LOCUS       .                       4470 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             2868..3677
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /note="confers resistance to kanamycin in bacteria or G418
                     (Geneticin®) in eukaryotes"
                     /note="color: #ccffcc"
                     /product="aminoglycoside phosphotransferase"
                     /transl_table=1
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"
                     /label="KanR"
     CDS             complement(1606..2265)
                     /codon_start=1
                     /gene="cat"
                     /note="confers resistance to chloramphenicol"
                     /note="color: #ccffcc"
                     /product="chloramphenicol acetyltransferase"
                     /transl_table=1
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA*"
                     /label="CmR"
     rep_origin      3820..4408
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /label="ori"
     CDS             complement(959..1264)
                     /codon_start=1
                     /gene="ccdB"
                     /note="Plasmids containing the ccdB gene cannot be
                     propagated in standard E. coli strains."
                     /note="color: #993366"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /transl_table=1
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI*"
                     /label="ccdB"
     protein_bind    332..563
                     /bound_moiety="BP Clonase™"
                     /gene="mutant version of attP"
                     /note="recombination site for the Gateway® BP reaction
                     (pDONR™201 version)"
                     /note="color: #31849b"
                     /label="attP1"
     protein_bind    complement(2513..2744)
                     /bound_moiety="BP Clonase™"
                     /gene="mutant version of attP"
                     /note="recombination site for the Gateway® BP reaction
                     (pDONR™201 version)"
                     /note="color: #31849b"
                     /label="attP2"
     promoter        complement(2266..2368)
                     /note="promoter of the E. coli cat gene"
                     /note="color: #ffffff"
                     /label="cat promoter"
     terminator      192..278
                     /gene="Escherichia coli rrnB"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
                     /note="color: #ffffff"
                     /label="rrnB T1 terminator"
     terminator      73..100
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
                     /note="color: #ffffff"
                     /label="rrnB T2 terminator"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagcc aggaagagtt
       61 tgtagaaacg caaaaaggcc atccgtcagg atggccttct gcttagtttg atgcctggca
      121 gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc
      181 gctcccggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga
      241 aaggcccagt cttccgactg agcctttcgt tttatttgat gcctggcagt tccctactct
      301 cgcgttaacg ctagcatgga tctcgggccc caaataatga ttttattttg actgatagtg
      361 acctgttcgt tgcaacaaat tgatgagcaa tgctttttta taatgccaag tttgtacaaa
      421 aaagcagaac gagaaacgta aaatgatata aatatcaata tattaaatta gattttgcat
      481 aaaaaacaga ctacataata ctgtaaaaca caacatatcc agtcactatg aatcaactac
      541 ttagatggta ttagtgacct gtagtcgacc gacagccttc caaatgttct tcgggtgatg
      601 ctgccaactt agtcgaccga cagccttcca aatgttcttc tcaaacggaa tcgtcgtatc
      661 cagcctactc gctattgtcc tcaatgccgt attaaatcat aaaaagaaat aagaaaaaga
      721 ggtgcgagcc tcttttttgt gtgacaaaat aaaaacatct acctattcat atacgctagt
      781 gtcatagtcc tgaaaatcat ctgcatcaag aacaatttca caactcttat acttttctct
      841 tacaagtcgt tcggcttcat ctggattttc agcctctata cttactaaac gtgataaagt
      901 ttctgtaatt tctactgtat cgacctgcag actggctgtg tataagggag cctgacattt
      961 atattcccca gaacatcagg ttaatggcgt ttttgatgtc attttcgcgg tggctgagat
     1021 cagccacttc ttccccgata acggagaccg gcacactggc catatcggtg gtcatcatgc
