LOCUS       .                       9111 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             complement(6642..7667)
                     /codon_start=1
                     /note="confers resistance to hygromycin"
                     /note="color: #ccffcc"
                     /product="hygromycin B phosphotransferase"
                     /transl_table=1
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K*"
                     /label="HygR"
     promoter        complement(7735..8412)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
                     /note="color: #ffffff"
                     /label="CaMV 35S promoter (enhanced)"
     CDS             complement(5139..5798)
                     /codon_start=1
                     /gene="cat"
                     /note="confers resistance to chloramphenicol"
                     /note="color: #ccffcc"
                     /product="chloramphenicol acetyltransferase"
                     /transl_table=1
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA*"
                     /label="CmR"
     CDS             1219..1848
                     /codon_start=1
                     /product="stability protein from Pseudomonas plasmid pVS1"
                     /transl_table=1
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI*"
                     /label="pVS1 StaA"
                     /note="color: #993366"
     rep_origin      complement(4280..4868)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /label="ori"
     polyA_signal    6428..6602
                     /note="cauliflower mosaic virus polyadenylation signal"
                     /note="color: #a6acb3"
                     /label="CaMV poly(A) signal"
     misc_feature    3954..4094
                     /note="basis of mobility region from pBR322"
                     /note="color: #a6acb3"
                     /label="bom"
     misc_feature    8727..8783
                     /gene=""
                     /note="pUC18/19 multiple cloning site"
                     /note="color: #99ccff"
                     /label="MCS"
     promoter        8639..8669
                     /note="promoter for the E. coli lac operon"
                     /note="color: #ffffff"
                     /label="lac promoter"
     misc_feature    6326..6350
                     /note="left border repeat from nopaline C58 T-DNA"
                     /note="color: #ffe4c4"
                     /label="LB T-DNA repeat"
     misc_feature    9006..9030
                     /note="right border repeat from nopaline C58 T-DNA"
                     /note="color: #ffe4c4"
                     /label="RB T-DNA repeat"
     protein_bind    8677..8693
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
                     /label="lac operator"
     primer_bind     8701..8717
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /note="color: #a020f0"
                     /label="M13 rev"
     primer_bind     complement(8787..8803)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /note="color: #a020f0"
                     /label="M13 fwd"
     CDS             2277..3350
                     /codon_start=1
                     /note=""
                     /note="color: #993366"
                     /product="replication protein from Pseudomonas plasmid
                     pVS1"
                     /protein_id=""
                     /transl_table=1
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS*"
                     /label="pVS1 RepA"
     CDS             8713..8946
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZα fragment of β-galactosidase"
                     /transl_table=1
                     /translation="MTMITNSSSVPGDPLESTCRHASLALAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEC*"
                     /label="lacZα"
