LOCUS       .                       4555 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     protein_bind    complement(2065..2188)
                     /bound_moiety="LR Clonase™"
                     /gene="mutant version of attR"
                     /note="recombination site for the Gateway® LR reaction"
                     /note="color: #31849b"
                     /label="attR3"
     CDS             2791..3651
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ccffcc"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGSDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     CDS             complement(1163..1816)
                     /codon_start=1
                     /gene="cat"
                     /note="confers resistance to chloramphenicol"
                     /note="color: #ccffcc"
                     /product="chloramphenicol acetyltransferase"
                     /transl_table=1
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG"
                     /label="CmR"
     rep_origin      3822..4410
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /label="ori"
     CDS             complement(508..813)
                     /codon_start=1
                     /gene="ccdB"
                     /note="Plasmids containing the ccdB gene cannot be
                     propagated in standard E. coli strains."
                     /note="color: #993366"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /transl_table=1
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI*"
                     /label="ccdB"
     protein_bind    36..160
                     /bound_moiety="LR Clonase™"
                     /gene="mutant version of attR"
                     /note="recombination site for the Gateway® LR reaction"
                     /note="color: #31849b"
                     /label="attR4"
     promoter        2686..2790
                     /gene="bla"
                     /label="AmpR promoter"
                     /note="color: #ffffff"
     promoter        complement(1817..1919)
                     /note="promoter of the E. coli cat gene"
                     /note="color: #ffffff"
                     /label="cat promoter"
     primer_bind     1..17
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /note="color: #a020f0"
                     /label="M13 rev"
     primer_bind     complement(2196..2212)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /note="color: #a020f0"
                     /label="M13 fwd"
ORIGIN
        1 caggaaacag ctatgaccat gattacgcca agctatcaac tttgtataga aaagttgaac
       61 gagaaacgta aaatgatata aatatcaata tattaaatta gattttgcat aaaaaacaga
      121 ctacataata ctgtaaaaca caacatatcc agtcactatg gtcgaccgac agccttccaa
      181 atgttcttct caaacggaat cgtcgtatcc agcctactcg ctattgtcct caatgccgta
      241 ttaaatcata aaaagaaata agaaaaagag gtgcgagcct cttttttgtg tgacaaaata
      301 aaaacatcta cctattcata tacgctagtg tcatagtcct gaaaatcatc tgcatcaaga
      361 acaatttcac aactcttata cttttctctt acaagtcgtt cggcttcatc tggattttca
      421 gcctctatac ttactaaacg tgataaagtt tctgtaattt ctactgtatc gacctgcaga
      481 ctggctgtgt ataagggagc ctgacattta tattccccag aacatcaggt taatggcgtt
      541 tttgatgtca ttttcgcggt ggctgagatc agccacttct tccccgataa cggagaccgg
      601 cacactggcc atatcggtgg tcatcatgcg ccagctttca tccccgatat gcaccaccgg
      661 gtaaagttca cgggagactt tatctgacag cagacgtgca ctggccaggg ggatcaccat
      721 ccgtcgcccg ggcgtgtcaa taatatcact ctgtacatcc acaaacagac gataacggct
      781 ctctctttta taggtgtaaa ccttaaactg catttcacca gcccctgttc tcgtcagcaa
      841 aagagccgtt catttcaata aaccgggcga cctcagccat cccttcctga ttttccgctt
      901 tccagcgttc ggcacgcaga cgacgggctt cattctgcat ggttgtgctt accagaccgg
      961 agatattgac atcatatatg ccttgagcaa ctgatagctg tcgctgtcaa ctgtcactgt
     1021 aatacgctgc ttcatagcat acctcttttt gacatacttc gggtatacat atcagtatat
     1081 attcttatac cgcaaaaatc agcgcgcaaa tacgcatact gttatctggc ttttagtaag
     1141 ccggatccac gcggcgttta cgccccccct gccactcatc gcagtactgt tgtaattcat
     1201 taagcattct gccgacatgg aagccatcac aaacggcatg atgaacctga atcgccagcg
     1261 gcatcagcac cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga
     1321 agttgtccat attggccacg tttaaatcaa aactggtgaa actcacccag ggattggctg
     1381 agacgaaaaa catattctca ataaaccctt tagggaaata ggccaggttt tcaccgtaac
     1441 acgccacatc ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg tattcactcc
     1501 agagcgatga aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg tgaacactat
     1561 cccatatcac cagctcaccg tctttcattg ccatacggaa ttccggatga gcattcatca
     1621 ggcgggcaag aatgtgaata aaggccggat aaaacttgtg cttatttttc tttacggtct
     1681 ttaaaaaggc cgtaatatcc agctgaacgg tctggttata ggtacattga gcaactgact
     1741 gaaatgcctc aaaatgttct ttacgatgcc attgggatat atcaacggtg gtatatccag
     1801 tgattttttt ctccatttta gcttccttag ctcctgaaaa tctcgataac tcaaaaaata
     1861 cgcccggtag tgatcttatt tcattatggt gaaagttgga acctcttacg tgccgatcaa
     1921 cgtctcattt tcgccaaaag ttggcccagg gcttcccggt atcaacaggg acaccaggat
     1981 ttatttattc tgcgaagtga tcttccgtca caggtattta ttcggcgcaa agtgcgtcgg
     2041 gtgatgctgc caacttacgg ccgccatagt gactggatat gttgtgtttt acagtattat
     2101 gtagtctgtt ttttatgcaa aatctaattt aatatattga tatttatatc attttacgtt
     2161 tctcgttcaa ctttattata catagttgat aattcactgg ccgtcgtttt acaacgtcgt
     2221 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
     2281 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     2341 aatggcgaat ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
     2401 cgcatatggt gcactctcag tgcaatctgc tctgatgccg catagttaag ccagccccga
     2461 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     2521 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     2581 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     2641 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     2701 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     2761 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     2821 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     2881 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     2941 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     3001 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     3061 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     3121 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     3181 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     3241 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     3301 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     3361 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     3421 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     3481 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggtcagat
     3541 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     3601 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     3661 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     3721 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     3781 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     3841 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     3901 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     3961 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     4021 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     4081 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     4141 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     4201 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     4261 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     4321 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     4381 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     4441 ctggtctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     4501 gataaccgta ttaccgcctt tgagtgagct gataccagcg gataacaatt tcaca
//
