LOCUS       .                       5969 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             100..1749
                     /codon_start=1
                     /note="synthetic luc2 version of the luciferase gene"
                     /note="color: #ffcc00"
                     /product="firefly luciferase"
                     /transl_table=1
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
                     /label="luciferase"
     CDS             complement(4851..5711)
                     /codon_start=1
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ccffcc"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      complement(4063..4651)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /label="ori"
     polyA_signal    1924..2045
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
                     /label="SV40 poly(A) signal"
     CDS             1753..1872
                     /codon_start=1
                     /note="human codon-optimized"
                     /note="color: #cc99b2"
                     /product="PEST degradation sequence from mouse ornithine
                     decarboxylase"
                     /transl_table=1
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
                     /label="hPEST"
     misc_feature    5878..5969
                     /note="RNA polymerase II transcriptional pause signal from
                     the human α2 globin gene"
                     /note="color: #a6acb3"
                     /label="pause site"
     misc_feature    1..70
                     /note="multiple cloning site"
                     /note="color: #99ccff"
                     /label="MCS"
     polyA_signal    3688..3736
                     /note="synthetic polyadenylation signal"
                     /note="color: #a6acb3"
                     /label="poly(A) signal"
     polyA_signal    5816..5864
                     /note="synthetic polyadenylation signal"
                     /note="color: #a6acb3"
                     /label="poly(A) signal"
     CDS             2627..3664
                     /codon_start=1
                     /note="confers resistance to hygromycin"
                     /note="color: #ccffcc"
                     /product="hygromycin B phosphotransferase"
                     /transl_table=1
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     EVGRV*"
                     /label="HygR"
     promoter        2239..2596
                     /note="SV40 enhancer and early promoter"
                     /note="color: #ffffff"
                     /label="SV40 promoter"
ORIGIN
        1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcaagatc tggcctcggc
       61 ggccaagctt ggcaatccgg tactgttggt aaagccacca tggaagatgc caaaaacatt
      121 aagaagggcc cagcgccatt ctacccactc gaagacggga ccgccggcga gcagctgcac
      181 aaagccatga agcgctacgc cctggtgccc ggcaccatcg cctttaccga cgcacatatc
      241 gaggtggaca ttacctacgc cgagtacttc gagatgagcg ttcggctggc agaagctatg
      301 aagcgctatg ggctgaatac aaaccatcgg atcgtggtgt gcagcgagaa tagcttgcag
      361 ttcttcatgc ccgtgttggg tgccctgttc atcggtgtgg ctgtggcccc agctaacgac
      421 atctacaacg agcgcgagct gctgaacagc atgggcatca gccagcccac cgtcgtattc
      481 gtgagcaaga aagggctgca aaagatcctc aacgtgcaaa agaagctacc gatcatacaa
      541 aagatcatca tcatggatag caagaccgac taccagggct tccaaagcat gtacaccttc
      601 gtgacttccc atttgccacc cggcttcaac gagtacgact tcgtgcccga gagcttcgac
      661 cgggacaaaa ccatcgccct gatcatgaac agtagtggca gtaccggatt gcccaagggc
      721 gtagccctac cgcaccgcac cgcttgtgtc cgattcagtc atgcccgcga ccccatcttc
      781 ggcaaccaga tcatccccga caccgctatc ctcagcgtgg tgccatttca ccacggcttc
      841 ggcatgttca ccacgctggg ctacttgatc tgcggctttc gggtcgtgct catgtaccgc
      901 ttcgaggagg agctattctt gcgcagcttg caagactata agattcaatc tgccctgctg
      961 gtgcccacac tatttagctt cttcgctaag agcactctca tcgacaagta cgacctaagc
     1021 aacttgcacg agatcgccag cggcggggcg ccgctcagca aggaggtagg tgaggccgtg
     1081 gccaaacgct tccacctacc aggcatccgc cagggctacg gcctgacaga aacaaccagc
     1141 gccattctga tcacccccga aggggacgac aagcctggcg cagtaggcaa ggtggtgccc
     1201 ttcttcgagg ctaaggtggt ggacttggac accggtaaga cactgggtgt gaaccagcgc
     1261 ggcgagctgt gcgtccgtgg ccccatgatc atgagcggct acgttaacaa ccccgaggct
     1321 acaaacgctc tcatcgacaa ggacggctgg ctgcacagcg gcgacatcgc ctactgggac
     1381 gaggacgagc acttcttcat cgtggaccgg ctgaagagcc tgatcaaata caagggctac
     1441 caggtagccc cagccgaact ggagagcatc ctgctgcaac accccaacat cttcgacgcc
     1501 ggggtcgccg gcctgcccga cgacgatgcc ggcgagctgc ccgccgcagt cgtcgtgctg
     1561 gaacacggta aaaccatgac cgagaaggag atcgtggact atgtggccag ccaggttaca
     1621 accgccaaga agctgcgcgg tggtgttgtg ttcgtggacg aggtgcctaa aggactgacc
     1681 ggcaagttgg acgcccgcaa gatccgcgag attctcatta aggccaagaa gggcggcaag
     1741 atcgccgtga attctcacgg cttccctccc gaggtggagg agcaggccgc cggcaccctg
     1801 cccatgagct gcgcccagga gagcggcatg gatagacacc ctgctgcttg cgccagcgcc
