LOCUS       .                       4411 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        77..108
                     /note="minimal TATA-box promoter with low basal activity"
                     /note="color: #ffffff"
                     /label="minP"
     polyA_signal    4258..4306
                     /note="synthetic polyadenylation signal"
                     /note="color: #a6acb3"
                     /label="poly(A) signal"
     misc_feature    1..70
                     /note="multiple cloning site"
                     /note="color: #99ccff"
                     /label="MCS"
     misc_feature    4320..4411
                     /note="RNA polymerase II transcriptional pause signal from
                     the human α2 globin gene"
                     /note="color: #a6acb3"
                     /label="pause site"
     CDS             1794..1913
                     /codon_start=1
                     /note="human codon-optimized"
                     /note="color: #cc99b2"
                     /product="PEST degradation sequence from mouse ornithine
                     decarboxylase"
                     /transl_table=1
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
                     /label="hPEST"
     polyA_signal    1965..2086
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(2505..3093)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /label="ori"
     CDS             complement(3293..4153)
                     /codon_start=1
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ccffcc"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     CDS             141..1790
                     /codon_start=1
                     /note="synthetic luc2 version of the luciferase gene"
                     /note="color: #ffcc00"
                     /product="firefly luciferase"
                     /transl_table=1
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
                     /label="luciferase"
ORIGIN
        1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcaagatc tggcctcggc
       61 ggccaagctt agacactaga gggtatataa tggaagctcg acttccagct tggcaatccg
      121 gtactgttgg taaagccacc atggaagatg ccaaaaacat taagaagggc ccagcgccat
      181 tctacccact cgaagacggg accgccggcg agcagctgca caaagccatg aagcgctacg
      241 ccctggtgcc cggcaccatc gcctttaccg acgcacatat cgaggtggac attacctacg
      301 ccgagtactt cgagatgagc gttcggctgg cagaagctat gaagcgctat gggctgaata
      361 caaaccatcg gatcgtggtg tgcagcgaga atagcttgca gttcttcatg cccgtgttgg
      421 gtgccctgtt catcggtgtg gctgtggccc cagctaacga catctacaac gagcgcgagc
      481 tgctgaacag catgggcatc agccagccca ccgtcgtatt cgtgagcaag aaagggctgc
      541 aaaagatcct caacgtgcaa aagaagctac cgatcataca aaagatcatc atcatggata
      601 gcaagaccga ctaccagggc ttccaaagca tgtacacctt cgtgacttcc catttgccac
      661 ccggcttcaa cgagtacgac ttcgtgcccg agagcttcga ccgggacaaa accatcgccc
      721 tgatcatgaa cagtagtggc agtaccggat tgcccaaggg cgtagcccta ccgcaccgca
      781 ccgcttgtgt ccgattcagt catgcccgcg accccatctt cggcaaccag atcatccccg
      841 acaccgctat cctcagcgtg gtgccatttc accacggctt cggcatgttc accacgctgg
      901 gctacttgat ctgcggcttt cgggtcgtgc tcatgtaccg cttcgaggag gagctattct
      961 tgcgcagctt gcaagactat aagattcaat ctgccctgct ggtgcccaca ctatttagct
     1021 tcttcgctaa gagcactctc atcgacaagt acgacctaag caacttgcac gagatcgcca
     1081 gcggcggggc gccgctcagc aaggaggtag gtgaggccgt ggccaaacgc ttccacctac
     1141 caggcatccg ccagggctac ggcctgacag aaacaaccag cgccattctg atcacccccg
     1201 aaggggacga caagcctggc gcagtaggca aggtggtgcc cttcttcgag gctaaggtgg
     1261 tggacttgga caccggtaag acactgggtg tgaaccagcg cggcgagctg tgcgtccgtg