                     /note="color: #993366"
     rep_origin      3416..3610
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /note="color: #ffff00"
                     /label="pVS1 oriV"
ORIGIN
        1 catgccaacc acagggttcc cctcgggatc aaagtacttt gatccaaccc ctccgctgct
       61 atagtgcagt cggcttctga cgttcagtgc agccgtcttc tgaaaacgac atgtcgcaca
      121 agtcctaagt tacgcgacag gctgccgccc tgcccttttc ctggcgtttt cttgtcgcgt
      181 gttttagtcg cataaagtag aatacttgcg actagaaccg gagacattac gccatgaaca
      241 agagcgccgc cgctggcctg ctgggctatg cccgcgtcag caccgacgac caggacttga
      301 ccaaccaacg ggccgaactg cacgcggccg gctgcaccaa gctgttttcc gagaagatca
      361 ccggcaccag gcgcgaccgc ccggagctgg ccaggatgct tgaccaccta cgccctggcg
      421 acgttgtgac agtgaccagg ctagaccgcc tggcccgcag cacccgcgac ctactggaca
      481 ttgccgagcg catccaggag gccggcgcgg gcctgcgtag cctggcagag ccgtgggccg
      541 acaccaccac gccggccggc cgcatggtgt tgaccgtgtt cgccggcatt gccgagttcg
      601 agcgttccct aatcatcgac cgcacccgga gcgggcgcga ggccgccaag gcccgaggcg
      661 tgaagtttgg cccccgccct accctcaccc cggcacagat cgcgcacgcc cgcgagctga
      721 tcgaccagga aggccgcacc gtgaaagagg cggctgcact gcttggcgtg catcgctcga
      781 ccctgtaccg cgcacttgag cgcagcgagg aagtgacgcc caccgaggcc aggcggcgcg
      841 gtgccttccg tgaggacgca ttgaccgagg ccgacgccct ggcggccgcc gagaatgaac
      901 gccaagagga acaagcatga aaccgcacca ggacggccag gacgaaccgt ttttcattac
      961 cgaagagatc gaggcggaga tgatcgcggc cgggtacgtg ttcgagccgc ccgcgcacgt
     1021 ctcaaccgtg cggctgcatg aaatcctggc cggtttgtct gatgccaagc tggcggcctg
     1081 gccggccagc ttggccgctg aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt
     1141 tgagtaaaac agcttgcgtc atgcggtcgc tgcgtatatg atgcgatgag taaataaaca
     1201 aatacgcaag gggaacgcat gaaggttatc gctgtactta accagaaagg cgggtcaggc
     1261 aagacgacca tcgcaaccca tctagcccgc gccctgcaac tcgccggggc cgatgttctg
     1321 ttagtcgatt ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa
     1381 ccgctaaccg ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc
     1441 cggcgcgact tcgtagtgat cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg
     1501 atcaaggcag ccgacttcgt gctgattccg gtgcagccaa gcccttacga catatgggcc
     1561 accgccgacc tggtggagct ggttaagcag cgcattgagg tcacggatgg aaggctacaa
     1621 gcggcctttg tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag
     1681 gcgctggccg ggtacgagct gcccattctt gagtcccgta tcacgcagcg cgtgagctac
     1741 ccaggcactg ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc
     1801 cgcgaggtcc aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt taatgaggta
     1861 aagagaaaat gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc gcacgcagca
     1921 gcaaggctgc aacgttggcc agcctggcag acacgccagc catgaagcgg gtcaactttc
     1981 agttgccggc ggaggatcac accaagctga agatgtacgc ggtacgccaa ggcaagacca
     2041 ttaccgagct gctatctgaa tacatcgcgc agctaccaga gtaaatgagc aaatgaataa
     2101 atgagtagat gaattttagc ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc
     2161 accgacgccg tggaatgccc catgtgtgga ggaacgggcg gttggccagg cgtaagcggc
     2221 tgggttgtct gccggccctg caatggcact ggaaccccca agcccgagga atcggcgtga
     2281 gcggtcgcaa accatccggc ccggtacaaa tcggcgcggc gctgggtgat gacctggtgg
     2341 agaagttgaa ggccgcgcag gccgcccagc ggcaacgcat cgaggcagaa gcacgccccg
     2401 gtgaatcgtg gcaagcggcc gctgatcgaa tccgcaaaga atcccggcaa ccgccggcag
     2461 ccggtgcgcc gtcgattagg aagccgccca agggcgacga gcaaccagat tttttcgttc
     2521 cgatgctcta tgacgtgggc acccgcgata gtcgcagcat catggacgtg gccgttttcc
     2581 gtctgtcgaa gcgtgaccga cgagctggcg aggtgatccg ctacgagctt ccagacgggc
     2641 acgtagaggt ttccgcaggg ccggccggca tggccagtgt gtgggattac gacctggtac
     2701 tgatggcggt ttcccatcta accgaatcca tgaaccgata ccgggaaggg aagggagaca
     2761 agcccggccg cgtgttccgt ccacacgttg cggacgtact caagttctgc cggcgagccg
