LOCUS       .                       7481 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             5417..6277
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ccffcc"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     CDS             4100..4894
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /note="color: #ccffcc"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     CDS             904..1563
                     /codon_start=1
                     /gene="cat"
                     /note="confers resistance to chloramphenicol"
                     /note="color: #ccffcc"
                     /product="chloramphenicol acetyltransferase"
                     /transl_table=1
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA*"
                     /label="CmR"
     rep_origin      6448..7036
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /label="ori"
     rep_origin      3118..3573
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
                     /label="f1 ori"
     promoter        3692..4049
                     /note="SV40 enhancer and early promoter"
                     /note="color: #ffffff"
                     /label="SV40 promoter"
     CDS             1905..2210
                     /codon_start=1
                     /gene="ccdB"
                     /note="Plasmids containing the ccdB gene cannot be
                     propagated in standard E. coli strains."
                     /note="color: #993366"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /transl_table=1
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI*"
                     /label="ccdB"
     enhancer        15..318
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
                     /label="CMV enhancer"
     promoter        319..522
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /note="color: #ffffff"
                     /label="CMV promoter"
     rep_origin      3900..4035
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
                     /label="SV40 ori"
     polyA_signal    2858..2992
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
                     /label="SV40 poly(A) signal"
     protein_bind    671..795
                     /bound_moiety="LR Clonase™"
                     /gene="mutant version of attR"
                     /note="recombination site for the Gateway® LR reaction"
                     /note="color: #31849b"
                     /label="attR1"
     protein_bind    complement(2251..2375)
                     /bound_moiety="LR Clonase™"
                     /gene="mutant version of attR"
                     /note="recombination site for the Gateway® LR reaction"
                     /note="color: #31849b"
                     /label="attR2"
     promoter        5312..5416
                     /gene="bla"
                     /label="AmpR promoter"
                     /note="color: #ffffff"
     polyA_signal    4958..5006
                     /note="synthetic polyadenylation signal"
                     /note="color: #a6acb3"
                     /label="poly(A) signal"
     promoter        820..850
                     /note="E. coli lac promoter with an 'up' mutation"
                     /note="color: #ffffff"
                     /label="lac UV5 promoter"
     promoter        complement(2408..2426)
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff"
                     /label="T7 promoter"
     CDS             644..661
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /transl_table=1
                     /translation="HHHHHH"
                     /label="6xHis"
                     /note="color: #cc99b2"
     primer_bind     complement(2437..2453)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /note="color: #a020f0"
                     /label="M13 fwd"
     CDS             632..634
                     /codon_start=1
                     /product="start codon"
                     /transl_table=1
                     /translation="M"
                     /label="ATG"
                     /note="color: #993366"
ORIGIN
        1 cattagatgc atgtcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
       61 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
      121 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
      181 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
      241 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
      301 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
      361 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
      421 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
      481 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctcgtttagt gaaccgtcag
      541 atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc
      601 agcctccgga ctctagccta ggccgcggac catggcgtac taccatcacc atcaccatca
      661 ctctagatca acaagtttgt acaaaaaagc tgaacgagaa acgtaaaatg atataaatat
      721 caatatatta aattagattt tgcataaaaa acagactaca taatactgta aaacacaaca
      781 tatccagtca ctatggcggc cgcattaggc accccaggct ttacacttta tgcttccggc
      841 tcgtataatg tgtggatttt gagttaggat ccggcgagat tttcaggagc taaggaagct
      901 aaaatggaga aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa
      961 gaacattttg aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg
     1021 gatattacgg cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt
     1081 attcacattc ttgcccgcct gatgaatgct catccggaat tccgtatggc aatgaaagac
     1141 ggtgagctgg tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact
     1201 gaaacgtttt catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata
     1261 tattcgcaag atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt
     1321 gagaatatgt ttttcgtctc agccaatccc tgggtgagtt tcaccagttt tgatttaaac
     1381 gtggccaata tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa
     1441 ggcgacaagg tgctgatgcc gctggcgatt caggttcatc atgccgtctg tgatggcttc
     1501 catgtcggca gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg
     1561 taaagatctg gatccggctt actaaaagcc agataacagt atgcgtattt gcgcgctgat
     1621 ttttgcggta taagaatata tactgatatg tatacccgaa gtatgtcaaa aagaggtgtg
     1681 ctatgaagca gcgtattaca gtgacagttg acagcgacag ctatcagttg ctcaaggcat
     1741 atatgatgtc aatatctccg gtctggtaag cacaaccatg cagaatgaag cccgtcgtct
     1801 gcgtgccgaa cgctggaaag cggaaaatca ggaagggatg gctgaggtcg cccggtttat
