LOCUS       .                       6525 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    2830..2927
                     /note="multiple cloning site 2"
                     /note="color: #99ccff"
                     /label="MCS 2"
     misc_feature    2074..2160
                     /note="multiple cloning site 1"
                     /note="color: #99ccff"
                     /label="MCS 1"
     terminator      1779..1944
                     /gene="S. cerevisiae ADH1"
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
                     /note="color: #ffffff"
                     /label="ADH1 terminator"
     rep_origin      5115..6457
                     /note="yeast 2μ plasmid origin of replication"
                     /note="color: #ffff00"
                     /label="2μ ori"
     CDS             complement(4123..4983)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ccffcc"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     CDS             468..1142
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /note="yeast auxotrophic marker"
                     /note="color: #ff7f50"
                     /product="phosphoribosylanthranilate isomerase, required
                     for tryptophan biosynthesis"
                     /transl_table=1
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK*"
                     /label="TRP1"
     promoter        2162..2826
                     /gene="GAL1 and GAL10 genes of S. cerevisiae"
                     /note="divergent inducible promoter, regulated by Gal4"
                     /note="color: #ffffff"
                     /label="GAL1,10 promoter"
     rep_origin      complement(3364..3952)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /label="ori"
     rep_origin      complement(1241..1696)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
                     /label="f1 ori"
     promoter        187..467
                     /gene="S. cerevisiae TRP1"
                     /label="TRP1 promoter"
                     /note="color: #ffffff"
     terminator      2932..3121
                     /gene="S. cerevisiae CYC1"
                     /note="transcription terminator for CYC1"
                     /note="color: #ffffff"
                     /label="CYC1 terminator"
     protein_bind    2378..2495
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating
                     Gal4-dependent induction"
                     /note="color: #31849b"
                     /label="UAS"
     promoter        complement(4984..5088)
                     /gene="bla"
                     /label="AmpR promoter"
                     /note="color: #ffffff"
     CDS             2873..2902
                     /codon_start=1
                     /product="Myc (human c-Myc oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
                     /note="color: #cc99b2"
     CDS             complement(2092..2115)
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
                     /note="color: #cc99b2"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataaacg acattactat atatataata taggaagcat ttaatagaca gcatcgtaat
      241 atatgtgtac tttgcagtta tgacgccaga tggcagtagt ggaagatatt ctttattgaa
      301 aaatagcttg tcaccttacg tacaatcttg atccggagct tttctttttt tgccgattaa
      361 gaattaattc ggtcgaaaaa agaaaaggag agggccaaga gggagggcat tggtgactat
      421 tgagcacgtg agtatacgtg attaagcaca caaaggcagc ttggagtatg tctgttatta
      481 atttcacagg tagttctggt ccattggtga aagtttgcgg cttgcagagc acagaggccg
      541 cagaatgtgc tctagattcc gatgctgact tgctgggtat tatatgtgtg cccaatagaa
      601 agagaacaat tgacccggtt attgcaagga aaatttcaag tcttgtaaaa gcatataaaa
      661 atagttcagg cactccgaaa tacttggttg gcgtgtttcg taatcaacct aaggaggatg
      721 ttttggctct ggtcaatgat tacggcattg atatcgtcca actgcatgga gatgagtcgt
      781 ggcaagaata ccaagagttc ctcggtttgc cagttattaa aagactcgta tttccaaaag
      841 actgcaacat actactcagt gcagcttcac agaaacctca ttcgtttatt cccttgtttg
      901 attcagaagc aggtgggaca ggtgaacttt tggattggaa ctcgatttct gactgggttg
      961 gaaggcaaga gagccccgaa agcttacatt ttatgttagc tggtggactg acgccagaaa
     1021 atgttggtga tgcgcttaga ttaaatggcg ttattggtgt tgatgtaagc ggaggtgtgg
     1081 agacaaatgg tgtaaaagac tctaacaaaa tagcaaattt cgtcaaaaat gctaagaaat
     1141 aggttattac tgagtagtat ttatttaagt attgtttgtg cacttgccta tgcggtgtga
     1201 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt
     1261 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
     1321 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
     1381 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
     1441 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg
     1501 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
     1561 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
     1621 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg
     1681 ccgctacagg gcgcgtcgcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
     1741 tcggtgcggg cctcttcgct attacgccag ctgaattgga gcgacctcat gctatacctg
     1801 agaaagcaac ctgacctaca ggaaagagtt actcaagaat aagaattttc gttttaaaac
     1861 ctaagagtca ctttaaaatt tgtatacact tatttttttt ataacttatt taataataaa
     1921 aatcataaat cataagaaat tcgcttattt agaagtgtca acaacgtatc taccaacgat
     1981 ttgacccttt tccatctttt cgtaaatttc tggcaaggta gacaagccga caaccttgat
