LOCUS       .                       3597 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             1324..1341
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /transl_table=1
                     /translation="HHHHHH"
                     /label="6xHis"
                     /note="color: #cc99b2"
     CDS             1279..1308
                     /codon_start=1
                     /product="Myc (human c-Myc oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
                     /note="color: #cc99b2"
     promoter        2101..2148
                     /note="synthetic bacterial promoter"
                     /note="color: #ffffff"
                     /label="EM7 promoter"
     terminator      1421..1667
                     /gene="Pichia pastoris AOX1"
                     /note="transcription terminator for AOX1"
                     /note="color: #ffffff"
                     /label="AOX1 terminator"
     terminator      2607..2854
                     /gene="S. cerevisiae CYC1"
                     /note="transcription terminator for CYC1"
                     /note="color: #ffffff"
                     /label="CYC1 terminator"
     CDS             941..1207
                     /codon_start=1
                     /gene="MFα1"
                     /note="Cleavage by the Kex2 protease occurs after the
                     dibasic KR sequence. The EA dipeptides are then removed by
                     dipeptidyl aminopeptidase A."
                     /note="color: #993366"
                     /product="N-terminal secretion signal from S. cerevisiae
                     alpha-factor"
                     /transl_table=1
                     /translation="MRFPSIFTAVLFAASSALA,APVNTTTEDETAQIPAEAVIGYSDL
                     EGDFDVAVLPFSNSTNNGLLFINTTIASIAAKEEGVSLEKR,EAEA"
                     /label="α-factor secretion signal"
     CDS             2167..2541
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /note="confers resistance to bleomycin, phleomycin, and
                     Zeocin™"
                     /note="color: #ccffcc"
                     /product="antibiotic-binding protein"
                     /transl_table=1
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD*"
                     /label="BleoR"
     rep_origin      complement(2929..3517)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /label="ori"
     promoter        2..940
                     /gene="Pichia pastoris AOX1"
                     /note="inducible promoter, regulated by methanol"
                     /note="color: #ffffff"
                     /label="AOX1 promoter"
     promoter        1707..2093
                     /gene="S. cerevisiae TEF1"
                     /note="promoter for EF-1α"
                     /note="color: #ffffff"
                     /label="TEF1 promoter"
     misc_feature    1206..1280
                     /note="multiple cloning site"
                     /note="color: #99ccff"
                     /label="MCS"
ORIGIN
        1 agatctaaca tccaaagacg aaaggttgaa tgaaaccttt ttgccatccg acatccacag
       61 gtccattctc acacataagt gccaaacgca acaggagggg atacactagc agcagaccgt
      121 tgcaaacgca ggacctccac tcctcttctc ctcaacaccc acttttgcca tcgaaaaacc
      181 agcccagtta ttgggcttga ttggagctcg ctcattccaa ttccttctat taggctacta
      241 acaccatgac tttattagcc tgtctatcct ggcccccctg gcgaggttca tgtttgttta
      301 tttccgaatg caacaagctc cgcattacac ccgaacatca ctccagatga gggctttctg
      361 agtgtggggt caaatagttt catgttcccc aaatggccca aaactgacag tttaaacgct
      421 gtcttggaac ctaatatgac aaaagcgtga tctcatccaa gatgaactaa gtttggttcg
      481 ttgaaatgct aacggccagt tggtcaaaaa gaaacttcca aaagtcggca taccgtttgt
