LOCUS . 6219 bp DNA circular UNK 01-JAN-1980 DEFINITION . ACCESSION VERSION KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers CDS complement(4459..5253) /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /note="color: #ccffcc" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" CDS complement(3159..3857) /codon_start=1 /note="mammalian codon-optimized" /note="color: #05fd14" /product="improved green fluorescent protein from Pontellina plumata (Evdokimov et al., 2006)" /transl_table=1 /translation="MESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKM KSTKGALTFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVL HVSFSYRYEAGRVIGDFKVMGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNDLDGSFTR TFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFAFRRVEEDHSNTELGIVEYQHAF KTPDADAGEE*" /label="TurboGFP" polyA_signal 1202..1824 /note="human growth hormone polyadenylation signal" /note="color: #a6acb3" /label="hGH poly(A) signal" rep_origin complement(1973..2561) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /note="color: #ffff00" /label="ori" misc_feature 3869..4421 /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" /note="color: #a6acb3" /label="IRES" rep_origin 5778..6206 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /note="color: #ffff00" /label="f1 ori" enhancer 343..722 /note="human cytomegalovirus immediate early enhancer" /note="color: #ffffff" /label="CMV enhancer" promoter complement(5288..5645) /note="SV40 enhancer and early promoter" /note="color: #ffffff" /label="SV40 promoter" promoter 723..926 /note="human cytomegalovirus (CMV) immediate early promoter" /note="color: #ffffff" /label="CMV promoter" rep_origin 5302..5437 /note="SV40 origin of replication" /note="color: #ffff00" /label="SV40 ori" promoter complement(5647..5751) /gene="bla" /label="AmpR promoter" /note="color: #ffffff" polyA_signal 2890..2937 /note="herpesvirus thymidine kinase polyadenylation signal" /note="color: #a6acb3" /label="HSV TK poly(A) signal" CDS 1084..1113 /codon_start=1 /product="Myc (human c-Myc oncogene) epitope tag" /transl_table=1 /translation="EQKLISEEDL" /label="Myc" /note="color: #cc99b2" CDS 1132..1155 /codon_start=1 /product="FLAG epitope tag, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDDDDK" /label="FLAG" /note="color: #cc99b2" promoter 952..970 /note="promoter for bacteriophage T7 RNA polymerase" /note="color: #ffffff" /label="T7 promoter" ORIGIN 1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag 61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa 121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca 181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt 241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata 301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta 361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg 421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga 481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat 541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa 601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca 661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca 721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat 781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg 841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac 901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac 961 tcactatagg gcggccggga attcgtcgac tggatccagt accgaggaga tctgcgccgc 1021 gatcgccggc gcgccagatc tcaagcttaa ctagctagcg gaccgacgcg tacgcggccg 1081 ctcgagcaga aactcatctc agaagaggat ctggcagcaa atgatatcct ggattacaag 1141 gatgacgacg ataaggttta aacggccggc cgcggtcata gctgtttcct gaacagatcc 1201 cgggtggcat ccctgtgacc cctccccagt gcctctcctg gccctggaag ttgccactcc 1261 agtgcccacc agccttgtcc taataaaatt aagttgcatc attttgtctg actaggtgtc 1321 cttctataat attatggggt ggaggggggt ggtatggagc aaggggcaag ttgggaagac 1381 aacctgtagg gcctgcgggg tctattggga accaagctgg agtgcagtgg cacaatcttg 1441 gctcactgca atctccgcct cctgggttca agcgattctc ctgcctcagc ctcccgagtt 1501 gttgggattc caggcatgca tgaccaggct cagctaattt ttgttttttt ggtagagacg 1561 gggtttcacc atattggcca ggctggtctc caactcctaa tctcaggtga tctacccacc 1621 ttggcctccc aaattgctgg gattacaggc gtgaaccact gctcccttcc ctgtccttct 1681 gattttaaaa taactatacc agcaggagga cgtccagaca cagcataggc tacctggcca 1741 tgcccaaccg gtgggacatt tgagttgctt gcttggcact gtcctctcat gcgttgggtc 1801 cactcagtag atgcctgttg aattgggtac gcggccagcg gcgagcggta tcagctcact 1861 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag 1921 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata 1981 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc 2041 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg 2101 