LOCUS       .                      12362 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             20..1825
                     /codon_start=1
                     /gene="uidA (or gusA) from E. coli"
                     /product="β-glucuronidase"
                     /transl_table=1
                     /translation="LRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAIA
                     VPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEVM
                     EHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHDF
                     FNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQV
                     VATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGQQFL
                     INHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDWA
                     DEHGIVVIDETAAVGFQLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARDK
                     NHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISDL
                     FDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMYT
                     DMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRKP
                     KSAAFLLQKRWTGMNFGEKPQQGGKQ"
                     /label="GUS"
                     /note="color: #993366"
     CDS             complement(9464..10489)
                     /codon_start=1
                     /note="confers resistance to hygromycin"
                     /note="color: #ccffcc"
                     /product="hygromycin B phosphotransferase"
                     /transl_table=1
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K*"
                     /label="HygR"
     CDS             complement(7929..8723)
                     /codon_start=1
                     /gene="aphA-3"
                     /note="confers resistance to kanamycin"
                     /note="color: #ccffcc"
                     /product="aminoglycoside phosphotransferase"
                     /protein_id=""
                     /transl_table=1
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF*"
                     /label="KanR"
     CDS             1829..2539
                     /codon_start=1
                     /gene="mgfp5"
                     /note="suitable for expression in plants due to removal of
                     a cryptic intron"
                     /note="color: #05fd14"
                     /product="GFP with folding enhancement mutations"
                     /protein_id=""
                     /transl_table=1
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKAN
                     FKTRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
                     /label="mgfp5"
     promoter        complement(10557..11234)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
                     /note="color: #ffffff"
                     /label="CaMV 35S promoter (enhanced)"
     CDS             4193..4822
                     /codon_start=1
                     /product="stability protein from Pseudomonas plasmid pVS1"
                     /transl_table=1
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI*"
                     /label="pVS1 StaA"
                     /note="color: #993366"
     rep_origin      complement(7254..7842)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /label="ori"
     promoter        12002..12347
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
                     /note="color: #ffffff"
                     /label="CaMV 35S promoter"
     terminator      2595..2847
                     /note="nopaline synthase terminator and poly(A) signal"
                     /note="color: #ffffff"
