LOCUS       .                      10186 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     rep_origin      1529..3318
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
                     /note="color: #ffff00"
                     /label="oriP"
     CDS             8409..9446
                     /codon_start=1
                     /note="confers resistance to hygromycin"
                     /note="color: #ccffcc"
                     /product="hygromycin B phosphotransferase"
                     /transl_table=1
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN*"
                     /label="HygR"
     CDS             6171..7031
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ccffcc"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      7202..7790
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /label="ori"
     enhancer        4..383
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
                     /label="CMV enhancer"
     promoter        384..587
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /note="color: #ffffff"
                     /label="CMV promoter"
     promoter        8221..8366
                     /note="herpes simplex virus thymidine kinase promoter"
                     /note="color: #ffffff"
                     /label="HSV TK promoter"
     polyA_signal    672..806
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
                     /label="SV40 poly(A) signal"
     promoter        6066..6170
                     /gene="bla"
                     /label="AmpR promoter"
                     /note="color: #ffffff"
     polyA_signal    9488..9536
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
                     /note="color: #a6acb3"
                     /label="HSV TK poly(A) signal"
     misc_feature    620..676
                     /note="multiple cloning site"
                     /note="color: #99ccff"
                     /label="MCS"
     CDS             complement(3620..5545)
                     /codon_start=1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita
                     et al., 2013)"
                     /note="color: #993366"
                     /product="Epstein-Barr nuclear antigen 1, also known as
                     EBNA-1"
                     /protein_id=""
                     /transl_table=1
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE*"
                     /label="EBNA1"
ORIGIN
        1 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtccgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttac gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtgatgc ggttttggca gtacaccaat gggcgtggat
      421 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
      481 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc ccgttgacgc
      541 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc
      601 gtcagatctc tagaagctgg gtaccagctg ctagcaagct tgctagcggc cgctcgaggc
      661 cggcaaggcc ggatccagac atgataagat acattgatga gtttggacaa accacaacta
      721 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
      781 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg
      841 ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg
      901 ctgattatga tccggctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
      961 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
     1021 tcaggcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgaggtcga ctctagagga
     1081 tcgatgcccc gccccggacg aactaaacct gactacgaca tctctgcccc ttcttcgcgg
     1141 ggcagtgcat gtaatccctt cagttggttg gtacaacttg ccaactgggc cctgttccac
     1201 atgtgacacg gggggggacc aaacacaaag gggttctctg actgtagttg acatccttat
     1261 aaatggatgt gcacatttgc caacactgag tggctttcat cctggagcag actttgcagt
     1321 ctgtggactg caacacaaca ttgcctttat gtgtaactct tggctgaagc tcttacacca
     1381 atgctggggg acatgtacct cccaggggcc caggaagact acgggaggct acaccaacgt
     1441 caatcagagg ggcctgtgta gctaccgata agcggaccct caagagggca ttagcaatag
     1501 tgtttataag gcccccttgt taaccctaaa cgggtagcat atgcttcccg ggtagtagta
     1561 tatactatcc agactaaccc taattcaata gcatatgtta cccaacggga agcatatgct
     1621 atcgaattag ggttagtaaa agggtcctaa ggaacagcga tatctcccac cccatgagct
     1681 gtcacggttt tatttacatg gggtcaggat tccacgaggg tagtgaacca ttttagtcac
     1741 aagggcagtg gctgaagatc aaggagcggg cagtgaactc tcctgaatct tcgcctgctt
     1801 cttcattctc cttcgtttag ctaatagaat aactgctgag ttgtgaacag taaggtgtat
     1861 gtgaggtgct cgaaaacaag gtttcaggtg acgcccccag aataaaattt ggacgggggg
     1921 ttcagtggtg gcattgtgct atgacaccaa tataaccctc acaaacccct tgggcaataa
     1981 atactagtgt aggaatgaaa cattctgaat atctttaaca atagaaatcc atggggtggg
     2041 gacaagccgt aaagactgga tgtccatctc acacgaattt atggctatgg gcaacacata
     2101 atcctagtgc aatatgatac tggggttatt aagatgtgtc ccaggcaggg accaagacag
     2161 gtgaaccatg ttgttacact ctatttgtaa caaggggaaa gagagtggac gccgacagca