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc 2161 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg 2221 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc 2281 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga 2341 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg 2401 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa 2461 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg 2521 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt 2581 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat 2641 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct 2701 aaagtatata tgagtaacct gaggctatgg cagggcctgc cgccccgacg ttggctgcga 2761 gccctgggcc ttcacccgaa cttggggggt ggggtgggga aaaggaagaa acgcgggcgt 2821 attggcccca atggggtctc ggtggggtat cgacagagtg ccagccctgg gaccgaaccc 2881 cgcgtttatg aacaaacgac ccaacaccgt gcgttttatt ctgtcttttt attgccgtca 2941 tagcgcgggt tccttccggt attgtctcct tccgtgtttc agttagcctc cccctagggt 3001 gggcgaagaa ctccagcatg agatccccgc gctggaggat catccagccg gcgtcccgga 3061 aaacgattcc gaagcccaac ctttcataga aggcggcggt ggaatcgaaa tctcgtgatg 3121 gcaggttggg cgtcgcttgg tcggtcattt tcttcgaatt attcttcacc ggcatctgca 3181 tccggggtct tgaaggcgtg ctggtactcc acgatgccca gctcggtgtt gctgtgatcc 3241 tcctccacgc ggcggaaggc gaacatgggg cccccgttct gcaggatgct ggggtggatg 3301 gcgctcttga agtgcatgtg gctgtccacc acggagctgt agtagccgcc gtcgcgcagg 3361 ctgaaggtgc gggtgaagct gccatccaga tcgttatcgc ccatggggtg caggtgctcc 3421 acggtggcgt tgctgcggat gatcttgtcg gtgaagatca cgctgtcctc ggggaagccg 3481 gtgcccatca ccttgaagtc gccgatcacg cggccggcct cgtagcggta gctgaagctc 3541 acgtgcagca cgccgccgtc ctcgtacttc tcgatgcggg tgttggtgta gccgccgttg 3601 ttgatggcgt gcaggaaggg gttctcgtag ccgctggggt aggtgccgaa gtggtagaag 3661 ccgtagccca tcacgtggct cagcaggtag gggctgaagg tcagggcgcc tttggtgctc 3721 ttcatcttgt tggtcatgcg gccctgctcg ggggtgccct ctccgccgcc caccagctcg 3781 aactccacgc cgttcagggt gccggtgatg cggcactcga tctccatggc gggcaggccg 3841 ctctcgtcgc tctccatggt tgtggccata ttatcatcgt gtttttcaaa ggaaaaccac 3901 gtccccgtgg ttcggggggc ctagacgttt ttttaacctc gactaaacac atgtaaagca 3961 tgtgcaccga ggccccagat cagatcccat acaatggggt accttctggg catccttcag 4021 ccccttgttg aatacgcttg aggagagcca tttgactctt tccacaacta tccaactcac 4081 aacgtggcac tggggttgtg ccgcctttgc aggtgtatct tatacacgtg gcttttggcc 4141 gcagaggcac ctgtcgccag gtggggggtt ccgctgcctg caaagggtcg ctacagacgt 4201 tgtttgtctt caagaagctt ccagaggaac tgcttccttc acgacattca acagaccttg 4261 cattcctttg gcgagagggg aaagacccct aggaatgctc gtcaagaaga cagggccagg 4321 tttccgggcc ctcacattgc caaaagacgg caatatggtg gaaaataaca tatagacaaa 4381 cgcacaccgg ccttattcca agcggcttcg gccagtaacg ttaggggggg gggcggaatt 4441 cgaaccccag agtcccgctc agaagaactc gtcaagaagg cgatagaagg cgatgcgctg 4501 cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag 4561 ctcttcagca atatcacggg tagccaacgc tatgtcctga tagcgatccg ccacacccag 4621 ccggccacag tcgatgaatc cagaaaagcg gccattttcc accatgatat tcggcaagca 4681 ggcatcgcca tgggtcacga cgagatcctc gccgtcgggc atgctcgcct tgagcctggc 4741 gaacagttcg gctggcgcga gcccctgatg ctcttcgtcc agatcatcct gatcgacaag 4801 accggcttcc atccgagtac gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg 4861 gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca tcagccatga tggatacttt 4921 ctcggcagga gcaaggtgag atgacaggag atcctgcccc ggcacttcgc ccaatagcag 4981 ccagtccctt cccgcttcag tgacaacgtc gagcacagct gcgcaaggaa cgcccgtcgt 5041 ggccagccac gatagccgcg ctgcctcgtc ttgcagttca ttcagggcac cggacaggtc 5101 ggtcttgaca aaaagaaccg ggcgcccctg cgctgacagc cggaacacgg cggcatcaga 5161 gcagccgatt gtctgttgtg cccagtcata gccgaatagc ctctccaccc aagcggccgg 5221 agaacctgcg tgcaatccat cttgttcaat catgcgaaac gatcctcatc ctgtctcttg 5281 atcgatcttt gcaaaagcct aggcctccaa aaaagcctcc tcactacttc tggaatagct 5341 cagaggccga ggcggcctcg gcctctgcat aaataaaaaa aattagtcag ccatggggcg 5401 gagaatgggc ggaactgggc ggagttaggg gcgggatggg cggagttagg ggcgggacta 5461 tggttgctga ctaattgaga tgcatgcttt gcatacttct gcctgctggg gagcctgggg 5521 actttccaca cctggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg 5581 gggagcctgg ggactttcca caccctaact gacacacatt ccacagctgg ttctttccgc 5641 ctcaggactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag 5701 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc 5761 ccgaaaagtg ccacctgacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt 5821 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt 5881 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc 5941 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga 6001 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc 6061 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt 6121 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct 6181 gatttaacaa aaatttaacg cgaattttaa caaaatatt //