LOCUS       .                       6813 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             2714..3787
                     /codon_start=1
                     /note=""
                     /note="color: #993366"
                     /product="replication protein from Pseudomonas plasmid
                     pVS1"
                     /protein_id=""
                     /transl_table=1
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS*"
                     /label="pVS1 RepA"
     misc_feature    6738..6773
                     /note="multiple cloning site from pUC8"
                     /note="color: #99ccff"
                     /label="MCS"
     CDS             6..23
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /transl_table=1
                     /translation="HHHHHH"
                     /label="6xHis"
                     /note="color: #cc99b2"
     misc_feature    6611..6635
                     /note="left border repeat from nopaline C58 T-DNA"
                     /note="color: #ffe4c4"
                     /label="LB T-DNA repeat"
     misc_feature    332..356
                     /note="right border repeat from nopaline C58 T-DNA"
                     /note="color: #ffe4c4"
                     /label="RB T-DNA repeat"
     misc_feature    4391..4531
                     /note="basis of mobility region from pBR322"
                     /note="color: #a6acb3"
                     /label="bom"
     terminator      58..310
                     /note="nopaline synthase terminator and poly(A) signal"
                     /note="color: #ffffff"
                     /label="NOS terminator"
     rep_origin      complement(4717..5305)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /label="ori"
     CDS             1656..2285
                     /codon_start=1
                     /product="stability protein from Pseudomonas plasmid pVS1"
                     /transl_table=1
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI*"
                     /label="pVS1 StaA"
                     /note="color: #993366"
     CDS             complement(5392..6186)
                     /codon_start=1
                     /gene="aphA-3"
                     /note="confers resistance to kanamycin"
                     /note="color: #ccffcc"
                     /product="aminoglycoside phosphotransferase"
                     /protein_id=""
                     /transl_table=1
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF*"
                     /label="KanR"
     rep_origin      3853..4047
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /note="color: #ffff00"
                     /label="pVS1 oriV"
ORIGIN
        1 ctagccacca ccaccaccac cacgtgtgaa ttacaggtga ccagctcgaa tttccccgat
       61 cgttcaaaca tttggcaata aagtttctta agattgaatc ctgttgccgg tcttgcgatg
      121 attatcatat aatttctgtt gaattacgtt aagcatgtaa taattaacat gtaatgcatg
      181 acgttattta tgagatgggt ttttatgatt agagtcccgc aattatacat ttaatacgcg
      241 atagaaaaca aaatatagcg cgcaaactag gataaattat cgcgcgcggt gtcatctatg
      301 ttactagatc gggaattaaa ctatcagtgt ttgacaggat atattggcgg gtaaacctaa
      361 gagaaaagag cgtttattag aataacggat atttaaaagg gcgtgaaaag gtttatccgt
      421 tcgtccattt gtatgtgcat gccaaccaca gggttcccct cgggatcaaa gtactttgat
      481 ccaacccctc cgctgctata gtgcagtcgg cttctgacgt tcagtgcagc cgtcttctga
      541 aaacgacatg tcgcacaagt cctaagttac gcgacaggct gccgccctgc ccttttcctg
      601 gcgttttctt gtcgcgtgtt ttagtcgcat aaagtagaat acttgcgact agaaccggag
      661 acattacgcc atgaacaaga gcgccgccgc tggcctgctg ggctatgccc gcgtcagcac
      721 cgacgaccag gacttgacca accaacgggc cgaactgcac gcggccggct gcaccaagct