      541 cttgtttggt attgattgac gaatgctcaa aaataatctc attaatgctt agcgcagtct
      601 ctctatcgct tctgaacccc ggtgcacctg tgccgaaacg caaatgggga aacacccgct
      661 ttttggatga ttatgcattg tctccacatt gtatgcttcc aagattctgg tgggaatact
      721 gctgatagcc taacgttcat gatcaaaatt taactgttct aacccctact tgacagcaat
      781 atataaacag aaggaagctg ccctgtctta aacctttttt tttatcatca ttattagctt
      841 actttcataa ttgcgactgg ttccaattga caagcttttg attttaacga cttttaacga
      901 caacttgaga agatcaaaaa acaactaatt attcgaaacg atgagatttc cttcaatttt
      961 tactgctgtt ttattcgcag catcctccgc attagctgct ccagtcaaca ctacaacaga
     1021 agatgaaacg gcacaaattc cggctgaagc tgtcatcggt tactcagatt tagaagggga
     1081 tttcgatgtt gctgttttgc cattttccaa cagcacaaat aacgggttat tgtttataaa
     1141 tactactatt gccagcattg ctgctaaaga agaaggggta tctctcgaga aaagagaggc
     1201 tgaagctgca ggaattcacg tggcccagcc ggccgtctcg gatcggtacc tcgagccgcg
     1261 gcggccgcca gctttctaga acaaaaactc atctcagaag aggatctgaa tagcgccgtc
     1321 gaccatcatc atcatcatca ttgagtttgt agccttagac atgactgttc ctcagttcaa
     1381 gttgggcact tacgagaaga ccggtcttgc tagattctaa tcaagaggat gtcagaatgc
     1441 catttgcctg agagatgcag gcttcatttt tgatactttt ttatttgtaa cctatatagt
     1501 ataggatttt ttttgtcatt ttgtttcttc tcgtacgagc ttgctcctga tcagcctatc
     1561 tcgcagctga tgaatatctt gtggtagggg tttgggaaaa tcattcgagt ttgatgtttt
     1621 tcttggtatt tcccactcct cttcagagta cagaagatta agtgagacct tcgtttgtgc
     1681 ggatccccca cacaccatag cttcaaaatg tttctactcc ttttttactc ttccagattt
     1741 tctcggactc cgcgcatcgc cgtaccactt caaaacaccc aagcacagca tactaaattt
     1801 tccctctttc ttcctctagg gtgtcgttaa ttacccgtac taaaggtttg gaaaagaaaa
     1861 aagagaccgc ctcgtttctt tttcttcgtc gaaaaaggca ataaaaattt ttatcacgtt
     1921 tctttttctt gaaatttttt tttttagttt ttttctcttt cagtgacctc cattgatatt
     1981 taagttaata aacggtcttc aatttctcaa gtttcagttt catttttctt gttctattac
     2041 aacttttttt acttcttgtt cattagaaag aaagcatagc aatctaatct aaggggcggt
     2101 gttgacaatt aatcatcggc atagtatatc ggcatagtat aatacgacaa ggtgaggaac
     2161 taaaccatgg ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga
     2221 gcggtcgagt tctggaccga ccggctcggg ttctcccggg acttcgtgga ggacgacttc
     2281 gccggtgtgg tccgggacga cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg
     2341 ccggacaaca ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg
     2401 tcggaggtcg tgtccacgaa cttccgggac gcctccgggc cggccatgac cgagatcggc
     2461 gagcagccgt gggggcggga gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc
     2521 gtggccgagg agcaggactg acacgtccga cggcggccca cgggtcccag gcctcggaga
     2581 tccgtccccc ttttcctttg tcgatatcat gtaattagtt atgtcacgct tacattcacg
     2641 ccctcccccc acatccgctc taaccgaaaa ggaaggagtt agacaacctg aagtctaggt
     2701 ccctatttat ttttttatag ttatgttagt attaagaacg ttatttatat ttcaaatttt
     2761 tctttttttt ctgtacagac gcgtgtacgc atgtaacatt atactgaaaa ccttgcttga
     2821 gaaggttttg ggacgctcga aggctttaat ttgcaagctg gagaccaaca tgtgagcaaa
     2881 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     2941 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     3001 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     3061 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     3121 tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     3181 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     3241 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     3301 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     3361 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     3421 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     3481 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     3541 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagatc
//