                     /label="NOS terminator"
     polyA_signal    9250..9424
                     /note="cauliflower mosaic virus polyadenylation signal"
                     /note="color: #a6acb3"
                     /label="CaMV poly(A) signal"
     misc_feature    6928..7068
                     /note="basis of mobility region from pBR322"
                     /note="color: #a6acb3"
                     /label="bom"
     misc_feature    11549..11605
                     /gene=""
                     /note="pUC18/19 multiple cloning site"
                     /note="color: #99ccff"
                     /label="MCS"
     promoter        11461..11491
                     /note="promoter for the E. coli lac operon"
                     /note="color: #ffffff"
                     /label="lac promoter"
     misc_feature    2869..2893
                     /note="right border repeat from nopaline C58 T-DNA"
                     /note="color: #ffe4c4"
                     /label="RB T-DNA repeat"
     misc_feature    9148..9172
                     /note="left border repeat from nopaline C58 T-DNA"
                     /note="color: #ffe4c4"
                     /label="LB T-DNA repeat"
     CDS             2546..2563
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /transl_table=1
                     /translation="HHHHHH"
                     /label="6xHis"
                     /note="color: #cc99b2"
     protein_bind    11499..11515
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
                     /label="lac operator"
     primer_bind     11523..11539
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /note="color: #a020f0"
                     /label="M13 rev"
     primer_bind     complement(11609..11625)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /note="color: #a020f0"
                     /label="M13 fwd"
     CDS             11535..11768
                     /codon_start=1
                     /gene="lacZ (truncated)"
                     /product="LacZα fragment of β-galactosidase"
                     /protein_id=""
                     /transl_table=1
                     /translation="MTMITNSSSVPGDPLESTCRHASLALAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEC*"
                     /label="lacZα"
                     /note="color: #993366"
     CDS             5251..6324
                     /codon_start=1
                     /note=""
                     /note="color: #993366"
                     /product="replication protein from Pseudomonas plasmid
                     pVS1"
                     /protein_id=""
                     /transl_table=1
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS*"
                     /label="pVS1 RepA"
     rep_origin      6390..6584
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /note="color: #ffff00"
                     /label="pVS1 oriV"
ORIGIN
        1 catggtagat ctgactagtt tacgtcctgt agaaacccca acccgtgaaa tcaaaaaact
       61 cgacggcctg tgggcattca gtctggatcg cgaaaactgt ggaattgatc agcgttggtg
      121 ggaaagcgcg ttacaagaaa gccgggcaat tgctgtgcca ggcagtttta acgatcagtt
      181 cgccgatgca gatattcgta attatgcggg caacgtctgg tatcagcgcg aagtctttat
      241 accgaaaggt tgggcaggcc agcgtatcgt gctgcgtttc gatgcggtca ctcattacgg
      301 caaagtgtgg gtcaataatc aggaagtgat ggagcatcag ggcggctata cgccatttga
      361 agccgatgtc acgccgtatg ttattgccgg gaaaagtgta cgtatcaccg tttgtgtgaa
      421 caacgaactg aactggcaga ctatcccgcc gggaatggtg attaccgacg aaaacggcaa
      481 gaaaaagcag tcttacttcc atgatttctt taactatgcc ggaatccatc gcagcgtaat
      541 gctctacacc acgccgaaca cctgggtgga cgatatcacc gtggtgacgc atgtcgcgca
