LOCUS       .                       6961 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             3181..4041
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ccffcc"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     CDS             304..963
                     /codon_start=1
                     /gene="cat"
                     /note="confers resistance to chloramphenicol"
                     /note="color: #ccffcc"
                     /product="chloramphenicol acetyltransferase"
                     /transl_table=1
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA*"
                     /label="CmR"
     CDS             1780..2433
                     /codon_start=1
                     /gene=""
                     /product="glutathione S-transferase from Schistosoma
                     japonicum"
                     /transl_table=1
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
                     /label="GST"
                     /note="color: #cc99b2"
     rep_origin      4212..4800
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /label="ori"
     CDS             1305..1610
                     /codon_start=1
                     /gene="ccdB"
                     /note="Plasmids containing the ccdB gene cannot be
                     propagated in standard E. coli strains."
                     /note="color: #993366"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /transl_table=1
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI*"
                     /label="ccdB"
     CDS             complement(5230..5421)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /transl_table=1
                     /translation="VTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL*"
                     /label="rop"
                     /note="color: #993366"
     protein_bind    71..195
                     /bound_moiety="LR Clonase™"
                     /gene="mutant version of attR"
                     /note="recombination site for the Gateway® LR reaction"
                     /note="color: #31849b"
                     /label="attR1"
     protein_bind    complement(1651..1775)
                     /bound_moiety="LR Clonase™"
                     /gene="mutant version of attR"
                     /note="recombination site for the Gateway® LR reaction"
                     /note="color: #31849b"
                     /label="attR2"
     promoter        3076..3180
                     /gene="bla"
                     /label="AmpR promoter"
                     /note="color: #ffffff"
     terminator      2522..2569
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
                     /note="color: #ffffff"
                     /label="T7 terminator"
     promoter        220..250
                     /note="E. coli lac promoter with an 'up' mutation"
                     /note="color: #ffffff"
                     /label="lac UV5 promoter"
     promoter        25..43
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff"
                     /label="T7 promoter"
ORIGIN
        1 atcgagatct cgatcccgcg aaattaatac gactcactat agggagacca caacggtttc
       61 cctctagatc acaagtttgt acaaaaaagc tgaacgagaa acgtaaaatg atataaatat
      121 caatatatta aattagattt tgcataaaaa acagactaca taatactgta aaacacaaca
      181 tatccagtca ctatggcggc cgcattaggc accccaggct ttacacttta tgcttccggc
      241 tcgtataatg tgtggatttt gagttaggat ccggcgagat tttcaggagc taaggaagct
      301 aaaatggaga aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa
      361 gaacattttg aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg
      421 gatattacgg cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt
      481 attcacattc ttgcccgcct gatgaatgct catccggaat tccgtatggc aatgaaagac
      541 ggtgagctgg tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact
      601 gaaacgtttt catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata
      661 tattcgcaag atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt
      721 gagaatatgt ttttcgtctc agccaatccc tgggtgagtt tcaccagttt tgatttaaac
      781 gtggccaata tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa
      841 ggcgacaagg tgctgatgcc gctggcgatt caggttcatc atgccgtctg tgatggcttc
      901 catgtcggca gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg
      961 taaacgcgtg gatccggctt actaaaagcc agataacagt atgcgtattt gcgcgctgat
     1021 ttttgcggta taagaatata tactgatatg tatacccgaa gtatgtcaaa aagaggtgtg
     1081 ctatgaagca gcgtattaca gtgacagttg acagcgacag ctatcagttg ctcaaggcat
     1141 atatgatgtc aatatctccg gtctggtaag cacaaccatg cagaatgaag cccgtcgtct
     1201 gcgtgccgaa cgctggaaag cggaaaatca ggaagggatg gctgaggtcg cccggtttat
     1261 tgaaatgaac ggctcttttg ctgacgagaa cagggactgg tgaaatgcag tttaaggttt
     1321 acacctataa aagagagagc cgttatcgtc tgtttgtgga tgtacagagt gatattattg
     1381 acacgcccgg gcgacggatg gtgatccccc tggccagtgc acgtctgctg tcagataaag
     1441 tctcccgtga actttacccg gtggtgcata tcggggatga aagctggcgc atgatgacca
     1501 ccgatatggc cagtgtgccg gtctccgtta tcggggaaga agtggctgat ctcagccacc
     1561 gcgaaaatga catcaaaaac gccattaacc tgatgttctg gggaatataa atgtcaggct
     1621 cccttataca cagccagtct gcaggtcgac catagtgact ggatatgttg tgttttacag
     1681 tattatgtag tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt
     1741 tacgtttctc gttcagcttt cttgtacaaa gtggtgatta tgtcccctat actaggttat
     1801 tggaaaatta agggccttgt gcaacccact cgacttcttt tggaatatct tgaagaaaaa
     1861 tatgaagagc atttgtatga gcgcgatgaa ggtgataaat ggcgaaacaa aaagtttgaa
     1921 ttgggtttgg agtttcccaa tcttccttat tatattgatg gtgatgttaa attaacacag
     1981 tctatggcca tcatacgtta tatagctgac aagcacaaca tgttgggtgg ttgtccaaaa
     2041 gagcgtgcag agatttcaat gcttgaagga gcggttttgg atattagata cggtgtttcg
     2101 agaattgcat atagtaaaga ctttgaaact ctcaaagttg attttcttag caagctacct
     2161 gaaatgctga aaatgttcga agatcgttta tgtcataaaa catatttaaa tggtgatcat
     2221 gtaacccatc ctgacttcat gttgtatgac gctcttgatg ttgttttata catggaccca
     2281 atgtgcctgg atgcgttccc aaaattagtt tgttttaaaa aacgtattga agctatccca
     2341 caaattgata agtacttgaa atccagcaag tatatagcat ggcctttgca gggctggcaa
     2401 gccacgtttg gtggtggcga ccatcctcca aaatcggatc tggttccgcg tccatgggga
     2461 tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata
     2521 actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg
     2581 aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg
     2641 ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga
     2701 gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac
     2761 tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca
     2821 agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag
     2881 atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa
     2941 gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata
     3001 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     3061 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     3121 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     3181 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     3241 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     3301 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     3361 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     3421 cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     3481 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     3541 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     3601 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     3661 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     3721 cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     3781 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     3841 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     3901 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     3961 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     4021 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     4081 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     4141 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     4201 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     4261 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     4321 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     4381 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     4441 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     4501 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     4561 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     4621 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     4681 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     4741 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     4801 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     4861 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     4921 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     4981 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat
     5041 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct
     5101 atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc
     5161 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
     5221 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag
     5281 ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc
     5341 gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc
     5401 ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt
     5461 aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc
     5521 ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag
     5581 aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg
     5641 tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg
     5701 cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc
     5761 agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta
     5821 accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg
     5881 cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc
     5941 ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa
     6001 ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt
     6061 caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat
     6121 agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc
     6181 gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct
     6241 tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc
     6301 atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc
     6361 gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc
     6421 ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag
     6481 attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg
     6541 ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca
     6601 gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg
     6661 aaggctctca agggcatcgg tcgatcgacg ctctccctta tgcgactcct gcattaggaa
     6721 gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa
     6781 ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca
     6841 agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata
     6901 ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag
     6961 g
//
