LOCUS       .                       3306 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    141..228
                     /note="multiple cloning site"
                     /note="color: #99ccff"
                     /label="MCS"
     promoter        complement(331..349)
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff"
                     /label="T7 promoter"
     terminator      28..75
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
                     /note="color: #ffffff"
                     /label="T7 terminator"
     promoter        complement(3099..3203)
                     /gene="bla"
                     /label="AmpR promoter"
                     /note="color: #ffffff"
     CDS             858..1049
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein"
                     /transl_table=1
                     /translation="VTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL*"
                     /label="rop"
                     /note="color: #993366"
     rep_origin      complement(1479..2067)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /label="ori"
     CDS             complement(2238..3098)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ccffcc"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     CDS             complement(237..269)
                     /codon_start=1
                     /product="epitope tag from a T7 major capsid protein"
                     /transl_table=1
                     /translation="MASMTGGQQMG"
                     /label="T7 tag"
                     /note="color: #cc99b2"
     RBS             278..283
                     /note="ribosome binding site"
                     /note="color: #a6acb3"
                     /label="RBS"
ORIGIN
        1 ttatccggat atagttcctc ctttcagcaa aaaacccctc aagacccgtt tagaggcccc
       61 aaggggttat gctagttatt gctcagcggt ggcagcagcc aactcagctt cctttcgggc
      121 tttgttagca gccggatctg ctcgagcggc cgccagtgtg atggatatct gcagaattcc
      181 agcacactgg cggccgttac tagtggatcc gagctcggta ccaagcttga atcccgaccc
      241 atttgctgtc caccagtcat gctagccata tgtatatctc cttcttaaag ttaaacaaaa
      301 ttatttctag agggaaaccg ttgtggtctc cctatagtga gtcgtattaa tttcgcggga
      361 tcgagatctc gggcagcgtt gggtcctggc cacgggtgcg catgatcgtg ctcctgtcgt
      421 tgaggacccg gctaggctgg cggggttgcc ttactggtta gcagaatgaa tcaccgatac
      481 gcgagcgaac gtgaagcgac tgctgctgca aaacgtctgc gacctgagca acaacatgaa
      541 tggtcttcgg tttccgtgtt tcgtaaagtc tggaaacgcg gaagtcagcg ccctgcacca
      601 ttatgttccg gatctgcatc gcaggatgct gctggctacc ctgtggaaca cctacatctg
      661 tattaacgaa gcgctggcat tgaccctgag tgatttttct ctggtcccgc cgcatccata
      721 ccgccagttg tttaccctca caacgttcca gtaaccgggc atgttcatca tcagtaaccc
      781 gtatcgtgag catcctctct cgtttcatcg gtatcattac ccccatgaac agaaatcccc
      841 cttacacgga ggcatcagtg accaaacagg aaaaaaccgc ccttaacatg gcccgcttta
      901 tcagaagcca gacattaacg cttctggaga aactcaacga gctggacgcg gatgaacagg
      961 cagacatctg tgaatcgctt cacgaccacg ctgatgagct ttaccgcagc tgcctcgcgc
     1021 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     1081 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     1141 ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat actggcttaa
     1201 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atatgcggtg tgaaataccg
     1261 cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac
     1321 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     1381 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     1441 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     1501 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     1561 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     1621 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     1681 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     1741 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     1801 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     1861 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     1921 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     1981 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     2041 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     2101 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     2161 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     2221 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     2281 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     2341 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     2401 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     2461 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     2521 gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg
     2581 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     2641 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     2701 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     2761 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     2821 atgcggcgac cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc
     2881 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     2941 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     3001 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     3061 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     3121 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     3181 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
     3241 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctt
     3301 caagaa
//
