LOCUS       .                       7012 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             3232..4092
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ccffcc"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     CDS             1025..1705
                     /codon_start=1
                     /note="confers resistance to chloramphenicol"
                     /note="color: #ccffcc"
                     /product="chloramphenicol acetyltransferase"
                     /transl_table=1
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY*"
                     /label="CmR"
     CDS             105..758
                     /codon_start=1
                     /gene=""
                     /product="glutathione S-transferase from Schistosoma
                     japonicum"
                     /transl_table=1
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
                     /label="GST"
                     /note="color: #cc99b2"
     rep_origin      4263..4851
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /label="ori"
     CDS             2025..2330
                     /codon_start=1
                     /gene="ccdB"
                     /note="Plasmids containing the ccdB gene cannot be
                     propagated in standard E. coli strains."
                     /note="color: #993366"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /transl_table=1
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI*"
                     /label="ccdB"
     CDS             complement(5281..5472)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /transl_table=1
                     /translation="VTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL*"
                     /label="rop"
                     /note="color: #993366"
     protein_bind    792..916
                     /bound_moiety="LR Clonase™"
                     /gene="mutant version of attR"
                     /note="recombination site for the Gateway® LR reaction"
                     /note="color: #31849b"
                     /label="attR1"
     protein_bind    complement(2371..2495)
                     /bound_moiety="LR Clonase™"
                     /gene="mutant version of attR"
                     /note="recombination site for the Gateway® LR reaction"
                     /note="color: #31849b"
                     /label="attR2"
     promoter        3127..3231
                     /gene="bla"
                     /label="AmpR promoter"
                     /note="color: #ffffff"
     terminator      2573..2620
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
                     /note="color: #ffffff"
                     /label="T7 terminator"
     promoter        941..971
                     /note="E. coli lac promoter with an 'up' mutation"
                     /note="color: #ffffff"
                     /label="lac UV5 promoter"
     promoter        25..43
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff"
                     /label="T7 promoter"
     RBS             90..96
                     /note="ribosome binding site"
                     /note="color: #a6acb3"
                     /label="RBS"
ORIGIN
        1 atcgagatct cgatcccgcg aaattaatac gactcactat agggagacca caacggtttc
       61 cctctagaaa taattttgtt taactttaag aaggagatat acatatgtcc cctatactag
      121 gttattggaa aattaagggc cttgtgcaac ccactcgact tcttttggaa tatcttgaag
      181 aaaaatatga agagcatttg tatgagcgcg atgaaggtga taaatggcga aacaaaaagt
      241 ttgaattggg tttggagttt cccaatcttc cttattatat tgatggtgat gttaaattaa
      301 cacagtctat ggccatcata cgttatatag ctgacaagca caacatgttg ggtggttgtc
      361 caaaagagcg tgcagagatt tcaatgcttg aaggagcggt tttggatatt agatacggtg
      421 tttcgagaat tgcatatagt aaagactttg aaactctcaa agttgatttt cttagcaagc
      481 tacctgaaat gctgaaaatg ttcgaagatc gtttatgtca taaaacatat ttaaatggtg
      541 atcatgtaac ccatcctgac ttcatgttgt atgacgctct tgatgttgtt ttatacatgg
      601 acccaatgtg cctggatgcg ttcccaaaat tagtttgttt taaaaaacgt attgaagcta
      661 tcccacaaat tgataagtac ttgaaatcca gcaagtatat agcatggcct ttgcagggct
      721 ggcaagccac gtttggtggt ggcgaccatc ctccaaaatc ggatctggtt ccgcgtccat
      781 ggtcgaatca aacaagtttg tacaaaaaag ctgaacgaga aacgtaaaat gatataaata
      841 tcaatatatt aaattagatt ttgcataaaa aacagactac ataatactgt aaaacacaac
      901 atatccagtc actatggcgg ccgcattagg caccccaggc tttacacttt atgcttccgg
      961 ctcgtataat gtgtggattt tgagttagga tccgtcgaga ttttcaggag ctaaggaagc
     1021 taaaatggag aaaaaaatca ctggatatac caccgttgat atatcccaat ggcatcgtaa
     1081 agaacatttt gaggcatttc agtcagttgc tcaatgtacc tataaccaga ccgttcagct
     1141 ggatattacg gcctttttaa agaccgtaaa gaaaaataag cacaagtttt atccggcctt
     1201 tattcacatt cttgcccgcc tgatgaatgc tcatccggaa ttccgtatgg caatgaaaga
     1261 cggtgagctg gtgatatggg atagtgttca cccttgttac accgttttcc atgagcaaac
     1321 tgaaacgttt tcatcgctct ggagtgaata ccacgacgat ttccggcagt ttctacacat
     1381 atattcgcaa gatgtggcgt gttacggtga aaacctggcc tatttcccta aagggtttat
     1441 tgagaatatg tttttcgtct cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa
     1501 cgtggccaat atggacaact tcttcgcccc cgttttcacc atgggcaaat attatacgca
     1561 aggcgacaag gtgctgatgc cgctggcgat tcaggttcat catgccgttt gtgatggctt
     1621 ccatgtcggc agaatgctta atgaattaca acagtactgc gatgagtggc aggcggggcg
     1681 taatctagag gatccggctt actaaaagcc agataacagt atgcgtattt gcgcgctgat
     1741 ttttgcggta taagaatata tactgatatg tatacccgaa gtatgtcaaa aagaggtatg
     1801 ctatgaagca gcgtattaca gtgacagttg acagcgacag ctatcagttg ctcaaggcat
     1861 atatgatgtc aatatctccg gtctggtaag cacaaccatg cagaatgaag cccgtcgtct