      781 gttttccgag aagatcaccg gcaccaggcg cgaccgcccg gagctggcca ggatgcttga
      841 ccacctacgc cctggcgacg ttgtgacagt gaccaggcta gaccgcctgg cccgcagcac
      901 ccgcgaccta ctggacattg ccgagcgcat ccaggaggcc ggcgcgggcc tgcgtagcct
      961 ggcagagccg tgggccgaca ccaccacgcc ggccggccgc atggtgttga ccgtgttcgc
     1021 cggcattgcc gagttcgagc gttccctaat catcgaccgc acccggagcg ggcgcgaggc
     1081 cgccaaggcc cgaggcgtga agtttggccc ccgccctacc ctcaccccgg cacagatcgc
     1141 gcacgcccgc gagctgatcg accaggaagg ccgcaccgtg aaagaggcgg ctgcactgct
     1201 tggcgtgcat cgctcgaccc tgtaccgcgc acttgagcgc agcgaggaag tgacgcccac
     1261 cgaggccagg cggcgcggtg ccttccgtga ggacgcattg accgaggccg acgccctggc
     1321 ggccgccgag aatgaacgcc aagaggaaca agcatgaaac cgcaccagga cggccaggac
     1381 gaaccgtttt tcattaccga agagatcgag gcggagatga tcgcggccgg gtacgtgttc
     1441 gagccgcccg cgcacgtctc aaccgtgcgg ctgcatgaaa tcctggccgg tttgtctgat
     1501 gccaagctgg cggcctggcc ggccagcttg gccgctgaag aaaccgagcg ccgccgtcta
     1561 aaaaggtgat gtgtatttga gtaaaacagc ttgcgtcatg cggtcgctgc gtatatgatg
     1621 cgatgagtaa ataaacaaat acgcaagggg aacgcatgaa ggttatcgct gtacttaacc
     1681 agaaaggcgg gtcaggcaag acgaccatcg caacccatct agcccgcgcc ctgcaactcg
     1741 ccggggccga tgttctgtta gtcgattccg atccccaggg cagtgcccgc gattgggcgg
     1801 ccgtgcggga agatcaaccg ctaaccgttg tcggcatcga ccgcccgacg attgaccgcg
     1861 acgtgaaggc catcggccgg cgcgacttcg tagtgatcga cggagcgccc caggcggcgg
     1921 acttggctgt gtccgcgatc aaggcagccg acttcgtgct gattccggtg cagccaagcc
     1981 cttacgacat atgggccacc gccgacctgg tggagctggt taagcagcgc attgaggtca
     2041 cggatggaag gctacaagcg gcctttgtcg tgtcgcgggc gatcaaaggc acgcgcatcg
     2101 gcggtgaggt tgccgaggcg ctggccgggt acgagctgcc cattcttgag tcccgtatca
     2161 cgcagcgcgt gagctaccca ggcactgccg ccgccggcac aaccgttctt gaatcagaac
     2221 ccgagggcga cgctgcccgc gaggtccagg cgctggccgc tgaaattaaa tcaaaactca
     2281 tttgagttaa tgaggtaaag agaaaatgag caaaagcaca aacacgctaa gtgccggccg
     2341 tccgagcgca cgcagcagca aggctgcaac gttggccagc ctggcagaca cgccagccat
     2401 gaagcgggtc aactttcagt tgccggcgga ggatcacacc aagctgaaga tgtacgcggt
     2461 acgccaaggc aagaccatta ccgagctgct atctgaatac atcgcgcagc taccagagta
     2521 aatgagcaaa tgaataaatg agtagatgaa ttttagcggc taaaggaggc ggcatggaaa
     2581 atcaagaaca accaggcacc gacgccgtgg aatgccccat gtgtggagga acgggcggtt
     2641 ggccaggcgt aagcggctgg gttgtctgcc ggccctgcaa tggcactgga acccccaagc
     2701 ccgaggaatc ggcgtgagcg gtcgcaaacc atccggcccg gtacaaatcg gcgcggcgct
     2761 gggtgatgac ctggtggaga agttgaaggc cgcgcaggcc gcccagcggc aacgcatcga
     2821 ggcagaagca cgccccggtg aatcgtggca agcggccgct gatcgaatcc gcaaagaatc
     2881 ccggcaaccg ccggcagccg gtgcgccgtc gattaggaag ccgcccaagg gcgacgagca
     2941 accagatttt ttcgttccga tgctctatga cgtgggcacc cgcgatagtc gcagcatcat
     3001 ggacgtggcc gttttccgtc tgtcgaagcg tgaccgacga gctggcgagg tgatccgcta
     3061 cgagcttcca gacgggcacg tagaggtttc cgcagggccg gccggcatgg ccagtgtgtg
     3121 ggattacgac ctggtactga tggcggtttc ccatctaacc gaatccatga accgataccg
     3181 ggaagggaag ggagacaagc ccggccgcgt gttccgtcca cacgttgcgg acgtactcaa
     3241 gttctgccgg cgagccgatg gcggaaagca gaaagacgac ctggtagaaa cctgcattcg
     3301 gttaaacacc acgcacgttg ccatgcagcg tacgaagaag gccaagaacg gccgcctggt
     3361 gacggtatcc gagggtgaag ccttgattag ccgctacaag atcgtaaaga gcgaaaccgg
     3421 gcggccggag tacatcgaga tcgagctagc tgattggatg taccgcgaga tcacagaagg
     3481 caagaacccg gacgtgctga cggttcaccc cgattacttt ttgatcgatc ccggcatcgg
     3541 ccgttttctc taccgcctgg cacgccgcgc cgcaggcaag gcagaagcca gatggttgtt
     3601 caagacgatc tacgaacgca gtggcagcgc cggagagttc aagaagttct gtttcaccgt
     3661 gcgcaagctg atcgggtcaa atgacctgcc ggagtacgat ttgaaggagg aggcggggca