      601 agactgtaac cacgcgtctg ttgactggca ggtggtggcc aatggtgatg tcagcgttga
      661 actgcgtgat gcggatcaac aggtggttgc aactggacaa ggcactagcg ggactttgca
      721 agtggtgaat ccgcacctct ggcaaccggg tgaaggttat ctctatgaac tgtgcgtcac
      781 agccaaaagc cagacagagt gtgatatcta cccgcttcgc gtcggcatcc ggtcagtggc
      841 agtgaagggc caacagttcc tgattaacca caaaccgttc tactttactg gctttggtcg
      901 tcatgaagat gcggacttac gtggcaaagg attcgataac gtgctgatgg tgcacgacca
      961 cgcattaatg gactggattg gggccaactc ctaccgtacc tcgcattacc cttacgctga
     1021 agagatgctc gactgggcag atgaacatgg catcgtggtg attgatgaaa ctgctgctgt
     1081 cggctttcag ctgtctttag gcattggttt cgaagcgggc aacaagccga aagaactgta
     1141 cagcgaagag gcagtcaacg gggaaactca gcaagcgcac ttacaggcga ttaaagagct
     1201 gatagcgcgt gacaaaaacc acccaagcgt ggtgatgtgg agtattgcca acgaaccgga
     1261 tacccgtccg caaggtgcac gggaatattt cgcgccactg gcggaagcaa cgcgtaaact
     1321 cgacccgacg cgtccgatca cctgcgtcaa tgtaatgttc tgcgacgctc acaccgatac
     1381 catcagcgat ctctttgatg tgctgtgcct gaaccgttat tacggatggt atgtccaaag
     1441 cggcgatttg gaaacggcag agaaggtact ggaaaaagaa cttctggcct ggcaggagaa
     1501 actgcatcag ccgattatca tcaccgaata cggcgtggat acgttagccg ggctgcactc
     1561 aatgtacacc gacatgtgga gtgaagagta tcagtgtgca tggctggata tgtatcaccg
     1621 cgtctttgat cgcgtcagcg ccgtcgtcgg tgaacaggta tggaatttcg ccgattttgc
     1681 gacctcgcaa ggcatattgc gcgttggcgg taacaagaaa gggatcttca ctcgcgaccg
     1741 caaaccgaag tcggcggctt ttctgctgca aaaacgctgg actggcatga acttcggtga
     1801 aaaaccgcag cagggaggca aacaagctag taaaggagaa gaacttttca ctggagttgt
     1861 cccaattctt gttgaattag atggtgatgt taatgggcac aaattttctg tcagtggaga
     1921 gggtgaaggt gatgcaacat acggaaaact tacccttaaa tttatttgca ctactggaaa
     1981 actacctgtt ccgtggccaa cacttgtcac tactttctct tatggtgttc aatgcttttc
     2041 aagataccca gatcatatga agcggcacga cttcttcaag agcgccatgc ctgagggata
     2101 cgtgcaggag aggaccatct tcttcaagga cgacgggaac tacaagacac gtgctgaagt
     2161 caagtttgag ggagacaccc tcgtcaacag gatcgagctt aagggaatcg atttcaagga
     2221 ggacggaaac atcctcggcc acaagttgga atacaactac aactcccaca acgtatacat
     2281 catggccgac aagcaaaaga acggcatcaa agccaacttc aagacccgcc acaacatcga
     2341 agacggcggc gtgcaactcg ctgatcatta tcaacaaaat actccaattg gcgatggccc
     2401 tgtcctttta ccagacaacc attacctgtc cacacaatct gccctttcga aagatcccaa
     2461 cgaaaagaga gaccacatgg tccttcttga gtttgtaaca gctgctggga ttacacatgg
     2521 catggatgaa ctatacaaag ctagccacca ccaccaccac cacgtgtgaa ttggtgacca
     2581 gctcgaattt ccccgatcgt tcaaacattt ggcaataaag tttcttaaga ttgaatcctg
     2641 ttgccggtct tgcgatgatt atcatataat ttctgttgaa ttacgttaag catgtaataa
     2701 ttaacatgta atgcatgacg ttatttatga gatgggtttt tatgattaga gtcccgcaat
     2761 tatacattta atacgcgata gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc
     2821 gcgcggtgtc atctatgtta ctagatcggg aattaaacta tcagtgtttg acaggatata
     2881 ttggcgggta aacctaagag aaaagagcgt ttattagaat aacggatatt taaaagggcg
     2941 tgaaaaggtt tatccgttcg tccatttgta tgtgcatgcc aaccacaggg ttcccctcgg
     3001 gatcaaagta ctttgatcca acccctccgc tgctatagtg cagtcggctt ctgacgttca
     3061 gtgcagccgt cttctgaaaa cgacatgtcg cacaagtcct aagttacgcg acaggctgcc
     3121 gccctgccct tttcctggcg ttttcttgtc gcgtgtttta gtcgcataaa gtagaatact
     3181 tgcgactaga accggagaca ttacgccatg aacaagagcg ccgccgctgg cctgctgggc
     3241 tatgcccgcg tcagcaccga cgaccaggac ttgaccaacc aacgggccga actgcacgcg
     3301 gccggctgca ccaagctgtt ttccgagaag atcaccggca ccaggcgcga ccgcccggag
     3361 ctggccagga tgcttgacca cctacgccct ggcgacgttg tgacagtgac caggctagac
     3421 cgcctggccc gcagcacccg cgacctactg gacattgccg agcgcatcca ggaggccggc