     1921 gcgtgccgaa cgctggaaag cggaaaatca ggaagggatg gctgaggtcg cccggtttat
     1981 tgaaatgaac ggctcttttg ctgacgagaa caggggctgg tgaaatgcag tttaaggttt
     2041 acacctataa aagagagagc cgttatcgtc tgtttgtgga tgtacagagt gatattattg
     2101 acacgcccgg gcgacggatg gtgatccccc tggccagtgc acgtctgctg tcagataaag
     2161 tcccccgtga actttacccg gtggtgcata tcggggatga aagctggcgc atgatgacca
     2221 ccgatatggc cagtgtgccg gtctccgtta tcggggaaga agtggctgat ctcagccacc
     2281 gcgaaaatga catcaaaaac gccattaacc tgatgttctg gggaatataa atgtcaggct
     2341 cccttataca cagccagtct gcaggtcgac catagtgact ggatatgttg tgttttacag
     2401 tattatgtag tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt
     2461 tacgtttctc gttcagcttt cttgtacaaa gtggtttgat tcgacccggg atccggctgc
     2521 taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata
     2581 accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc
     2641 cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta
     2701 gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg
     2761 cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc
     2821 tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc
     2881 ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac
     2941 acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga
     3001 tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt
     3061 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     3121 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     3181 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     3241 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     3301 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     3361 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     3421 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac
     3481 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     3541 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     3601 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     3661 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     3721 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca
     3781 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     3841 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     3901 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     3961 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     4021 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     4081 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     4141 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     4201 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     4261 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     4321 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     4381 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     4441 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     4501 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     4561 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     4621 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     4681 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     4741 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     4801 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     4861 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     4921 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     4981 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg
     5041 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc
     5101 tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg
     5161 tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
     5221 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
     5281 tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc
     5341 gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt
     5401 ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc
     5461 ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc
     5521 gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact
     5581 ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac
     5641 tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca
     5701 gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag
     5761 actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt
     5821 gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag
     5881 gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg
     5941 ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat
     6001 ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc
     6061 tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag
     6121 gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg
     6181 cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg
     6241 atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt
     6301 ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg
     6361 ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc
     6421 agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg
     6481 aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat
     6541 accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg
     6601 acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt
     6661 gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc
     6721 aagggcatcg gtcgatcgac gctctccctt atgcgactcc tgcattagga agcagcccag
     6781 tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc
     6841 gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat
     6901 gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc
     6961 aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga gg
//
