LOCUS . 5583 bp DNA circular UNK 01-JAN-1980 DEFINITION . ACCESSION VERSION KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers promoter 2290..2647 /note="SV40 enhancer and early promoter" /note="color: #ffffff" /label="SV40 promoter" CDS 2678..3277 /codon_start=1 /note="confers resistance to puromycin" /note="color: #ccffcc" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" CDS 1756..1803 /codon_start=1 /note="human codon-optimized" /note="color: #cc99b2" /product="non-ORF yeast peptide conferring ubiquitin-dependent degradation" /transl_table=1 /translation="ACKNWFSSLSHFVIHL" /label="hCL1" polyA_signal 5430..5478 /note="synthetic polyadenylation signal" /note="color: #a6acb3" /label="poly(A) signal" polyA_signal 3302..3350 /note="synthetic polyadenylation signal" /note="color: #a6acb3" /label="poly(A) signal" misc_feature 1..70 /note="multiple cloning site" /note="color: #99ccff" /label="MCS" misc_feature 5492..5583 /note="RNA polymerase II transcriptional pause signal from the human α2 globin gene" /note="color: #a6acb3" /label="pause site" CDS 1807..1926 /codon_start=1 /note="human codon-optimized" /note="color: #cc99b2" /product="PEST degradation sequence from mouse ornithine decarboxylase" /transl_table=1 /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV" /label="hPEST" polyA_signal 1975..2096 /note="SV40 polyadenylation signal" /note="color: #a6acb3" /label="SV40 poly(A) signal" rep_origin complement(3677..4265) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /note="color: #ffff00" /label="ori" CDS complement(4465..5325) /codon_start=1 /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /note="color: #ccffcc" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" CDS 100..1749 /codon_start=1 /note="synthetic luc2 version of the luciferase gene" /note="color: #ffcc00" /product="firefly luciferase" /transl_table=1 /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV" /label="luciferase" ORIGIN 1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcaagatc tggcctcggc 61 ggccaagctt ggcaatccgg tactgttggt aaagccacca tggaagatgc caaaaacatt 121 aagaagggcc cagcgccatt ctacccactc gaagacggga ccgccggcga gcagctgcac 181 aaagccatga agcgctacgc cctggtgccc ggcaccatcg cctttaccga cgcacatatc 241 gaggtggaca ttacctacgc cgagtacttc gagatgagcg ttcggctggc agaagctatg 301 aagcgctatg ggctgaatac aaaccatcgg atcgtggtgt gcagcgagaa tagcttgcag 361 ttcttcatgc ccgtgttggg tgccctgttc atcggtgtgg ctgtggcccc agctaacgac 421 atctacaacg agcgcgagct gctgaacagc atgggcatca gccagcccac cgtcgtattc 481 gtgagcaaga aagggctgca aaagatcctc aacgtgcaaa agaagctacc gatcatacaa 541 aagatcatca tcatggatag caagaccgac taccagggct tccaaagcat gtacaccttc 601 gtgacttccc atttgccacc cggcttcaac gagtacgact tcgtgcccga gagcttcgac 661 cgggacaaaa ccatcgccct gatcatgaac agtagtggca gtaccggatt gcccaagggc 721 gtagccctac cgcaccgcac cgcttgtgtc cgattcagtc atgcccgcga ccccatcttc 781 ggcaaccaga tcatccccga caccgctatc ctcagcgtgg tgccatttca ccacggcttc 841 ggcatgttca ccacgctggg ctacttgatc tgcggctttc gggtcgtgct catgtaccgc 901 ttcgaggagg agctattctt gcgcagcttg caagactata agattcaatc tgccctgctg 961 gtgcccacac tatttagctt cttcgctaag agcactctca tcgacaagta cgacctaagc 1021 aacttgcacg agatcgccag cggcggggcg ccgctcagca aggaggtagg tgaggccgtg 1081 gccaaacgct tccacctacc aggcatccgc cagggctacg gcctgacaga aacaaccagc 1141 gccattctga tcacccccga aggggacgac aagcctggcg cagtaggcaa ggtggtgccc 1201 ttcttcgagg ctaaggtggt ggacttggac accggtaaga cactgggtgt gaaccagcgc 1261 ggcgagctgt gcgtccgtgg ccccatgatc atgagcggct acgttaacaa ccccgaggct 1321 acaaacgctc tcatcgacaa ggacggctgg ctgcacagcg gcgacatcgc ctactgggac 1381 gaggacgagc acttcttcat cgtggaccgg ctgaagagcc tgatcaaata caagggctac 1441 caggtagccc cagccgaact ggagagcatc ctgctgcaac accccaacat cttcgacgcc 1501 ggggtcgccg gcctgcccga cgacgatgcc ggcgagctgc ccgccgcagt cgtcgtgctg 1561 gaacacggta aaaccatgac cgagaaggag atcgtggact atgtggccag ccaggttaca 1621 accgccaaga agctgcgcgg tggtgttgtg ttcgtggacg aggtgcctaa aggactgacc 1681 ggcaagttgg acgcccgcaa gatccgcgag attctcatta aggccaagaa gggcggcaag 1741 atcgccgtga attctgcttg caagaactgg ttcagtagct taagccactt tgtgatccac 1801 cttaacagcc acggcttccc tcccgaggtg gaggagcagg ccgccggcac cctgcccatg 1861 agctgcgccc