     3481 gcgggcctgc gtagcctggc agagccgtgg gccgacacca ccacgccggc cggccgcatg
     3541 gtgttgaccg tgttcgccgg cattgccgag ttcgagcgtt ccctaatcat cgaccgcacc
     3601 cggagcgggc gcgaggccgc caaggcccga ggcgtgaagt ttggcccccg ccctaccctc
     3661 accccggcac agatcgcgca cgcccgcgag ctgatcgacc aggaaggccg caccgtgaaa
     3721 gaggcggctg cactgcttgg cgtgcatcgc tcgaccctgt accgcgcact tgagcgcagc
     3781 gaggaagtga cgcccaccga ggccaggcgg cgcggtgcct tccgtgagga cgcattgacc
     3841 gaggccgacg ccctggcggc cgccgagaat gaacgccaag aggaacaagc atgaaaccgc
     3901 accaggacgg ccaggacgaa ccgtttttca ttaccgaaga gatcgaggcg gagatgatcg
     3961 cggccgggta cgtgttcgag ccgcccgcgc acgtctcaac cgtgcggctg catgaaatcc
     4021 tggccggttt gtctgatgcc aagctggcgg cctggccggc cagcttggcc gctgaagaaa
     4081 ccgagcgccg ccgtctaaaa aggtgatgtg tatttgagta aaacagcttg cgtcatgcgg
     4141 tcgctgcgta tatgatgcga tgagtaaata aacaaatacg caaggggaac gcatgaaggt
     4201 tatcgctgta cttaaccaga aaggcgggtc aggcaagacg accatcgcaa cccatctagc
     4261 ccgcgccctg caactcgccg gggccgatgt tctgttagtc gattccgatc cccagggcag
     4321 tgcccgcgat tgggcggccg tgcgggaaga tcaaccgcta accgttgtcg gcatcgaccg
     4381 cccgacgatt gaccgcgacg tgaaggccat cggccggcgc gacttcgtag tgatcgacgg
     4441 agcgccccag gcggcggact tggctgtgtc cgcgatcaag gcagccgact tcgtgctgat
     4501 tccggtgcag ccaagccctt acgacatatg ggccaccgcc gacctggtgg agctggttaa
     4561 gcagcgcatt gaggtcacgg atggaaggct acaagcggcc tttgtcgtgt cgcgggcgat
     4621 caaaggcacg cgcatcggcg gtgaggttgc cgaggcgctg gccgggtacg agctgcccat
     4681 tcttgagtcc cgtatcacgc agcgcgtgag ctacccaggc actgccgccg ccggcacaac
     4741 cgttcttgaa tcagaacccg agggcgacgc tgcccgcgag gtccaggcgc tggccgctga
     4801 aattaaatca aaactcattt gagttaatga ggtaaagaga aaatgagcaa aagcacaaac
     4861 acgctaagtg ccggccgtcc gagcgcacgc agcagcaagg ctgcaacgtt ggccagcctg
     4921 gcagacacgc cagccatgaa gcgggtcaac tttcagttgc cggcggagga tcacaccaag
     4981 ctgaagatgt acgcggtacg ccaaggcaag accattaccg agctgctatc tgaatacatc
     5041 gcgcagctac cagagtaaat gagcaaatga ataaatgagt agatgaattt tagcggctaa
     5101 aggaggcggc atggaaaatc aagaacaacc aggcaccgac gccgtggaat gccccatgtg
     5161 tggaggaacg ggcggttggc caggcgtaag cggctgggtt gtctgccggc cctgcaatgg
     5221 cactggaacc cccaagcccg aggaatcggc gtgagcggtc gcaaaccatc cggcccggta
     5281 caaatcggcg cggcgctggg tgatgacctg gtggagaagt tgaaggccgc gcaggccgcc
     5341 cagcggcaac gcatcgaggc agaagcacgc cccggtgaat cgtggcaagc ggccgctgat
     5401 cgaatccgca aagaatcccg gcaaccgccg gcagccggtg cgccgtcgat taggaagccg
     5461 cccaagggcg acgagcaacc agattttttc gttccgatgc tctatgacgt gggcacccgc
     5521 gatagtcgca gcatcatgga cgtggccgtt ttccgtctgt cgaagcgtga ccgacgagct
     5581 ggcgaggtga tccgctacga gcttccagac gggcacgtag aggtttccgc agggccggcc
     5641 ggcatggcca gtgtgtggga ttacgacctg gtactgatgg cggtttccca tctaaccgaa
     5701 tccatgaacc gataccggga agggaaggga gacaagcccg gccgcgtgtt ccgtccacac
     5761 gttgcggacg tactcaagtt ctgccggcga gccgatggcg gaaagcagaa agacgacctg
     5821 gtagaaacct gcattcggtt aaacaccacg cacgttgcca tgcagcgtac gaagaaggcc
     5881 aagaacggcc gcctggtgac ggtatccgag ggtgaagcct tgattagccg ctacaagatc
     5941 gtaaagagcg aaaccgggcg gccggagtac atcgagatcg agctagctga ttggatgtac
     6001 cgcgagatca cagaaggcaa gaacccggac gtgctgacgg ttcaccccga ttactttttg
     6061 atcgatcccg gcatcggccg ttttctctac cgcctggcac gccgcgccgc aggcaaggca
     6121 gaagccagat ggttgttcaa gacgatctac gaacgcagtg gcagcgccgg agagttcaag
     6181 aagttctgtt tcaccgtgcg caagctgatc gggtcaaatg acctgccgga gtacgatttg
     6241 aaggaggagg cggggcaggc tggcccgatc ctagtcatgc gctaccgcaa cctgatcgag
     6301 ggcgaagcat ccgccggttc ctaatgtacg gagcagatgc tagggcaaat tgccctagca
     6361 ggggaaaaag gtcgaaaagg tctctttcct gtggatagca cgtacattgg gaacccaaag
