LOCUS       .                       4339 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        complement(611..629)
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff"
                     /label="T7 promoter"
     terminator      404..451
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
                     /note="color: #ffffff"
                     /label="T7 terminator"
     CDS             2190..2381
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein"
                     /transl_table=1
                     /translation="VTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL*"
                     /label="rop"
                     /note="color: #993366"
     rep_origin      complement(2811..3399)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /label="ori"
     CDS             3521..4336
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /note="confers resistance to kanamycin"
                     /note="color: #ccffcc"
                     /product="aminoglycoside phosphotransferase"
                     /transl_table=1
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"
                     /label="KanR"
     CDS             complement(517..549)
                     /codon_start=1
                     /product="epitope tag from a T7 major capsid protein"
                     /transl_table=1
                     /translation="MASMTGGQQMG"
                     /label="T7 tag"
                     /note="color: #cc99b2"
     RBS             558..563
                     /note="ribosome binding site"
                     /note="color: #a6acb3"
                     /label="RBS"
ORIGIN
        1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
       61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
      121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
      181 gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
      241 tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
      301 ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
      361 cacacccgtc ctgtggatat ccggatatag ttcctccttt cagcaaaaaa cccctcaaga
      421 cccgtttaga ggccccaagg ggttatgcta gttattgctc agcggtggca gcagccaact
      481 cagcttcctt tcgggctttg ttagcagccg gatccgaccc atttgctgtc caccagtcat
      541 gctagccata tgtatatctc cttcttaaag ttaaacaaaa ttatttctag agggaaaccg
      601 ttgtggtctc cctatagtga gtcgtattaa tttcgcggga tcgagatctc gatcctctac
      661 gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg cgcctatatc
      721 gccgacatca ccgatgggga agatcgggct cgccacttcg ggctcatgag cgcttgtttc
      781 ggcgtgggta tggtggcagg ccccgtggcc gggggactgt tgggcgccat ctccttgcat
      841 gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc tactactggg ctgcttccta
      901 atgcaggagt cgcataaggg agagcgtcga ccgatgccct tgagagcctt caacccagtc
      961 agctccttcc ggtgggcgcg gggcatgact atcgtcgccg cacttatgac tgtcttcttt
     1021 atcatgcaac tcgtaggaca ggtgccggca gcgctctggg tcattttcgg cgaggaccgc
     1081 tttcgctgga gcgcgacgat gatcggcctg tcgcttgcgg tattcggaat cttgcacgcc
     1141 ctcgctcaag ccttcgtcac tggtcccgcc accaaacgtt tcggcgagaa gcaggccatt
     1201 atcgccggca tggcggccga cgcgctgggc tacgtcttgc tggcgttcgc gacgcgaggc
     1261 tggatggcct tccccattat gattcttctc gcttccggcg gcatcgggat gcccgcgttg
     1321 caggccatgc tgtccaggca ggtagatgac gaccatcagg gacagcttca aggatcgctc
     1381 gcggctctta ccagcctaac ttcgatcact ggaccgctga tcgtcacggc gatttatgcc
     1441 gcctcggcga gcacatggaa cgggttggca tggattgtag gcgccgccct ataccttgtc
     1501 tgcctccccg cgttgcgtcg cggtgcatgg agccgggcca cctcgacctg aatggaagcc
     1561 ggcggcacct cgctaacgga ttcaccactc caagaattgg agccaatcaa ttcttgcgga
     1621 gaactgtgaa tgcgcaaacc aacccttggc agaacatatc catcgcgtcc gccatctcca
     1681 gcagccgcac gcggcgcatc tcgggcagcg ttgggtcctg gccacgggtg cgcatgatcg
     1741 tgctcctgtc gttgaggacc cggctaggct ggcggggttg ccttactggt tagcagaatg
     1801 aatcaccgat acgcgagcga acgtgaagcg actgctgctg caaaacgtct gcgacctgag
     1861 caacaacatg aatggtcttc ggtttccgtg tttcgtaaag tctggaaacg cggaagtcag
     1921 cgccctgcac cattatgttc cggatctgca tcgcaggatg ctgctggcta ccctgtggaa
     1981 cacctacatc tgtattaacg aagcgctggc attgaccctg agtgattttt ctctggtccc
     2041 gccgcatcca taccgccagt tgtttaccct cacaacgttc cagtaaccgg gcatgttcat
     2101 catcagtaac ccgtatcgtg agcatcctct ctcgtttcat cggtatcatt acccccatga
     2161 acagaaatcc cccttacacg gaggcatcag tgaccaaaca ggaaaaaacc gcccttaaca
     2221 tggcccgctt tatcagaagc cagacattaa cgcttctgga gaaactcaac gagctggacg
     2281 cggatgaaca ggcagacatc tgtgaatcgc ttcacgacca cgctgatgag ctttaccgca
     2341 gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
     2401 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     2461 cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt
     2521 atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatatgcgg
     2581 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc
     2641 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     2701 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     2761 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     2821 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     2881 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     2941 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     3001 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     3061 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     3121 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     3181 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     3241 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     3301 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     3361 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     3421 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgaacaat
     3481 aaaactgtct gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga
     3541 aacgtcttgc tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa
     3601 atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc tatcgattgt atgggaagcc
     3661 cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga
     3721 tgagatggtc agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt
     3781 tatccgtact cctgatgatg catggttact caccactgcg atccccggga aaacagcatt
     3841 ccaggtatta gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt
     3901 cctgcgccgg ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt
     3961 tcgtctcgct caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga
     4021 tgacgagcgt aatggctggc ctgttgaaca agtctggaaa gaaatgcata agcttttgcc
     4081 attctcaccg gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga
     4141 cgaggggaaa ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca
     4201 ggatcttgcc atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct
     4261 ttttcaaaaa tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct
     4321 cgatgagttt ttctaagaa
//
