LOCUS       .                      11662 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     rep_origin      5682..10908
                     /note="replication origin from Aspergillus nidulans
                     (Aleksenko and Clutterbuck, 1996)"
                     /note="color: #a6acb3"
                     /label="AMA1"
     CDS             complement(820..1680)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ccffcc"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      complement(61..649)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /label="ori"
     promoter        complement(1681..1785)
                     /gene="bla"
                     /label="AmpR promoter"
                     /note="color: #ffffff"
     CDS             2859..4234
                     /codon_start=1
                     /product="aureobasidin A resistance protein from
                     Aspergillus nidulans (Kuroda et al., 1999)"
                     /transl_table=1
                     /translation="MNQTLPTWKDRTQNQFGKLQIQVPWRSIQLLVPHRMRRKLRSKLR
                     SRASPTSSIASLQTSLSPADTLRSLQSHRWTVYDFQYLLLLIVGIFSLTVIESPGPLGK
                     TAIFSMLLFSLLIPMTRQFFLPFLPIAGWLLFFYACQ,,FIPSDWRPAIWVRVLPALEN
                     ILYGANISNILSAHQNVVLDVLAWLPYGICHYGAPFVCSLIMFIFGPPGTVPLFARTFG
                     YISMTAVTIQLFFPCSPPWYENRYGLAPADYSIQGDPAGLARIDKLFGIDLYTSVFHQS
                     PVVFGAFPSLHAADSTLAALFMSHVFPRMKPVFVTYTLWMWWATMYLSHHYAVDLVAGG
                     LLAAIAFYFAKTRFLPRVQLDKTFRWDYDYVEFGESALEYGYGAAGYDGDFNLDSDEWT
                     VGSSSSVSSGSLSPVDDHYSWETEALTSPHTDIESGRHTFSP*"
                     /label="aurA-R"
                     /note="color: #ccffcc"
     misc_feature    10917..10945
                     /note="multiple cloning site"
                     /note="color: #99ccff"
                     /label="MCS"
ORIGIN
        1 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
       61 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
      121 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      181 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      241 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      301 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      361 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      421 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      481 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
      541 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      601 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
      661 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
      721 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
      781 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
      841 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
      901 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
      961 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     1021 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     1081 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     1141 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     1201 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     1261 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     1321 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     1381 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     1441 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     1501 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     1561 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     1621 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     1681 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     1741 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     1801 agtgccacct gatggtcaga tggatctgcc gtctgttttg atttgaattt tccctgctca
     1861 ttctgattct gtgagaggct gcattcatta tcacatctca tacccggcgc ctgcgacttc
     1921 ggtcacctct gcggtctggc ggttaccggg gtccgtctga gactcgtcag tcagccattc
     1981 gagtatgcga actctgactt tgctcaccta agagtttgca cgagatgccg aaatcctcct
     2041 cgagtagagt ttgcaaggct tgaaccttgg tccttgaagc ccgaaagtgg ctcagtagtg
     2101 ggatcgatag tctggttgtt gaagattttc tcctccacct tacctatggc cgctggcctt
     2161 ctccaccttt caggctttca ggcaccctcg gctcggattc tgtatcgtcc ggtaccgaag
     2221 ctagtcctag ctagtcaaag ctagtccaag ctagtctcgt caaggtttgg cgcagcgcgg
