LOCUS       .                      10550 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             complement(7652..8677)
                     /codon_start=1
                     /note="confers resistance to hygromycin"
                     /note="color: #ccffcc"
                     /product="hygromycin B phosphotransferase"
                     /transl_table=1
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K*"
                     /label="HygR"
     CDS             complement(6117..6911)
                     /codon_start=1
                     /gene="aphA-3"
                     /note="confers resistance to kanamycin"
                     /note="color: #ccffcc"
                     /product="aminoglycoside phosphotransferase"
                     /protein_id=""
                     /transl_table=1
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF*"
                     /label="KanR"
     CDS             17..727
                     /codon_start=1
                     /gene="mgfp5"
                     /note="suitable for expression in plants due to removal of
                     a cryptic intron"
                     /note="color: #05fd14"
                     /product="GFP with folding enhancement mutations"
                     /protein_id=""
                     /transl_table=1
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKAN
                     FKTRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
                     /label="mgfp5"
     promoter        complement(8745..9422)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
                     /note="color: #ffffff"
                     /label="CaMV 35S promoter (enhanced)"
     CDS             2381..3010
                     /codon_start=1
                     /product="stability protein from Pseudomonas plasmid pVS1"
                     /transl_table=1
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI*"
                     /label="pVS1 StaA"
                     /note="color: #993366"
     rep_origin      complement(5442..6030)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /label="ori"
     promoter        10190..10535
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
                     /note="color: #ffffff"
                     /label="CaMV 35S promoter"
     terminator      783..1035
                     /note="nopaline synthase terminator and poly(A) signal"
                     /note="color: #ffffff"
                     /label="NOS terminator"
     polyA_signal    7438..7612
                     /note="cauliflower mosaic virus polyadenylation signal"
                     /note="color: #a6acb3"
                     /label="CaMV poly(A) signal"
     misc_feature    5116..5256
                     /note="basis of mobility region from pBR322"
                     /note="color: #a6acb3"
                     /label="bom"
     misc_feature    9737..9793
                     /gene=""
                     /note="pUC18/19 multiple cloning site"
                     /note="color: #99ccff"
                     /label="MCS"
     promoter        9649..9679
                     /note="promoter for the E. coli lac operon"
                     /note="color: #ffffff"
                     /label="lac promoter"
     misc_feature    1057..1081
                     /note="right border repeat from nopaline C58 T-DNA"
                     /note="color: #ffe4c4"
                     /label="RB T-DNA repeat"
     misc_feature    7336..7360
                     /note="left border repeat from nopaline C58 T-DNA"
                     /note="color: #ffe4c4"
                     /label="LB T-DNA repeat"
     CDS             734..751