     6421 ccgtacattg ggaaccggaa cccgtacatt gggaacccaa agccgtacat tgggaaccgg
     6481 tcacacatgt aagtgactga tataaaagag aaaaaaggcg atttttccgc ctaaaactct
     6541 ttaaaactta ttaaaactct taaaacccgc ctggcctgtg cataactgtc tggccagcgc
     6601 acagccgaag agctgcaaaa agcgcctacc cttcggtcgc tgcgctccct acgccccgcc
     6661 gcttcgcgtc ggcctatcgc ggccgctggc cgctcaaaaa tggctggcct acggccaggc
     6721 aatctaccag ggcgcggaca agccgcgccg tcgccactcg accgccggcg cccacatcaa
     6781 ggcaccctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
     6841 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
     6901 gtcagcgggt gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg
     6961 agtgtatact ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg
     7021 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct
     7081 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     7141 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     7201 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     7261 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     7321 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     7381 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     7441 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     7501 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     7561 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     7621 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     7681 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     7741 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     7801 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     7861 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgcat
     7921 tctaggtact aaaacaattc atccagtaaa atataatatt ttattttctc ccaatcaggc
     7981 ttgatcccca gtaagtcaaa aaatagctcg acatactgtt cttccccgat atcctccctg
     8041 atcgaccgga cgcagaaggc aatgtcatac cacttgtccg ccctgccgct tctcccaaga
     8101 tcaataaagc cacttacttt gccatctttc acaaagatgt tgctgtctcc caggtcgccg
     8161 tgggaaaaga caagttcctc ttcgggcttt tccgtcttta aaaaatcata cagctcgcgc
     8221 ggatctttaa atggagtgtc ttcttcccag ttttcgcaat ccacatcggc cagatcgtta
     8281 ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc tattcgtata gggacaatcc
     8341 gatatgtcga tggagtgaaa gagcctgatg cactccgcat acagctcgat aatcttttca
     8401 gggctttgtt catcttcata ctcttccgag caaaggacgc catcggcctc actcatgagc
     8461 agattgctcc agccatcatg ccgttcaaag tgcaggacct ttggaacagg cagctttcct
     8521 tccagccata gcatcatgtc cttttcccgt tccacatcat aggtggtccc tttataccgg
     8581 ctgtccgtca tttttaaata taggttttca ttttctccca ccagcttata taccttagca
     8641 ggagacattc cttccgtatc ttttacgcag cggtattttt cgatcagttt tttcaattcc
     8701 ggtgatattc tcattttagc catttattat ttccttcctc ttttctacag tatttaaaga
     8761 taccccaaga agctaattat aacaagacga actccaattc actgttcctt gcattctaaa
     8821 accttaaata ccagaaaaca gctttttcaa agttgttttc aaagttggcg tataacatag
     8881 tatcgacgga gccgattttg aaaccgcggt gatcacaggc agcaacgctc tgtcatcgtt
     8941 acaatcaaca tgctaccctc cgcgagatca tccgtgtttc aaacccggca gcttagttgc
     9001 cgttcttccg aatagcatcg gtaacatgag caaagtctgc cgccttacaa cggctctccc
     9061 gctgacgccg tcccggactg atgggctgcc tgtatcgagt ggtgattttg tgccgagctg
     9121 ccggtcgggg agctgttggc tggctggtgg caggatatat tgtggtgtaa acaaattgac
     9181 gcttagacaa cttaataaca cattgcggac gtttttaatg tactgaatta acgccgaatt
     9241 aattcggggg atctggattt tagtactgga ttttggtttt aggaattaga aattttattg
     9301 atagaagtat tttacaaata caaatacata ctaagggttt cttatatgct caacacatga
     9361 gcgaaaccct ataggaaccc taattccctt atctgggaac tactcacaca ttattatgga