     2221 gcggactcca ctggttgtct ctaacacccc cgaaaattaa acggggctcc acgccaatgg
     2281 ggcccataaa caaagacaag tggccactct tttttttgaa attgtggagt gggggcacgc
     2341 gtcagccccc acacgccgcc ctgcggtttt ggactgtaaa ataagggtgt aataacttgg
     2401 ctgattgtaa ccccgctaac cactgcggtc aaaccacttg cccacaaaac cactaatggc
     2461 accccgggga atacctgcat aagtaggtgg gcgggccaag ataggggcgc gattgctgcg
     2521 atctggagga caaattacac acacttgcgc ctgagcgcca agcacagggt tgttggtcct
     2581 catattcacg aggtcgctga gagcacggtg ggctaatgtt gccatgggta gcatatacta
     2641 cccaaatatc tggatagcat atgctatcct aatctatatc tgggtagcat aggctatcct
     2701 aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct
     2761 aatttatatc tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct
     2821 aatctatatc tgggtagtat atgctatcct aatctgtatc cgggtagcat atgctatcct
     2881 aatagagatt agggtagtat atgctatcct aatttatatc tgggtagcat atactaccca
     2941 aatatctgga tagcatatgc tatcctaatc tatatctggg tagcatatgc tatcctaatc
     3001 tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc tatcctaatc
     3061 tatatctggg tagtatatgc tatcctaatt tatatctggg tagcataggc tatcctaatc
     3121 tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc tatcctaatc
     3181 tgtatccggg tagcatatgc tatcctcatg catatacagt cagcatatga tacccagtag
     3241 tagagtggga gtgctatcct ttgcatatgc cgccacctcc caagggggcg tgaattttcg
     3301 ctgcttgtcc ttttcctgct ggttgctccc attcttaggt gaatttaagg aggccaggct
     3361 aaagccgtcg catgtctgat tgctcaccag gtaaatgtcg ctaatgtttt ccaacgcgag
     3421 aaggtgttga gcgcggagct gagtgacgtg acaacatggg tatgcccaat tgccccatgt
     3481 tgggaggacg aaaatggtga caagacagat ggccagaaat acaccaacag cacgcatgat
     3541 gtctactggg gatttattct ttagtgcggg ggaatacacg gcttttaata cgattgaggg
     3601 cgtctcctaa caagttacat cactcctgcc cttcctcacc ctcatctcca tcacctcctt
     3661 catctccgtc atctccgtca tcaccctccg cggcagcccc ttccaccata ggtggaaacc
     3721 agggaggcaa atctactcca tcgtcaaagc tgcacacagt caccctgata ttgcaggtag
     3781 gagcgggctt tgtcataaca aggtccttaa tcgcatcctt caaaacctca gcaaatatat
     3841 gagtttgtaa aaagaccatg aaataacaga caatggactc ccttagcggg ccaggttgtg
     3901 ggccgggtcc aggggccatt ccaaagggga gacgactcaa tggtgtaaga cgacattgtg
     3961 gaatagcaag ggcagttcct cgccttaggt tgtaaaggga ggtcttacta cctccatata
     4021 cgaacacacc ggcgacccaa gttccttcgt cggtagtcct ttctacgtga ctcctagcca
     4081 ggagagctct taaaccttct gcaatgttct caaatttcgg gttggaacct ccttgaccac
     4141 gatgctttcc aaaccaccct ccttttttgc gcctgcctcc atcaccctga ccccggggtc
     4201 cagtgcttgg gccttctcct gggtcatctg cggggccctg ctctatcgct cccgggggca
     4261 cgtcaggctc accatctggg ccaccttctt ggtggtattc aaaataatcg gcttccccta
     4321 cagggtggaa aaatggcctt ctacctggag ggggcctgcg cggtggagac ccggatgatg
     4381 atgactgact actgggactc ctgggcctct tttctccacg tccacgacct ctccccctgg
     4441 ctctttcacg acttcccccc ctggctcttt cacgtcctct accccggcgg cctccactac
     4501 ctcctcgacc ccggcctcca ctacctcctc gaccccggcc tccactgcct cctcgacccc
     4561 ggcctccacc tcctgctcct gcccctcctg ctcctgcccc tcctcctgct cctgcccctc
     4621 ctgcccctcc tgctcctgcc cctcctgccc ctcctgctcc tgcccctcct gcccctcctg
     4681 ctcctgcccc tcctgcccct cctcctgctc ctgcccctcc tgcccctcct cctgctcctg
     4741 cccctcctgc ccctcctgct cctgcccctc ctgcccctcc tgctcctgcc cctcctgccc
     4801 ctcctgctcc tgcccctcct gctcctgccc ctcctgctcc tgcccctcct gctcctgccc
     4861 ctcctgcccc tcctgcccct cctcctgctc ctgcccctcc tgctcctgcc cctcctgccc
     4921 ctcctgcccc tcctgctcct gcccctcctc ctgctcctgc ccctcctgcc cctcctgccc
     4981 ctcctcctgc tcctgcccct cctgcccctc ctcctgctcc tgcccctcct cctgctcctg
     5041 cccctcctgc ccctcctgcc cctcctcctg ctcctgcccc tcctgcccct cctcctgctc
     5101 ctgcccctcc tcctgctcct gcccctcctg cccctcctgc ccctcctcct gctcctgccc
     5161 ctcctcctgc tcctgcccct cctgcccctc ctgcccctcc tgcccctcct cctgctcctg
     5221 cccctcctcc tgctcctgcc cctcctgctc ctgcccctcc cgctcctgct cctgctcctg
     5281 ttccaccgtg ggtccctttg cagccaatgc aacttggacg tttttggggt ctccggacac
     5341 catctctatg tcttggccct gatcctgagc cgcccggggc tcctggtctt ccgcctcctc
     5401 gtcctcgtcc tcttccccgt cctcgtccat ggttatcacc ccctcttctt tgaggtccac
     5461 tgccgccgga gccttctggt ccagatgtgt ctcccttctc tcctaggcca tttccaggtc
     5521 ctgtacctgg cccctcgtca gacatgattc acactaaaag agatcaatag acatctttat
     5581 tagacgacgc tcagtgaata cagggagtgc agactcctgc cccctccaac agccccccca
     5641 ccctcatccc cttcatggtc gctgtcagac agatccaggt ctgaaaattc cccatcctcc
     5701 gaaccatcct cgtcctcatc accaattact cgcagcccgg aaaactcccg ctgaacatcc
     5761 tcaagatttg cgtcctgagc ctcaagccag gcctcaaatt cctcgtcccc ctttttgctg
     5821 gacggtaggg atggggattc tcgggacccc tcctcttcct cttcaaggtc accagacaga
     5881 gatgctactg gggcaacgga agaaaagctg ggtgcggcct gtgaggatca gcttatcgat
     5941 gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt
     6001 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