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /transl_table=1
                     /translation="HHHHHH"
                     /label="6xHis"
                     /note="color: #cc99b2"
     protein_bind    9687..9703
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
                     /label="lac operator"
     primer_bind     9711..9727
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /note="color: #a020f0"
                     /label="M13 rev"
     primer_bind     complement(9797..9813)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /note="color: #a020f0"
                     /label="M13 fwd"
     CDS             9723..9956
                     /codon_start=1
                     /gene="lacZ (truncated)"
                     /product="LacZα fragment of β-galactosidase"
                     /protein_id=""
                     /transl_table=1
                     /translation="MTMITNSSSVPGDPLESTCRHASLALAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEC*"
                     /label="lacZα"
                     /note="color: #993366"
     CDS             3439..4512
                     /codon_start=1
                     /note=""
                     /note="color: #993366"
                     /product="replication protein from Pseudomonas plasmid
                     pVS1"
                     /protein_id=""
                     /transl_table=1
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS*"
                     /label="pVS1 RepA"
     rep_origin      4578..4772
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /note="color: #ffff00"
                     /label="pVS1 oriV"
ORIGIN
        1 catggtagat ctgactagta aaggagaaga acttttcact ggagttgtcc caattcttgt
       61 tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga
      121 tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc
      181 gtggccaaca cttgtcacta ctttctctta tggtgttcaa tgcttttcaa gatacccaga
      241 tcatatgaag cggcacgact tcttcaagag cgccatgcct gagggatacg tgcaggagag
      301 gaccatcttc ttcaaggacg acgggaacta caagacacgt gctgaagtca agtttgaggg
      361 agacaccctc gtcaacagga tcgagcttaa gggaatcgat ttcaaggagg acggaaacat
      421 cctcggccac aagttggaat acaactacaa ctcccacaac gtatacatca tggccgacaa
      481 gcaaaagaac ggcatcaaag ccaacttcaa gacccgccac aacatcgaag acggcggcgt
      541 gcaactcgct gatcattatc aacaaaatac tccaattggc gatggccctg tccttttacc
      601 agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg aaaagagaga
      661 ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca tggatgaact
      721 atacaaagct agccaccacc accaccacca cgtgtgaatt ggtgaccagc tcgaatttcc
      781 ccgatcgttc aaacatttgg caataaagtt tcttaagatt gaatcctgtt gccggtcttg
      841 cgatgattat catataattt ctgttgaatt acgttaagca tgtaataatt aacatgtaat
      901 gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta tacatttaat
      961 acgcgataga aaacaaaata tagcgcgcaa actaggataa attatcgcgc gcggtgtcat
     1021 ctatgttact agatcgggaa ttaaactatc agtgtttgac aggatatatt ggcgggtaaa
     1081 cctaagagaa aagagcgttt attagaataa cggatattta aaagggcgtg aaaaggttta
     1141 tccgttcgtc catttgtatg tgcatgccaa ccacagggtt cccctcggga tcaaagtact
     1201 ttgatccaac ccctccgctg ctatagtgca gtcggcttct gacgttcagt gcagccgtct
     1261 tctgaaaacg acatgtcgca caagtcctaa gttacgcgac aggctgccgc cctgcccttt
     1321 tcctggcgtt ttcttgtcgc gtgttttagt cgcataaagt agaatacttg cgactagaac
     1381 cggagacatt acgccatgaa caagagcgcc gccgctggcc tgctgggcta tgcccgcgtc
     1441 agcaccgacg accaggactt gaccaaccaa cgggccgaac tgcacgcggc cggctgcacc
     1501 aagctgtttt ccgagaagat caccggcacc aggcgcgacc gcccggagct ggccaggatg
     1561 cttgaccacc tacgccctgg cgacgttgtg acagtgacca ggctagaccg cctggcccgc