     1321 gccccatgat catgagcggc tacgttaaca accccgaggc tacaaacgct ctcatcgaca
     1381 aggacggctg gctgcacagc ggcgacatcg cctactggga cgaggacgag cacttcttca
     1441 tcgtggaccg gctgaagagc ctgatcaaat acaagggcta ccaggtagcc ccagccgaac
     1501 tggagagcat cctgctgcaa caccccaaca tcttcgacgc cggggtcgcc ggcctgcccg
     1561 acgacgatgc cggcgagctg cccgccgcag tcgtcgtgct ggaacacggt aaaaccatga
     1621 ccgagaagga gatcgtggac tatgtggcca gccaggttac aaccgccaag aagctgcgcg
     1681 gtggtgttgt gttcgtggac gaggtgccta aaggactgac cggcaagttg gacgcccgca
     1741 agatccgcga gattctcatt aaggccaaga agggcggcaa gatcgccgtg aattctcacg
     1801 gcttccctcc cgaggtggag gagcaggccg ccggcaccct gcccatgagc tgcgcccagg
     1861 agagcggcat ggatagacac cctgctgctt gcgccagcgc caggatcaac gtctaaggcc
     1921 gcgactctag agtcggggcg gccggccgct tcgagcagac atgataagat acattgatga
     1981 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
     2041 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
     2101 cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa
     2161 cctctacaaa tgtggtaaaa tcgataagga tccgtcgacc gatgcccttg agagccttca
     2221 acccagtcag ctccttccgg tgggcgcggg gcatgactat cgtcgccgca cttatgactg
     2281 tcttctttat catgcaactc gtaggacagg tgccggcagc gctcttccgc ttcctcgctc
     2341 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     2401 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     2461 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     2521 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     2581 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     2641 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     2701 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     2761 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     2821 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     2881 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     2941 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     3001 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     3061 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     3121 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     3181 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     3241 tatgagtaaa cttggtctga cagcggccgc aaatgctaaa ccactgcagt ggttaccagt
     3301 gcttgatcag tgaggcaccg atctcagcga tctgcctatt tcgttcgtcc atagtggcct
     3361 gactccccgt cgtgtagatc actacgattc gtgagggctt accatcaggc cccagcgcag
     3421 caatgatgcc gcgagagccg cgttcaccgg cccccgattt gtcagcaatg aaccagccag
     3481 cagggagggc cgagcgaaga agtggtcctg ctactttgtc cgcctccatc cagtctatga
     3541 gctgctgtcg tgatgctaga gtaagaagtt cgccagtgag tagtttccga agagttgtgg
     3601 ccattgctac tggcatcgtg gtatcacgct cgtcgttcgg tatggcttcg ttcaactctg
     3661 gttcccagcg gtcaagccgg gtcacatgat cacccatatt atgaagaaat gcagtcagct
     3721 ccttagggcc tccgatcgtt gtcagaagta agttggccgc ggtgttgtcg ctcatggtaa
     3781 tggcagcact acacaattct cttaccgtca tgccatccgt aagatgcttt tccgtgaccg
     3841 gcgagtactc aaccaagtcg ttttgtgagt agtgtatacg gcgaccaagc tgctcttgcc
     3901 cggcgtctat acgggacaac accgcgccac atagcagtac tttgaaagtg ctcatcatcg
     3961 ggaatcgttc ttcggggcgg aaagactcaa ggatcttgcc gctattgaga tccagttcga
     4021 tatagcccac tcttgcaccc agttgatctt cagcatcttt tactttcacc agcgtttcgg
     4081 ggtgtgcaaa aacaggcaag caaaatgccg caaagaaggg aatgagtgcg acacgaaaat
     4141 gttggatgct catactcgtc ctttttcaat attattgaag catttatcag ggttactagt
     4201 acgtctctca aggataagta agtaatatta aggtacggga ggtattggac aggccgcaat
     4261 aaaatatctt tattttcatt acatctgtgt gttggttttt tgtgtgaatc gatagtacta
     4321 acatacgctc tccatcaaaa caaaacgaaa caaaacaaac tagcaaaata ggctgtcccc
     4381 agtgcaagtg caggtgccag aacatttctc t
//