     3721 ggctggcccg atcctagtca tgcgctaccg caacctgatc gagggcgaag catccgccgg
     3781 ttcctaatgt acggagcaga tgctagggca aattgcccta gcaggggaaa aaggtcgaaa
     3841 aggtctcttt cctgtggata gcacgtacat tgggaaccca aagccgtaca ttgggaaccg
     3901 gaacccgtac attgggaacc caaagccgta cattgggaac cggtcacaca tgtaagtgac
     3961 tgatataaaa gagaaaaaag gcgatttttc cgcctaaaac tctttaaaac ttattaaaac
     4021 tcttaaaacc cgcctggcct gtgcataact gtctggccag cgcacagccg aagagctgca
     4081 aaaagcgcct acccttcggt cgctgcgctc cctacgcccc gccgcttcgc gtcggcctat
     4141 cgcggccgct ggccgctcaa aaatggctgg cctacggcca ggcaatctac cagggcgcgg
     4201 acaagccgcg ccgtcgccac tcgaccgccg gcgcccacat caaggcaccc tgcctcgcgc
     4261 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     4321 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     4381 ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat actggcttaa
     4441 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca
     4501 cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc
     4561 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     4621 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     4681 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     4741 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     4801 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     4861 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     4921 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     4981 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     5041 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     5101 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     5161 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     5221 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     5281 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     5341 tcagtggaac gaaaactcac gttaagggat tttggtcatg cattctaggt actaaaacaa
     5401 ttcatccagt aaaatataat attttatttt ctcccaatca ggcttgatcc ccagtaagtc
     5461 aaaaaatagc tcgacatact gttcttcccc gatatcctcc ctgatcgacc ggacgcagaa
     5521 ggcaatgtca taccacttgt ccgccctgcc gcttctccca agatcaataa agccacttac
     5581 tttgccatct ttcacaaaga tgttgctgtc tcccaggtcg ccgtgggaaa agacaagttc
     5641 ctcttcgggc ttttccgtct ttaaaaaatc atacagctcg cgcggatctt taaatggagt
     5701 gtcttcttcc cagttttcgc aatccacatc ggccagatcg ttattcagta agtaatccaa
     5761 ttcggctaag cggctgtcta agctattcgt atagggacaa tccgatatgt cgatggagtg
     5821 aaagagcctg atgcactccg catacagctc gataatcttt tcagggcttt gttcatcttc
     5881 atactcttcc gagcaaagga cgccatcggc ctcactcatg agcagattgc tccagccatc
     5941 atgccgttca aagtgcagga cctttggaac aggcagcttt ccttccagcc atagcatcat
     6001 gtccttttcc cgttccacat cataggtggt ccctttatac cggctgtccg tcatttttaa
     6061 atataggttt tcattttctc ccaccagctt atatacctta gcaggagaca ttccttccgt
     6121 atcttttacg cagcggtatt tttcgatcag ttttttcaat tccggtgata ttctcatttt
     6181 agccatttat tatttccttc ctcttttcta cagtatttaa agatacccca agaagctaat
     6241 tataacaaga cgaactccaa ttcactgttc cttgcattct aaaaccttaa ataccagaaa
     6301 acagcttttt caaagttgtt ttcaaagttg gcgtataaca tagtatcgac ggagccgatt
     6361 ttgaaaccgc ggtgatcaca ggcagcaacg ctctgtcatc gttacaatca acatgctacc
     6421 ctccgcgaga tcatccgtgt ttcaaacccg gcagcttagt tgccgttctt ccgaatagca
     6481 tcggtaacat gagcaaagtc tgccgcctta caacggctct cccgctgacg ccgtcccgga
     6541 ctgatgggct gcctgtatcg agtggtgatt ttgtgccgag ctgccggtcg gggagctgtt
     6601 ggctggctgg tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata
     6661 acacattgcg gacgttttta atgtactgaa ttaacgccga attaattcct aggccaccat
     6721 gttgggcccg gcgcgccgaa ttcccgggga tccgtcgacc tgcagccaag ctttaagagg
     6781 agtccaccat ggtagatctg actagtgtta acg
//