     1861 aggatcaacg tctaaggccg cgactctaga gtcggggcgg ccggccgctt cgagcagaca
     1921 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
     1981 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
     2041 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg
     2101 ttttttaaag caagtaaaac ctctacaaat gtggtaaaat cgataaggat ccgtttgcgt
     2161 attgggcgct cttccgctga tctgcgcagc accatggcct gaaataacct ctgaaagagg
     2221 aacttggtta gctaccttct gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg
     2281 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     2341 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     2401 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     2461 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     2521 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     2581 cctaggcttt tgcaaaaagc tcgattcttc tgacactagc gccaccatga agaagcccga
     2641 actcaccgct accagcgttg aaaaatttct catcgagaag ttcgacagtg tgagcgacct
     2701 gatgcagttg tcggagggcg aagagagccg agccttcagc ttcgatgtcg gcggacgcgg
     2761 ctatgtactg cgggtgaata gctgcgctga tggcttctac aaagaccgct acgtgtaccg
     2821 ccacttcgcc agcgctgcac tacccatccc cgaagtgttg gacatcggcg agttcagcga
     2881 gagcctgaca tactgcatca gtagacgcgc ccaaggcgtt actctccaag acctccccga
     2941 aacagagctg cctgctgtgt tacagcctgt cgccgaagct atggatgcta ttgccgccgc
     3001 cgacctcagt caaaccagcg gcttcggccc attcgggccc caaggcatcg gccagtacac
     3061 aacctggcgg gatttcattt gcgccattgc tgatccccat gtctaccact ggcagaccgt
     3121 gatggacgac accgtgtccg ccagcgtagc tcaagccctg gacgaactga tgctgtgggc
     3181 cgaagactgt cccgaggtgc gccacctcgt ccatgccgac ttcggcagca acaacgtcct
     3241 gaccgacaac ggccgcatca ccgccgtaat cgactggtcc gaagctatgt tcggggacag
     3301 tcagtacgag gtggccaaca tcttcttctg gcggccctgg ctggcttgca tggagcagca
     3361 gactcgctac ttcgagcgcc ggcatcccga gctggccggc agccctcgtc tgcgagccta
     3421 catgctgcgc atcggcctgg atcagctcta ccagagcctc gtggacggca acttcgacga
     3481 tgctgcctgg gctcaaggcc gctgcgatgc catcgtccgc agcggggccg gcaccgtcgg
     3541 tcgcacacaa atcgctcgcc ggagcgcagc cgtatggacc gacggctgcg tcgaggtgct
     3601 ggccgacagc ggcaaccgcc ggcccagtac acgaccgcgc gctaaggagg taggtcgagt
     3661 ttaaactcta gaaccggtca tggccgcaat aaaatatctt tattttcatt acatctgtgt
     3721 gttggttttt tgtgtgttcg aactagatgc tgtcgaccga tgcccttgag agccttcaac
     3781 ccagtcagct ccttccggtg ggcgcggggc atgactatcg tcgccgcact tatgactgtc
     3841 ttctttatca tgcaactcgt aggacaggtg ccggcagcgc tcttccgctt cctcgctcac
     3901 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     3961 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     4021 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     4081 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     4141 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     4201 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     4261 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     4321 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     4381 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     4441 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     4501 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     4561 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     4621 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     4681 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     4741 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     4801 tgagtaaact tggtctgaca gcggccgcaa atgctaaacc actgcagtgg ttaccagtgc
     4861 ttgatcagtg aggcaccgat ctcagcgatc tgcctatttc gttcgtccat agtggcctga
     4921 ctccccgtcg tgtagatcac tacgattcgt gagggcttac catcaggccc cagcgcagca
     4981 atgatgccgc gagagccgcg ttcaccggcc cccgatttgt cagcaatgaa ccagccagca
     5041 gggagggccg agcgaagaag tggtcctgct actttgtccg cctccatcca gtctatgagc
     5101 tgctgtcgtg atgctagagt aagaagttcg ccagtgagta gtttccgaag agttgtggcc
     5161 attgctactg gcatcgtggt atcacgctcg tcgttcggta tggcttcgtt caactctggt
     5221 tcccagcggt caagccgggt cacatgatca cccatattat gaagaaatgc agtcagctcc
     5281 ttagggcctc cgatcgttgt cagaagtaag ttggccgcgg tgttgtcgct catggtaatg
     5341 gcagcactac acaattctct taccgtcatg ccatccgtaa gatgcttttc cgtgaccggc
     5401 gagtactcaa ccaagtcgtt ttgtgagtag tgtatacggc gaccaagctg ctcttgcccg
     5461 gcgtctatac gggacaacac cgcgccacat agcagtactt tgaaagtgct catcatcggg
     5521 aatcgttctt cggggcggaa agactcaagg atcttgccgc tattgagatc cagttcgata
     5581 tagcccactc ttgcacccag ttgatcttca gcatctttta ctttcaccag cgtttcgggg
     5641 tgtgcaaaaa caggcaagca aaatgccgca aagaagggaa tgagtgcgac acgaaaatgt
     5701 tggatgctca tactcgtcct ttttcaatat tattgaagca tttatcaggg ttactagtac
     5761 gtctctcaag gataagtaag taatattaag gtacgggagg tattggacag gccgcaataa
     5821 aatatcttta ttttcattac atctgtgtgt tggttttttg tgtgaatcga tagtactaac
     5881 atacgctctc catcaaaaca aaacgaaaca aaacaaacta gcaaaatagg ctgtccccag
     5941 tgcaagtgca ggtgccagaa catttctct
//