     2041 tggagacttg accaaacctc tggcgaagaa ttgttaatta agagctcaga tcttatcgtc
     2101 gtcatccttg taatccatcg atactagtgc ggccgccctt tagtgagggt tgaattcgaa
     2161 ttttcaaaaa ttcttacttt ttttttggat ggacgcaaag aagtttaata atcatattac
     2221 atggcattac caccatatac atatccatat acatatccat atctaatctt acttatatgt
     2281 tgtggaaatg taaagagccc cattatctta gcctaaaaaa accttctctt tggaactttc
     2341 agtaatacgc ttaactgctc attgctatat tgaagtacgg attagaagcc gccgagcggg
     2401 tgacagccct ccgaaggaag actctcctcc gtgcgtcctc gtcttcaccg gtcgcgttcc
     2461 tgaaacgcag atgtgcctcg cgccgcactg ctccgaacaa taaagattct acaatactag
     2521 cttttatggt tatgaagagg aaaaattggc agtaacctgg ccccacaaac cttcaaatga
     2581 acgaatcaaa ttaacaacca taggatgata atgcgattag ttttttagcc ttatttctgg
     2641 ggtaattaat cagcgaagcg atgatttttg atctattaac agatatataa atgcaaaaac
     2701 tgcataacca ctttaactaa tactttcaac attttcggtt tgtattactt cttattcaaa
     2761 tgtaataaaa gtatcaacaa aaaattgtta atatacctct atactttaac gtcaaggaga
     2821 aaaaaccccg gatccgtaat acgactcact atagggcccg ggcgtcgaca tggaacagaa
     2881 gttgatttcc gaagaagacc tcgagtaagc ttggtaccgc ggctagctaa gatccgctct
     2941 aaccgaaaag gaaggagtta gacaacctga agtctaggtc cctatttatt tttttatagt
     3001 tatgttagta ttaagaacgt tatttatatt tcaaattttt cttttttttc tgtacagacg
     3061 cgtgtacgca tgtaacatta tactgaaaac cttgcttgag aaggttttgg gacgctcgaa
     3121 gatccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     3181 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     3241 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     3301 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     3361 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     3421 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     3481 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     3541 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     3601 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     3661 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     3721 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     3781 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
     3841 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     3901 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     3961 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     4021 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     4081 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     4141 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     4201 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     4261 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     4321 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     4381 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     4441 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     4501 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     4561 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     4621 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     4681 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     4741 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     4801 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     4861 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     4921 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     4981 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     5041 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     5101 aaaagtgcca cctgaacgaa gcatctgtgc ttcattttgt agaacaaaaa tgcaacgcga
     5161 gagcgctaat ttttcaaaca aagaatctga gctgcatttt tacagaacag aaatgcaacg
     5221 cgaaagcgct attttaccaa cgaagaatct gtgcttcatt tttgtaaaac aaaaatgcaa
     5281 cgcgagagcg ctaatttttc aaacaaagaa tctgagctgc atttttacag aacagaaatg
     5341 caacgcgaga gcgctatttt accaacaaag aatctatact tcttttttgt tctacaaaaa
     5401 tgcatcccga gagcgctatt tttctaacaa agcatcttag attacttttt ttctcctttg
     5461 tgcgctctat aatgcagtct cttgataact ttttgcactg taggtccgtt aaggttagaa
     5521 gaaggctact ttggtgtcta ttttctcttc cataaaaaaa gcctgactcc acttcccgcg
     5581 tttactgatt actagcgaag ctgcgggtgc attttttcaa gataaaggca tccccgatta
     5641 tattctatac cgatgtggat tgcgcatact ttgtgaacag aaagtgatag cgttgatgat
     5701 tcttcattgg tcagaaaatt atgaacggtt tcttctattt tgtctctata tactacgtat
     5761 aggaaatgtt tacattttcg tattgttttc gattcactct atgaatagtt cttactacaa
     5821 tttttttgtc taaagagtaa tactagagat aaacataaaa aatgtagagg tcgagtttag
     5881 atgcaagttc aaggagcgaa aggtggatgg gtaggttata tagggatata gcacagagat
     5941 atatagcaaa gagatacttt tgagcaatgt ttgtggaagc ggtattcgca atattttagt
     6001 agctcgttac agtccggtgc gtttttggtt ttttgaaagt gcgtcttcag agcgcttttg
     6061 gttttcaaaa gcgctctgaa gttcctatac tttctagaga ataggaactt cggaatagga
     6121 acttcaaagc gtttccgaaa acgagcgctt ccgaaaatgc aacgcgagct gcgcacatac
     6181 agctcactgt tcacgtcgca cctatatctg cgtgttgcct gtatatatat atacatgaga
     6241 agaacggcat agtgcgtgtt tatgcttaaa tgcgtactta tatgcgtcta tttatgtagg
     6301 atgaaaggta gtctagtacc tcctgtgata ttatcccatt ccatgcgggg tatcgtatgc
     6361 ttccttcagc actacccttt agctgttcta tatgctgcca ctcctcaatt ggattagtct
     6421 catccttcaa tgctatcatt tcctttgata ttggatcata ttaagaaacc attattatca
     6481 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtc
//