     1621 agcacccgcg acctactgga cattgccgag cgcatccagg aggccggcgc gggcctgcgt
     1681 agcctggcag agccgtgggc cgacaccacc acgccggccg gccgcatggt gttgaccgtg
     1741 ttcgccggca ttgccgagtt cgagcgttcc ctaatcatcg accgcacccg gagcgggcgc
     1801 gaggccgcca aggcccgagg cgtgaagttt ggcccccgcc ctaccctcac cccggcacag
     1861 atcgcgcacg cccgcgagct gatcgaccag gaaggccgca ccgtgaaaga ggcggctgca
     1921 ctgcttggcg tgcatcgctc gaccctgtac cgcgcacttg agcgcagcga ggaagtgacg
     1981 cccaccgagg ccaggcggcg cggtgccttc cgtgaggacg cattgaccga ggccgacgcc
     2041 ctggcggccg ccgagaatga acgccaagag gaacaagcat gaaaccgcac caggacggcc
     2101 aggacgaacc gtttttcatt accgaagaga tcgaggcgga gatgatcgcg gccgggtacg
     2161 tgttcgagcc gcccgcgcac gtctcaaccg tgcggctgca tgaaatcctg gccggtttgt
     2221 ctgatgccaa gctggcggcc tggccggcca gcttggccgc tgaagaaacc gagcgccgcc
     2281 gtctaaaaag gtgatgtgta tttgagtaaa acagcttgcg tcatgcggtc gctgcgtata
     2341 tgatgcgatg agtaaataaa caaatacgca aggggaacgc atgaaggtta tcgctgtact
     2401 taaccagaaa ggcgggtcag gcaagacgac catcgcaacc catctagccc gcgccctgca
     2461 actcgccggg gccgatgttc tgttagtcga ttccgatccc cagggcagtg cccgcgattg
     2521 ggcggccgtg cgggaagatc aaccgctaac cgttgtcggc atcgaccgcc cgacgattga
     2581 ccgcgacgtg aaggccatcg gccggcgcga cttcgtagtg atcgacggag cgccccaggc
     2641 ggcggacttg gctgtgtccg cgatcaaggc agccgacttc gtgctgattc cggtgcagcc
     2701 aagcccttac gacatatggg ccaccgccga cctggtggag ctggttaagc agcgcattga
     2761 ggtcacggat ggaaggctac aagcggcctt tgtcgtgtcg cgggcgatca aaggcacgcg
     2821 catcggcggt gaggttgccg aggcgctggc cgggtacgag ctgcccattc ttgagtcccg
     2881 tatcacgcag cgcgtgagct acccaggcac tgccgccgcc ggcacaaccg ttcttgaatc
     2941 agaacccgag ggcgacgctg cccgcgaggt ccaggcgctg gccgctgaaa ttaaatcaaa
     3001 actcatttga gttaatgagg taaagagaaa atgagcaaaa gcacaaacac gctaagtgcc
     3061 ggccgtccga gcgcacgcag cagcaaggct gcaacgttgg ccagcctggc agacacgcca
     3121 gccatgaagc gggtcaactt tcagttgccg gcggaggatc acaccaagct gaagatgtac
     3181 gcggtacgcc aaggcaagac cattaccgag ctgctatctg aatacatcgc gcagctacca
     3241 gagtaaatga gcaaatgaat aaatgagtag atgaatttta gcggctaaag gaggcggcat
     3301 ggaaaatcaa gaacaaccag gcaccgacgc cgtggaatgc cccatgtgtg gaggaacggg
     3361 cggttggcca ggcgtaagcg gctgggttgt ctgccggccc tgcaatggca ctggaacccc
     3421 caagcccgag gaatcggcgt gagcggtcgc aaaccatccg gcccggtaca aatcggcgcg
     3481 gcgctgggtg atgacctggt ggagaagttg aaggccgcgc aggccgccca gcggcaacgc
     3541 atcgaggcag aagcacgccc cggtgaatcg tggcaagcgg ccgctgatcg aatccgcaaa
     3601 gaatcccggc aaccgccggc agccggtgcg ccgtcgatta ggaagccgcc caagggcgac
     3661 gagcaaccag attttttcgt tccgatgctc tatgacgtgg gcacccgcga tagtcgcagc
     3721 atcatggacg tggccgtttt ccgtctgtcg aagcgtgacc gacgagctgg cgaggtgatc
     3781 cgctacgagc ttccagacgg gcacgtagag gtttccgcag ggccggccgg catggccagt
     3841 gtgtgggatt acgacctggt actgatggcg gtttcccatc taaccgaatc catgaaccga
     3901 taccgggaag ggaagggaga caagcccggc cgcgtgttcc gtccacacgt tgcggacgta
     3961 ctcaagttct gccggcgagc cgatggcgga aagcagaaag acgacctggt agaaacctgc
     4021 attcggttaa acaccacgca cgttgccatg cagcgtacga agaaggccaa gaacggccgc
     4081 ctggtgacgg tatccgaggg tgaagccttg attagccgct acaagatcgt aaagagcgaa
     4141 accgggcggc cggagtacat cgagatcgag ctagctgatt ggatgtaccg cgagatcaca
     4201 gaaggcaaga acccggacgt gctgacggtt caccccgatt actttttgat cgatcccggc
     4261 atcggccgtt ttctctaccg cctggcacgc cgcgccgcag gcaaggcaga agccagatgg
     4321 ttgttcaaga cgatctacga acgcagtggc agcgccggag agttcaagaa gttctgtttc
     4381 accgtgcgca agctgatcgg gtcaaatgac ctgccggagt acgatttgaa ggaggaggcg
     4441 gggcaggctg gcccgatcct agtcatgcgc taccgcaacc tgatcgaggg cgaagcatcc