     2821 atggcggaaa gcagaaagac gacctggtag aaacctgcat tcggttaaac accacgcacg
     2881 ttgccatgca gcgtacgaag aaggccaaga acggccgcct ggtgacggta tccgagggtg
     2941 aagccttgat tagccgctac aagatcgtaa agagcgaaac cgggcggccg gagtacatcg
     3001 agatcgagct agctgattgg atgtaccgcg agatcacaga aggcaagaac ccggacgtgc
     3061 tgacggttca ccccgattac tttttgatcg atcccggcat cggccgtttt ctctaccgcc
     3121 tggcacgccg cgccgcaggc aaggcagaag ccagatggtt gttcaagacg atctacgaac
     3181 gcagtggcag cgccggagag ttcaagaagt tctgtttcac cgtgcgcaag ctgatcgggt
     3241 caaatgacct gccggagtac gatttgaagg aggaggcggg gcaggctggc ccgatcctag
     3301 tcatgcgcta ccgcaacctg atcgagggcg aagcatccgc cggttcctaa tgtacggagc
     3361 agatgctagg gcaaattgcc ctagcagggg aaaaaggtcg aaaaggtctc tttcctgtgg
     3421 atagcacgta cattgggaac ccaaagccgt acattgggaa ccggaacccg tacattggga
     3481 acccaaagcc gtacattggg aaccggtcac acatgtaagt gactgatata aaagagaaaa
     3541 aaggcgattt ttccgcctaa aactctttaa aacttattaa aactcttaaa acccgcctgg
     3601 cctgtgcata actgtctggc cagcgcacag ccgaagagct gcaaaaagcg cctacccttc
     3661 ggtcgctgcg ctccctacgc cccgccgctt cgcgtcggcc tatcgcggcc gctggccgct
     3721 caaaaatggc tggcctacgg ccaggcaatc taccagggcg cggacaagcc gcgccgtcgc
     3781 cactcgaccg ccggcgccca catcaaggca ccctgcctcg cgcgtttcgg tgatgacggt
     3841 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
     3901 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc
     3961 atgacccagt cacgtagcga tagcggagtg tatactggct taactatgcg gcatcagagc
     4021 agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa
     4081 aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     4141 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     4201 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     4261 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     4321 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     4381 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     4441 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     4501 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     4561 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     4621 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     4681 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
     4741 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     4801 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     4861 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     4921 cacgttaagg gattttggtc atgcatgata tatctcccaa tttgtgtagg gcttattatg
     4981 cacgcttaaa aataataaaa gcagacttga cctgatagtt tggctgtgag caattatgtg
     5041 cttagtgcat ctaatcgctt gagttaacgc cggcgaagcg gcgtcggctt gaacgaattt
     5101 ctagctagag gatcgcacca ataactgcct taaaaaaatt acgccccgcc ctgccactca
     5161 tcgcagtact gttgtaattc attaagcatt ctgccgacat ggaagccatc acaaacggca
     5221 tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc
     5281 attgtgaaaa cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg
     5341 aaactcaccc agggattggc tgagacgaaa aacatattct caataaaccc tttagggaaa
     5401 taggccaggt tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg
     5461 aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt cagtttgctc atggaaaacg
     5521 gtgtaacaag ggtgaacact atcccatatc accagctcac cgtctttcat tgccatacgg
     5581 aactccggat gagcattcat caggcgggca agaatgtgaa taaaggccgg ataaaacttg
     5641 tgcttatttt tctttacggt ctttaaaaag gccgtaatat ccagctgaac ggtctggtta
     5701 taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat
     5761 atatcaacgg tggtatatcc agtgattttt ttctccatga tgtttaactt tgttttaggg
     5821 cgactgccct gctgcgtaac atcgttgctg ctccataaca tcaaacatcg acccacggcg
     5881 taacgcgctt gctgcttgga tgcccgaggc atagactgta ccccaaaaaa acatgtcata
     5941 acaagaagcc atgaaaaccg ccactgcgcc gttaccaccg ctgcgttcgg tcaaggttct
     6001 ggaccagttg cgtgacggca gttacgctac ttgcattaca gcttacgaac cgaacgaggc
