LOCUS       .                       3000 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    10..113
                     /note="multiple cloning site"
                     /note="color: #99ccff"
                     /label="MCS"
     CDS             complement(1322..2182)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ccffcc"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      complement(563..1151)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /label="ori"
     rep_origin      complement(2365..2820)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
                     /label="f1 ori"
     promoter        complement(2183..2287)
                     /gene="bla"
                     /label="AmpR promoter"
                     /note="color: #ffffff"
     promoter        complement(209..239)
                     /note="promoter for the E. coli lac operon"
                     /note="color: #ffffff"
                     /label="lac promoter"
     promoter        complement(125..143)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /note="color: #ffffff"
                     /label="SP6 promoter"
     promoter        2..2984
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff"
                     /label="T7 promoter"
     primer_bind     complement(161..177)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /note="color: #a020f0"
                     /label="M13 rev"
     protein_bind    185..201
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
                     /label="lac operator"
     primer_bind     2961..2977
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /note="color: #a020f0"
                     /label="M13 fwd"
     CDS             complement(165..2797)
                     /codon_start=1
                     /gene="lacZ (fragment)"
                     /product="LacZα fragment of β-galactosidase"
                     /transl_table=1
                     /translation="MTMITPSYLGDTIEYSSYASNALGALPYGRPAGGRTSDIPRPWRP
                     GACDVGPNSPYSESYYNSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRP
                     SQQLRSLNGEWTRPVAAH*"
                     /label="lacZα"
                     /note="color: #993366"
ORIGIN
        1 gggcgaattg ggcccgacgt cgcatgctcc cggccgccat ggccgcggga tatcactagt
       61 gcggccgcct gcaggtcgac catatgggag agctcccaac gcgttggatg catagcttga
      121 gtattctata gtgtcaccta aatagcttgg cgtaatcatg gtcatagctg tttcctgtgt
      181 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
      241 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
      301 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
      361 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
      421 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
      481 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
      541 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
      601 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
      661 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
      721 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
      781 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
      841 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
      901 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
      961 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     1021 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     1081 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     1141 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     1201 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     1261 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     1321 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     1381 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     1441 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     1501 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     1561 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     1621 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     1681 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     1741 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     1801 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     1861 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     1921 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     1981 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     2041 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     2101 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     2161 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     2221 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     2281 ttccgcgcac atttccccga aaagtgccac ctgatgcggt gtgaaatacc gcacagatgc
     2341 gtaaggagaa aataccgcat caggaaattg taagcgttaa tattttgtta aaattcgcgt
     2401 taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt
     2461 ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc
     2521 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg
     2581 gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac
     2641 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg
     2701 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag
     2761 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt
     2821 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
     2881 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
     2941 gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa ttgtaatacg actcactata
//
