgcagacc
     7681 gtaaccgtgc agtggctaat cagcgaatga ccgggagtaa tgcgcggtgg aaatggacaa
     7741 cagattacaa ccgtcgctcg atagcggaaa cggcgatgta ccgggtaaaa cagctgttcg
     7801 ggggttcact gacgctgcgt gactacgatg gtcaggttgc ggaggctatg gccctggtac
     7861 gagcgctgaa caaaatgacg aaagcaggta tgcctgaaag cgtgcgtatt gcctgaaaac
     7921 acaacccgct acgggggaga cttacccgaa atctgattta ttcaacaaag ccgggtgtgg
     7981 tgaactacaa agcagacccg ttgaggttat cagttcgatg cacaatcagc agcgcataaa
     8041 atatgcacaa gaacaggagc acccttcgca ttaagctgtg gtggtaacaa gtagtgccgg
     8101 gctaccatca gcgagcatga tgcgctccca cagcattcgc cttggcagta tggaagttcc
     8161 tcgctccagt tcgggccggt atccacctcg agtgacccca gccgcccctc atgccaaccg
     8221 agcccattaa gcgcagagtc ggccgcaact atctcgggga cgcataaaca cgcagcgatt
     8281 taccaaggag ttcggctagg tcaatccgca attccctttg cctggccgga gatgccgcga
     8341 gcaaacaatg tgtccggtcg ctaccagatg gactttgtgg gcggaggatt gcaccagacg
     8401 cttcagcgca gcgtcgtacg atgaatcgga aggcccggta agcaattgaa gaaccgctga
     8461 ccacctccca tccgacaatg caagggtgcc ccgctcccag cgcgacacgg ttgcctgatc
     8521 cacacccatt aattcagcta ggtgactttg cttcatatga cgaagcaacc gcgccctccg
     8581 cacctgccgc cccctgtcat tcgtcaacat tcttcagcac ctcaatgtcg ttcgtagtga
     8641 gcctcatttt ttcaagaccg ccgatgatga gagccaggcc tcgctcgaat gccgcgtccg
     8701 gaccgccttc gtagacgatt ttcatcgcgc tctgtaggcg cgccggcatc gtagacgctg
     8761 aggtggtcaa ctgatcttcg ccccgctcct cggcgtctgc ctcgctagct tgctgctcaa
     8821 gaacagcgcc gacggtgaag tagctgattg ccatcaacgc ataggtcgcg tcacctgccg
     8881 aaaagccagc atcgcaaagg aagcgaagct gcgcgtcggc tttttccatc tgcggcgcgg
     8941 ctggccgcgt cccggcatga atacgcgcgc catcgcgata agcgagcaac gcccgtcgaa
     9001 aactgcatgc attgcccttc aggaacgaac gccagtcgtc gtcatccctt ggcgtcgaat
     9061 gcgtgtgatt tatcgtcagc atggcttcgg caagtgcgtc gagcaacgca cgcttgttct
     9121 tgaaatgcca gtagagcgct ggctgttgca ccccgaggcg ctcagccagt cggcgcgtcg
     9181 ttagaccttc catgcccacg tcgttaagca gttcgagcgc ggttcggatc acggcctcgc
     9241 gttggagctt gttcattcgc gaa
//