aggagagcgg catggataga caccctgctg cttgcgccag cgccaggatc 1921 aacgtctaac tgcagtctag agtcggggcg gccggccgct tcgagcagac atgataagat 1981 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg 2041 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca 2101 acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa 2161 gcaagtaaaa cctctacaaa tgtggtaaaa tcgataagga tccgtttgcg tattgggcgc 2221 tcttccgctg atctgcgcag caccatggcc tgaaataacc tctgaaagag gaacttggtt 2281 agctaccttc tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa 2341 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac 2401 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa 2461 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag 2521 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc 2581 cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt 2641 ttgcaaaaag ctcgattctt ctgacactag cgccaccatg accgagtaca agcctaccgt 2701 gcgcctggcc actcgcgatg atgtgccccg cgccgtccgc actctggccg ccgctttcgc 2761 cgactacccc gctacccggc acaccgtgga ccccgaccgg cacatcgagc gtgtgacaga 2821 gttgcaggag ctgttcctga cccgcgtcgg gctggacatc ggcaaggtgt gggtagccga 2881 cgacggcgcg gccgtggccg tgtggactac ccccgagagc gttgaggccg gcgccgtgtt 2941 cgccgagatc ggcccccgaa tggccgagct gagcggcagc cgcctggccg cccagcagca 3001 aatggagggc ctgcttgccc cccatcgtcc caaggagcct gcctggtttc tggccactgt 3061 aggagtgagc cccgaccacc agggcaaggg cttgggcagc gccgtcgtgt tgcccggcgt 3121 agaggccgcc gaacgcgccg gtgtgcccgc ctttctcgaa acaagcgcac caagaaacct 3181 tccattctac gagcgcctgg gcttcaccgt gaccgccgat gtcgaggtgc ccgagggacc 3241 taggacctgg tgtatgacac gaaaacctgg cgcctaatga tctagaaccg gtcatggccg 3301 caataaaata tctttatttt cattacatct gtgtgttggt tttttgtgtg ttcgaactag 3361 atgctgtcga ccgatgccct tgagagcctt caacccagtc agctccttcc ggtgggcgcg 3421 gggcatgact atcgtcgccg cacttatgac tgtcttcttt atcatgcaac tcgtaggaca 3481 ggtgccggca gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 3541 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg 3601 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg 3661 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 3721 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 3781 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 3841 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg 3901 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 3961 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 4021 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 4081 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc 4141 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 4201 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 4261 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 4321 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt 4381 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagcggcc 4441 gcaaatgcta aaccactgca gtggttacca gtgcttgatc agtgaggcac cgatctcagc 4501 gatctgccta tttcgttcgt ccatagtggc ctgactcccc gtcgtgtaga tcactacgat 4561 tcgtgagggc ttaccatcag gccccagcgc agcaatgatg ccgcgagagc cgcgttcacc 4621 ggcccccgat ttgtcagcaa tgaaccagcc agcagggagg gccgagcgaa gaagtggtcc 4681 tgctactttg tccgcctcca tccagtctat gagctgctgt cgtgatgcta gagtaagaag 4741 ttcgccagtg agtagtttcc gaagagttgt ggccattgct actggcatcg tggtatcacg 4801 ctcgtcgttc ggtatggctt cgttcaactc tggttcccag cggtcaagcc gggtcacatg 4861 atcacccata ttatgaagaa atgcagtcag ctccttaggg cctccgatcg ttgtcagaag 4921 taagttggcc gcggtgttgt cgctcatggt aatggcagca ctacacaatt ctcttaccgt 4981 catgccatcc gtaagatgct tttccgtgac cggcgagtac tcaaccaagt cgttttgtga 5041 gtagtgtata cggcgaccaa gctgctcttg cccggcgtct atacgggaca acaccgcgcc 5101 acatagcagt actttgaaag tgctcatcat cgggaatcgt tcttcggggc ggaaagactc 5161 aaggatcttg ccgctattga gatccagttc gatatagccc actcttgcac ccagttgatc 5221 ttcagcatct tttactttca ccagcgtttc ggggtgtgca aaaacaggca agcaaaatgc 5281 cgcaaagaag ggaatgagtg cgacacgaaa atgttggatg ctcatactcg tcctttttca 5341 atattattga agcatttatc agggttacta gtacgtctct caaggataag taagtaatat 5401 taaggtacgg gaggtattgg acaggccgca ataaaatatc tttattttca ttacatctgt 5461 gtgttggttt tttgtgtgaa tcgatagtac taacatacgc tctccatcaa aacaaaacga 5521 aacaaaacaa actagcaaaa taggctgtcc ccagtgcaag tgcaggtgcc agaacatttc 5581 tct //