     1081 gccagctttc atccccgata tgcaccaccg ggtaaagttc acgggagact ttatctgaca
     1141 gcagacgtgc actggccagg gggatcacca tccgtcgccc gggcgtgtca ataatatcac
     1201 tctgtacatc cacaaacaga cgataacggc tctctctttt ataggtgtaa accttaaact
     1261 gcatttcacc agtccctgtt ctcgtcagca aaagagccgt tcatttcaat aaaccgggcg
     1321 acctcagcca tcccttcctg attttccgct ttccagcgtt cggcacgcag acgacgggct
     1381 tcattctgca tggttgtgct taccagaccg gagatattga catcatatat gccttgagca
     1441 actgatagct gtcgctgtca actgtcactg taatacgctg cttcatagca cacctctttt
     1501 tgacatactt cgggtataca tatcagtata tattcttata ccgcaaaaat cagcgcgcaa
     1561 atacgcatac tgttatctgg cttttagtaa gccggatcca cgcgattacg ccccgccctg
     1621 ccactcatcg cagtactgtt gtaattcatt aagcattctg ccgacatgga agccatcaca
     1681 gacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt gcgtataata
     1741 tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa
     1801 actggtgaaa ctcacccagg gattggctga gacgaaaaac atattctcaa taaacccttt
     1861 agggaaatag gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa
     1921 ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa aacgtttcag tttgctcatg
     1981 gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc
     2041 catacggaat tccggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata
     2101 aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctgaacggt
     2161 ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca
     2221 ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc
     2281 tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg
     2341 aaagttggaa cctcttacgt gccgatcaac gtctcatttt cgccaaaagt tggcccaggg
     2401 cttcccggta tcaacaggga caccaggatt tatttattct gcgaagtgat cttccgtcac
     2461 aggtatttat tcggcgcaaa gtgcgtcggg tgatgctgcc aacttagtcg actacaggtc
     2521 actaatacca tctaagtagt tgattcatag tgactggata tgttgtgttt tacagtatta
     2581 tgtagtctgt tttttatgca aaatctaatt taatatattg atatttatat cattttacgt
     2641 ttctcgttca gctttcttgt acaaagtggg cattataaga aagcattgct tatcaatttg
     2701 ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt atttgccatc cagctgcagc
     2761 tctggcccgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat
     2821 gaacaataaa actgtctgct tacataaaca gtaatacaag gggtgttatg agccatattc
     2881 aacgggaaac gtcgaggccg cgattaaatt ccaacatgga tgctgattta tatgggtata
     2941 aatgggctcg cgataatgtc gggcaatcag gtgcgacaat ctatcgcttg tatgggaagc
     3001 ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag cgttgccaat gatgttacag
     3061 atgagatggt cagactaaac tggctgacgg aatttatgcc tcttccgacc atcaagcatt
     3121 ttatccgtac tcctgatgat gcatggttac tcaccactgc gatccccgga aaaacagcat
     3181 tccaggtatt agaagaatat cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt
     3241 tcctgcgccg gttgcattcg attcctgttt gtaattgtcc ttttaacagc gatcgcgtat
     3301 ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg agtgattttg
     3361 atgacgagcg taatggctgg cctgttgaac aagtctggaa agaaatgcat aaacttttgc
     3421 cattctcacc ggattcagtc gtcactcatg gtgatttctc acttgataac cttatttttg
     3481 acgaggggaa attaataggt tgtattgatg ttggacgagt cggaatcgca gaccgatacc
     3541 aggatcttgc catcctatgg aactgcctcg gtgagttttc tccttcatta cagaaacggc
     3601 tttttcaaaa atatggtatt gataatcctg atatgaataa attgcagttt catttgatgc
     3661 tcgatgagtt tttctaatca gaattggtta attggttgta acactggcag agcattacgc
     3721 tgacttgacg ggacggcgca agctcatgac caaaatccct taacgtgagt tttcgttcca
     3781 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     3841 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     3901 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     3961 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     4021 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     4081 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     4141 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     4201 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     4261 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     4321 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     4381 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     4441 ggccttttgc tggccttttg ctcacatgtt
//