     9421 gaaactcgag cttgtcgatc gacagatccg gtcggcatct actctatttc tttgccctcg
     9481 gacgagtgct ggggcgtcgg tttccactat cggcgagtac ttctacacag ccatcggtcc
     9541 agacggccgc gcttctgcgg gcgatttgtg tacgcccgac agtcccggct ccggatcgga
     9601 cgattgcgtc gcatcgaccc tgcgcccaag ctgcatcatc gaaattgccg tcaaccaagc
     9661 tctgatagag ttggtcaaga ccaatgcgga gcatatacgc ccggagtcgt ggcgatcctg
     9721 caagctccgg atgcctccgc tcgaagtagc gcgtctgctg ctccatacaa gccaaccacg
     9781 gcctccagaa gaagatgttg gcgacctcgt attgggaatc cccgaacatc gcctcgctcc
     9841 agtcaatgac cgctgttatg cggccattgt ccgtcaggac attgttggag ccgaaatccg
     9901 cgtgcacgag gtgccggact tcggggcagt cctcggccca aagcatcagc tcatcgagag
     9961 cctgcgcgac ggacgcactg acggtgtcgt ccatcacagt ttgccagtga tacacatggg
    10021 gatcagcaat cgcgcatatg aaatcacgcc atgtagtgta ttgaccgatt ccttgcggtc
    10081 cgaatgggcc gaacccgctc gtctggctaa gatcggccgc agcgatcgca tccatagcct
    10141 ccgcgaccgg ttgtagaaca gcgggcagtt cggtttcagg caggtcttgc aacgtgacac
    10201 cctgtgcacg gcgggagatg caataggtca ggctctcgct aaactcccca atgtcaagca
    10261 cttccggaat cgggagcgcg gccgatgcaa agtgccgata aacataacga tctttgtaga
    10321 aaccatcggc gcagctattt acccgcagga catatccacg ccctcctaca tcgaagctga
    10381 aagcacgaga ttcttcgccc tccgagagct gcatcaggtc ggagacgctg tcgaactttt
    10441 cgatcagaaa cttctcgaca gacgtcgcgg tgagttcagg ctttttcata tctcattgcc
    10501 ccccgggatc tgcgaaagct cgagagagat agatttgtag agagagactg gtgatttcag
    10561 cgtgtcctct ccaaatgaaa tgaacttcct tatatagagg aaggtcttgc gaaggatagt
    10621 gggattgtgc gtcatccctt acgtcagtgg agatatcaca tcaatccact tgctttgaag
    10681 acgtggttgg aacgtcttct ttttccacga tgctcctcgt gggtgggggt ccatctttgg
    10741 gaccactgtc ggcagaggca tcttgaacga tagcctttcc tttatcgcaa tgatggcatt
    10801 tgtaggtgcc accttccttt tctactgtcc ttttgatgaa gtgacagata gctgggcaat
    10861 ggaatccgag gaggtttccc gatattaccc tttgttgaaa agtctcaata gccctttggt
    10921 cttctgagac tgtatctttg atattcttgg agtagacgag agtgtcgtgc tccaccatgt
    10981 tatcacatca atccacttgc tttgaagacg tggttggaac gtcttctttt tccacgatgc
    11041 tcctcgtggg tgggggtcca tctttgggac cactgtcggc agaggcatct tgaacgatag
    11101 cctttccttt atcgcaatga tggcatttgt aggtgccacc ttccttttct actgtccttt
    11161 tgatgaagtg acagatagct gggcaatgga atccgaggag gtttcccgat attacccttt
    11221 gttgaaaagt ctcaatagcc ctttggtctt ctgagactgt atctttgata ttcttggagt
    11281 agacgagagt gtcgtgctcc accatgttgg caagctgctc tagccaatac gcaaaccgcc
    11341 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
    11401 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
    11461 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
    11521 cacaggaaac agctatgacc atgattacga attcgagctc ggtacccggg gatcctctag
    11581 agtcgacctg caggcatgca agcttggcac tggccgtcgt tttacaacgt cgtgactggg
    11641 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
    11701 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
    11761 aatgctagag cagcttgagc ttggatcaga ttgtcgtttc ccgccttcag tttagcttca
    11821 tggagtcaaa gattcaaata gaggacctaa cagaactcgc cgtaaagact ggcgaacagt
    11881 tcatacagag tctcttacga ctcaatgaca agaagaaaat cttcgtcaac atggtggagc
    11941 acgacacact tgtctactcc aaaaatatca aagatacagt ctcagaagac caaagggcaa
    12001 ttgagacttt tcaacaaagg gtaatatccg gaaacctcct cggattccat tgcccagcta
    12061 tctgtcactt tattgtgaag atagtggaaa aggaaggtgg ctcctacaaa tgccatcatt
    12121 gcgataaagg aaaggccatc gttgaagatg cctctgccga cagtggtccc aaagatggac
    12181 ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag
    12241 tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac tatccttcgc
    12301 aagacccttc ctctatataa ggaagttcat ttcatttgga gagaacacgg gggactcttg
    12361 ac
//