     1861 tgaaatgaac ggctcttttg ctgacgagaa cagggactgg tgaaatgcag tttaaggttt
     1921 acacctataa aagagagagc cgttatcgtc tgtttgtgga tgtacagagt gatattattg
     1981 acacgcccgg gcgacggatg gtgatccccc tggccagtgc acgtctgctg tcagataaag
     2041 tctcccgtga actttacccg gtggtgcata tcggggatga aagctggcgc atgatgacca
     2101 ccgatatggc cagtgtgccg gtctccgtta tcggggaaga agtggctgat ctcagccacc
     2161 gcgaaaatga catcaaaaac gccattaacc tgatgttctg gggaatataa atgtcaggct
     2221 cccttataca cagccagtct gcaggtcgac catagtgact ggatatgttg tgttttacag
     2281 tattatgtag tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt
     2341 tacgtttctc gttcagcttt cttgtacaaa gtggttgatc gcgtgcatgc gacgtcatag
     2401 ctctctccct atagtgagtc gtattataag ctaggcactg gccgtcgttt tacaacgtcg
     2461 tgactgggaa aactgctagc ttgggatctt tgtgaaggaa ccttacttct gtggtgtgac
     2521 ataattggac aaactaccta cagagattta aagctctaag gtaaatataa aatttttaag
     2581 tgtataatgt gttaaactag ctgcatatgc ttgctgcttg agagttttgc ttactgagta
     2641 tgatttatga aaatattata cacaggagct agtgattcta attgtttgtg tattttagat
     2701 tcacagtccc aaggctcatt tcaggcccct cagtcctcac agtctgttca tgatcataat
     2761 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
     2821 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
     2881 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     2941 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcgatcctgc
     3001 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattggctgg cgtaatagcg
     3061 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg
     3121 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     3181 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     3241 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     3301 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     3361 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     3421 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     3481 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa atatttaacg
     3541 cgaattttaa caaaatatta acgtttacaa tttcgcctga tgcggtattt tctccttacg
     3601 catctgtgcg gtatttcaca ccgcatacgc ggatctgcgc agcaccatgg cctgaaataa
     3661 cctctgaaag aggaacttgg ttaggtacct tctgaggcgg aaagaaccag ctgtggaatg
     3721 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     3781 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     3841 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     3901 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     3961 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     4021 gcttttttgg aggcctaggc ttttgcaaaa agcttgattc ttctgacaca acagtctcga
     4081 acttaaggct agagccacca tgattgaaca agatggattg cacgcaggtt ctccggccgc
     4141 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc
     4201 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc
     4261 cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg
     4321 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
     4381 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc
     4441 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
     4501 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
     4561 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct
     4621 caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc
     4681 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
     4741 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
     4801 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
     4861 cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc
     4921 gaccaagcga cgcccaacct gccatcacga tggccgcaat aaaatatctt tattttcatt
     4981 acatctgtgt gttggttttt tgtgtgaatc gatagcgata aggatccgcg tatggtgcac
     5041 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc
     5101 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     5161 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg
     5221 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
     5281 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     5341 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     5401 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     5461 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     5521 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     5581 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     5641 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     5701 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     5761 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     5821 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     5881 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     5941 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     6001 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     6061 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     6121 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     6181 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     6241 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     6301 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     6361 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     6421 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     6481 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     6541 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     6601 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     6661 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     6721 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     6781 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagca
     6841 ttgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     6901 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     6961 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     7021 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     7081 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     7141 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     7201 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     7261 tcattaatgc agagcttgca attcgcgcgt ttttcaatat tattgaagca tttatcaggg
     7321 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     7381 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
     7441 attaacctat aaaaataggc gtagtacgag gccctttcac t
//