     6061 ttatgtccac tgggttcgtg cccgaattga tcacaggcag caacgctctg tcatcgttac
     6121 aatcaacatg ctaccctccg cgagatcatc cgtgtttcaa acccggcagc ttagttgccg
     6181 ttcttccgaa tagcatcggt aacatgagca aagtctgccg ccttacaacg gctctcccgc
     6241 tgacgccgtc ccggactgat gggctgcctg tatcgagtgg tgattttgtg ccgagctgcc
     6301 ggtcggggag ctgttggctg gctggtggca ggatatattg tggtgtaaac aaattgacgc
     6361 ttagacaact taataacaca ttgcggacgt ttttaatgta ctgaattaac gccgaattaa
     6421 ttcgggggat ctggatttta gtactggatt ttggttttag gaattagaaa ttttattgat
     6481 agaagtattt tacaaataca aatacatact aagggtttct tatatgctca acacatgagc
     6541 gaaaccctat aggaacccta attcccttat ctgggaacta ctcacacatt attatggaga
     6601 aactcgagct tgtcgatcga cagatccggt cggcatctac tctatttctt tgccctcgga
     6661 cgagtgctgg ggcgtcggtt tccactatcg gcgagtactt ctacacagcc atcggtccag
     6721 acggccgcgc ttctgcgggc gatttgtgta cgcccgacag tcccggctcc ggatcggacg
     6781 attgcgtcgc atcgaccctg cgcccaagct gcatcatcga aattgccgtc aaccaagctc
     6841 tgatagagtt ggtcaagacc aatgcggagc atatacgccc ggagtcgtgg cgatcctgca
     6901 agctccggat gcctccgctc gaagtagcgc gtctgctgct ccatacaagc caaccacggc
     6961 ctccagaaga agatgttggc gacctcgtat tgggaatccc cgaacatcgc ctcgctccag
     7021 tcaatgaccg ctgttatgcg gccattgtcc gtcaggacat tgttggagcc gaaatccgcg
     7081 tgcacgaggt gccggacttc ggggcagtcc tcggcccaaa gcatcagctc atcgagagcc
     7141 tgcgcgacgg acgcactgac ggtgtcgtcc atcacagttt gccagtgata cacatgggga
     7201 tcagcaatcg cgcatatgaa atcacgccat gtagtgtatt gaccgattcc ttgcggtccg
     7261 aatgggccga acccgctcgt ctggctaaga tcggccgcag cgatcgcatc catagcctcc
     7321 gcgaccggtt gtagaacagc gggcagttcg gtttcaggca ggtcttgcaa cgtgacaccc
     7381 tgtgcacggc gggagatgca ataggtcagg ctctcgctaa actccccaat gtcaagcact
     7441 tccggaatcg ggagcgcggc cgatgcaaag tgccgataaa cataacgatc tttgtagaaa
     7501 ccatcggcgc agctatttac ccgcaggaca tatccacgcc ctcctacatc gaagctgaaa
     7561 gcacgagatt cttcgccctc cgagagctgc atcaggtcgg agacgctgtc gaacttttcg
     7621 atcagaaact tctcgacaga cgtcgcggtg agttcaggct ttttcatatc tcattgcccc
     7681 ccgggatctg cgaaagctcg agagagatag atttgtagag agagactggt gatttcagcg
     7741 tgtcctctcc aaatgaaatg aacttcctta tatagaggaa ggtcttgcga aggatagtgg
     7801 gattgtgcgt catcccttac gtcagtggag atatcacatc aatccacttg ctttgaagac
     7861 gtggttggaa cgtcttcttt ttccacgatg ctcctcgtgg gtgggggtcc atctttggga
     7921 ccactgtcgg cagaggcatc ttgaacgata gcctttcctt tatcgcaatg atggcatttg
     7981 taggtgccac cttccttttc tactgtcctt ttgatgaagt gacagatagc tgggcaatgg
     8041 aatccgagga ggtttcccga tattaccctt tgttgaaaag tctcaatagc cctttggtct
     8101 tctgagactg tatctttgat attcttggag tagacgagag tgtcgtgctc caccatgtta
     8161 tcacatcaat ccacttgctt tgaagacgtg gttggaacgt cttctttttc cacgatgctc
     8221 ctcgtgggtg ggggtccatc tttgggacca ctgtcggcag aggcatcttg aacgatagcc
     8281 tttcctttat cgcaatgatg gcatttgtag gtgccacctt ccttttctac tgtccttttg
     8341 atgaagtgac agatagctgg gcaatggaat ccgaggaggt ttcccgatat taccctttgt
     8401 tgaaaagtct caatagccct ttggtcttct gagactgtat ctttgatatt cttggagtag
     8461 acgagagtgt cgtgctccac catgttggca agctgctcta gccaatacgc aaaccgcctc
     8521 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     8581 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     8641 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     8701 caggaaacag ctatgaccat gattacgaat tcgagctcgg tacccgggga tcctctagag
     8761 tcgacctgca ggcatgcaag cttggcactg gccgtcgttt tacaacgtcg tgactgggaa
     8821 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     8881 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     8941 tgctagagca gcttgagctt ggatcagatt gtcgtttccc gccttcagtt taaactatca
     9001 gtgtttgaca ggatatattg gcgggtaaac ctaagagaaa agagcgttta ttagaataac
     9061 ggatatttaa aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt g
//