     4501 gccggttcct aatgtacgga gcagatgcta gggcaaattg ccctagcagg ggaaaaaggt
     4561 cgaaaaggtc tctttcctgt ggatagcacg tacattggga acccaaagcc gtacattggg
     4621 aaccggaacc cgtacattgg gaacccaaag ccgtacattg ggaaccggtc acacatgtaa
     4681 gtgactgata taaaagagaa aaaaggcgat ttttccgcct aaaactcttt aaaacttatt
     4741 aaaactctta aaacccgcct ggcctgtgca taactgtctg gccagcgcac agccgaagag
     4801 ctgcaaaaag cgcctaccct tcggtcgctg cgctccctac gccccgccgc ttcgcgtcgg
     4861 cctatcgcgg ccgctggccg ctcaaaaatg gctggcctac ggccaggcaa tctaccaggg
     4921 cgcggacaag ccgcgccgtc gccactcgac cgccggcgcc cacatcaagg caccctgcct
     4981 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
     5041 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
     5101 tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg
     5161 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata
     5221 ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact
     5281 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     5341 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     5401 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     5461 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     5521 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     5581 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     5641 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     5701 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     5761 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     5821 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     5881 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     5941 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     6001 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     6061 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgcattc taggtactaa
     6121 aacaattcat ccagtaaaat ataatatttt attttctccc aatcaggctt gatccccagt
     6181 aagtcaaaaa atagctcgac atactgttct tccccgatat cctccctgat cgaccggacg
     6241 cagaaggcaa tgtcatacca cttgtccgcc ctgccgcttc tcccaagatc aataaagcca
     6301 cttactttgc catctttcac aaagatgttg ctgtctccca ggtcgccgtg ggaaaagaca
     6361 agttcctctt cgggcttttc cgtctttaaa aaatcataca gctcgcgcgg atctttaaat
     6421 ggagtgtctt cttcccagtt ttcgcaatcc acatcggcca gatcgttatt cagtaagtaa
     6481 tccaattcgg ctaagcggct gtctaagcta ttcgtatagg gacaatccga tatgtcgatg
     6541 gagtgaaaga gcctgatgca ctccgcatac agctcgataa tcttttcagg gctttgttca
     6601 tcttcatact cttccgagca aaggacgcca tcggcctcac tcatgagcag attgctccag
     6661 ccatcatgcc gttcaaagtg caggaccttt ggaacaggca gctttccttc cagccatagc
     6721 atcatgtcct tttcccgttc cacatcatag gtggtccctt tataccggct gtccgtcatt
     6781 tttaaatata ggttttcatt ttctcccacc agcttatata ccttagcagg agacattcct
     6841 tccgtatctt ttacgcagcg gtatttttcg atcagttttt tcaattccgg tgatattctc
     6901 attttagcca tttattattt ccttcctctt ttctacagta tttaaagata ccccaagaag
     6961 ctaattataa caagacgaac tccaattcac tgttccttgc attctaaaac cttaaatacc
     7021 agaaaacagc tttttcaaag ttgttttcaa agttggcgta taacatagta tcgacggagc
     7081 cgattttgaa accgcggtga tcacaggcag caacgctctg tcatcgttac aatcaacatg
     7141 ctaccctccg cgagatcatc cgtgtttcaa acccggcagc ttagttgccg ttcttccgaa
     7201 tagcatcggt aacatgagca aagtctgccg ccttacaacg gctctcccgc tgacgccgtc
     7261 ccggactgat gggctgcctg tatcgagtgg tgattttgtg ccgagctgcc ggtcggggag
     7321 ctgttggctg gctggtggca ggatatattg tggtgtaaac aaattgacgc ttagacaact
     7381 taataacaca ttgcggacgt ttttaatgta ctgaattaac gccgaattaa ttcgggggat
     7441 ctggatttta gtactggatt ttggttttag gaattagaaa ttttattgat agaagtattt
     7501 tacaaataca aatacatact aagggtttct tatatgctca acacatgagc gaaaccctat
     7561 aggaacccta attcccttat ctgggaacta ctcacacatt attatggaga aactcgagct