     6061 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     6121 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     6181 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     6241 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     6301 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     6361 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg
     6421 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     6481 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     6541 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     6601 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     6661 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa
     6721 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     6781 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     6841 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     6901 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     6961 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     7021 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     7081 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     7141 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     7201 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     7261 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     7321 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     7381 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     7441 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     7501 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     7561 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     7621 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     7681 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     7741 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     7801 acgcggcctt tttacggttc ctggcctttt gctggccttg aagctgtccc tgatggtcgt
     7861 catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga
     7921 gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc
     7981 ccagcgcgtc ggccccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga
     8041 tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc
     8101 aattcttgga gtggtgaatc cgttagcgag gtgccgccct gcttcatccc cgtggcccgt
     8161 tgctcgcgtt tgctggcggt gtccccggaa gaaatatatt tgcatgtctt tagttctatg
     8221 atgacacaaa ccccgcccag cgtcttgtca ttggcgaatt cgaacacgca gatgcagtcg
     8281 gggcggcgcg gtccgaggtc cacttcgcat attaaggtga cgcgtgtggc ctcgaacacc
     8341 gagcgaccct gcagcgaccc gcttaacagc gtcaacagcg tgccgcagat cccggggggc
     8401 aatgagatat gaaaaagcct gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa
     8461 agttcgacag cgtctccgac ctgatgcagc tctcggaggg cgaagaatct cgtgctttca
     8521 gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct
     8581 acaaagatcg ttatgtttat cggcactttg catcggccgc gctcccgatt ccggaagtgc
     8641 ttgacattgg ggaattcagc gagagcctga cctattgcat ctcccgccgt gcacagggtg
     8701 tcacgttgca agacctgcct gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg
     8761 ccatggatgc gatcgctgcg gccgatctta gccagacgag cgggttcggc ccattcggac
     8821 cgcaaggaat cggtcaatac actacatggc gtgatttcat atgcgcgatt gctgatcccc
     8881 atgtgtatca ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc
     8941 tcgatgagct gatgctttgg gccgaggact gccccgaagt ccggcacctc gtgcacgcgg
     9001 atttcggctc caacaatgtc ctgacggaca atggccgcat aacagcggtc attgactgga
     9061 gcgaggcgat gttcggggat tcccaatacg aggtcgccaa catcttcttc tggaggccgt
     9121 ggttggcttg tatggagcag cagacgcgct acttcgagcg gaggcatccg gagcttgcag
     9181 gatcgccgcg gctccgggcg tatatgctcc gcattggtct tgaccaactc tatcagagct
     9241 tggttgacgg caatttcgat gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc
     9301 gatccggagc cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga
     9361 ccgatggctg tgtagaagta ctcgccgata gtggaaaccg acgccccagc actcgtccgg
     9421 atcgggagat gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc
     9481 gctatgacgg caataaaaag acagaataaa acgcacgggt gttgggtcgt ttgttcataa
     9541 acgcggggtt cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg
     9601 ccaatacgcc cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc
     9661 agggctcgca gccaacgtcg gggcggcagg ccctgccata gccactggcc ccgtgggtta
     9721 gggacggggt cccccatggg gaatggttta tggttcgtgg gggttattat tttgggcgtt
     9781 gcgtggggtc aggtccacga ctggactgag cagacagacc catggttttt ggatggcctg
     9841 ggcatggacc gcatgtactg gcgcgacacg aacaccgggc gtctgtggct gccaaacacc
     9901 cccgaccccc aaaaaccacc gcgcggattt ctggcgtgcc aagctagtcg accaattctc
     9961 atgtttgaca gcttatcatc gcagatccgg gcaacgttgt tgccattgct gcaggcgcag
    10021 aactggtagg tatggaagat ctatacattg aatcaatatt ggcaattagc catattagtc
    10081 attggttata tagcataaat caatattggc tattggccat tgcatacgtt gtatctatat
    10141 cataatatgt acatttatat tggctcatgt ccaatatgac cgccat
//