     7621 tgtcgatcga cagatccggt cggcatctac tctatttctt tgccctcgga cgagtgctgg
     7681 ggcgtcggtt tccactatcg gcgagtactt ctacacagcc atcggtccag acggccgcgc
     7741 ttctgcgggc gatttgtgta cgcccgacag tcccggctcc ggatcggacg attgcgtcgc
     7801 atcgaccctg cgcccaagct gcatcatcga aattgccgtc aaccaagctc tgatagagtt
     7861 ggtcaagacc aatgcggagc atatacgccc ggagtcgtgg cgatcctgca agctccggat
     7921 gcctccgctc gaagtagcgc gtctgctgct ccatacaagc caaccacggc ctccagaaga
     7981 agatgttggc gacctcgtat tgggaatccc cgaacatcgc ctcgctccag tcaatgaccg
     8041 ctgttatgcg gccattgtcc gtcaggacat tgttggagcc gaaatccgcg tgcacgaggt
     8101 gccggacttc ggggcagtcc tcggcccaaa gcatcagctc atcgagagcc tgcgcgacgg
     8161 acgcactgac ggtgtcgtcc atcacagttt gccagtgata cacatgggga tcagcaatcg
     8221 cgcatatgaa atcacgccat gtagtgtatt gaccgattcc ttgcggtccg aatgggccga
     8281 acccgctcgt ctggctaaga tcggccgcag cgatcgcatc catagcctcc gcgaccggtt
     8341 gtagaacagc gggcagttcg gtttcaggca ggtcttgcaa cgtgacaccc tgtgcacggc
     8401 gggagatgca ataggtcagg ctctcgctaa actccccaat gtcaagcact tccggaatcg
     8461 ggagcgcggc cgatgcaaag tgccgataaa cataacgatc tttgtagaaa ccatcggcgc
     8521 agctatttac ccgcaggaca tatccacgcc ctcctacatc gaagctgaaa gcacgagatt
     8581 cttcgccctc cgagagctgc atcaggtcgg agacgctgtc gaacttttcg atcagaaact
     8641 tctcgacaga cgtcgcggtg agttcaggct ttttcatatc tcattgcccc ccgggatctg
     8701 cgaaagctcg agagagatag atttgtagag agagactggt gatttcagcg tgtcctctcc
     8761 aaatgaaatg aacttcctta tatagaggaa ggtcttgcga aggatagtgg gattgtgcgt
     8821 catcccttac gtcagtggag atatcacatc aatccacttg ctttgaagac gtggttggaa
     8881 cgtcttcttt ttccacgatg ctcctcgtgg gtgggggtcc atctttggga ccactgtcgg
     8941 cagaggcatc ttgaacgata gcctttcctt tatcgcaatg atggcatttg taggtgccac
     9001 cttccttttc tactgtcctt ttgatgaagt gacagatagc tgggcaatgg aatccgagga
     9061 ggtttcccga tattaccctt tgttgaaaag tctcaatagc cctttggtct tctgagactg
     9121 tatctttgat attcttggag tagacgagag tgtcgtgctc caccatgtta tcacatcaat
     9181 ccacttgctt tgaagacgtg gttggaacgt cttctttttc cacgatgctc ctcgtgggtg
     9241 ggggtccatc tttgggacca ctgtcggcag aggcatcttg aacgatagcc tttcctttat
     9301 cgcaatgatg gcatttgtag gtgccacctt ccttttctac tgtccttttg atgaagtgac
     9361 agatagctgg gcaatggaat ccgaggaggt ttcccgatat taccctttgt tgaaaagtct
     9421 caatagccct ttggtcttct gagactgtat ctttgatatt cttggagtag acgagagtgt
     9481 cgtgctccac catgttggca agctgctcta gccaatacgc aaaccgcctc tccccgcgcg
     9541 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
     9601 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
     9661 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
     9721 ctatgaccat gattacgaat tcgagctcgg tacccgggga tcctctagag tcgacctgca
     9781 ggcatgcaag cttggcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     9841 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     9901 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgctagagca
     9961 gcttgagctt ggatcagatt gtcgtttccc gccttcagtt tagcttcatg gagtcaaaga
    10021 ttcaaataga ggacctaaca gaactcgccg taaagactgg cgaacagttc atacagagtc
    10081 tcttacgact caatgacaag aagaaaatct tcgtcaacat ggtggagcac gacacacttg
    10141 tctactccaa aaatatcaaa gatacagtct cagaagacca aagggcaatt gagacttttc
    10201 aacaaagggt aatatccgga aacctcctcg gattccattg cccagctatc tgtcacttta
    10261 ttgtgaagat agtggaaaag gaaggtggct cctacaaatg ccatcattgc gataaaggaa
    10321 aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa agatggaccc ccacccacga
    10381 ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg
    10441 atatctccac tgacgtaagg gatgacgcac aatcccacta tccttcgcaa gacccttcct
    10501 ctatataagg aagttcattt catttggaga gaacacgggg gactcttgac
//