     2281 ttccgtgtaa agtacaaatt tgaaatacga atacgcagta ctcgcagccg gcacttccgc
     2341 tcagcccagg ctcagaggct aagggtgttg gcgcttcctc atcatcttct tctcgtcgac
     2401 cttttcctct ttctctccct atcggtgctt ctctccaacc tcattctcag tcgttcgccc
     2461 atcaggttta tactccggct ccgtggccat ctgcctccct cacgacctcc tcgttccagg
     2521 ttttcctctc gactgctgcg cccttgcact tcgccttgca tcagtgaaac cccctgcaac
     2581 gtgacggctc aaagacatcc tcgtttggcc gctggagacc ggagcgtgcg cttcgtttcg
     2641 tcttcttcga accgatctca atttccccgc tcgggttgac gccgtcagca ccctgctcgt
     2701 tgcctaacgg cttgttattc aagacccctt ttctgccgct tccgcgaccg atttattcgt
     2761 cgccttccaa ctcttgtaca atcgggggga aagaaagcag acggagttcg atctggagga
     2821 attatagctg agtcttgccc gcaagactcg ccgcaaccat gaatcaaaca cttcccacgt
     2881 ggaaggaccg cacgcagaac cagtttggaa agcttcagat ccaggttcca tggcggtcca
     2941 tccaactgct cgtcccgcat cgcatgcggc ggaagttaag gtccaaattg cgcagtagag
     3001 cgtctcctac ctcgtcaata gcctctttac agacgtcgtt atcgcctgca gacacactac
     3061 gatcgctcca aagccaccga tggacggttt acgacttcca atatctgctt ctgttgatcg
     3121 tgggcatctt ctctttgacc gttatcgagt cgcccgggcc tttgggcaaa acggccattt
     3181 tctccatgct cctattctct ctcctgatcc ctatgacccg ccagttcttc ctcccgtttc
     3241 tgccgattgc cggatggctt ctgtttttct acgcctgcca gtgagttaaa aacaacccgc
     3301 taccagaccc cgtgcagcag ttactcacat atgcaggttc atcccaagcg attggcgccc
     3361 tgcgatttgg gttcgtgtct tgcctgcact ggagaatatt ctctacggcg caaacatcag
     3421 caacatccta tccgctcacc agaacgttgt gcttgacgtg ctggcgtggc taccctacgg
     3481 tatctgccac tatggcgctc cgtttgtgtg ctcgttgatc atgttcatct tcggtccgcc
     3541 cggcactgtt ccccttttcg cgcgcacttt cggctatatc agtatgactg cggttactat
     3601 tcagctgttt ttcccttgct ctccaccttg gtatgagaat cgctatggtc tagctccggc
     3661 agactactcc atccaaggtg atcccgcagg gcttgcccgc attgacaagc ttttcggcat
     3721 cgacctttac acgtctgttt tccatcagtc gcctgttgtg ttcggcgctt ttccgtcgct
     3781 gcatgctgcc gactcaaccc tggccgcact tttcatgagt catgttttcc cccgcatgaa
     3841 gcccgtcttc gtgacctata ctctatggat gtggtgggca acaatgtacc tctcacatca
     3901 ctatgcggtc gatttggttg cgggtggtct cctggccgcc attgctttct acttcgccaa
     3961 gacccgattc cttccccgtg tccagctcga caagaccttc cgttgggact acgactatgt
     4021 ggaattcggc gagtctgccc tggagtatgg gtatggtgca gctggctatg atggagactt
     4081 caatctcgac agcgatgaat ggactgttgg ttcttcatcc tccgtctcct caggctcctt
     4141 gagtcccgtt gacgatcatt actcatggga aaccgaggca ctgacctccc cacatactga
     4201 tattgagtcc ggcaggcata ctttcagccc ttgagtagcc acaaaccaaa ctcgatacct
     4261 gcatatagcg atctcgctcc tcctccactg catctattta cgagacggcg ttagaacatt
     4321 tcacgacatt ctggctttat tgcatcgagc acatttcgac acatatatct ttaataccct
     4381 ttcttcggtg tcccagatca tcggttcgac cttaatgtac ctcggtccga atccgcctgg
     4441 gatactgttt ctctttccgc cgcacttcac tgtacattgc ttgacattgc gaaaccgggt
     4501 tgggctcgaa cgtgggatgg gttatcgctc atcgctacac gccgttgctc catcataatg
     4561 ttaatggaca caatggggct acgcatcctg gtgtttagtc ctggaagacc atccgataac
     4621 ccccgtcggt aacactcgct tgtctcgtgt ccacccagac actacttcaa ttctcacttc
     4681 tatcgtccgc tattaccttg acctggtcga acccatcctt attattcgtt tcgactatgc
     4741 tatatattta tttttaccat tcgtgtcgat cgctcatact cttggcgctt gggactggaa
     4801 gcatttatat tggaaaaaat cacggaatgg ggcgcctttt cttcttgcac ttcactcgct
     4861 gtgcatagac ggttttacat ttctgctttg caatgcatca cgaactctgc attagcatat
     4921 agaaagaggg gaaggatgga ccttcttctt gattgctcgc atggtttatc cattcgctca
     4981 aagtggatta cgtccacata ttacccgggg gctatacaca tggctactgt gttgctttct
     5041 gacattcgcc ggacgtgcaa ggttgggagg agagtctgac gctgacgggg cttgttgaag
     5101 gatgttcacg cgtcccgatt tgacccggct tcgactaacc tcagattctc gacttgttgg
     5161 acggtgactt gacttgcttg ctatggtctg acgctctcac acctacctat cacatcctcc
     5221 tcacctcaca aattccgctc atggacacta tcctcttctt ttcgtttccc ttggatagtg
     5281 tgtgtgtgtg tgtggttggg gcaaattatc catagcagca gtattattag ttataatccg
     5341 gtagtgttat gatttatgaa ggcaacttgt atactattgc cactttgtcc atatctcttg
     5401 cttgtaatag aactgacatc gcgacgcttc ggccacgatg catataaaaa ctctagtcaa
     5461 cacgatatta acaagcgaaa ccattacgct gtaaactatt caggatcgcc gcgggcccat
     5521 ctgggacttg actgtactaa atatgtccta aagcaagcag actaaatatt taacgtggga
     5581 tattattcat atacgcatat gtatacatag tcataacaag ccaactaaga aaccattatt
     5641 atcatgacat taacctataa aaataggcgt atcacgaggc ctactgtttt gtgatagcac
     5701 gaagtttttc cacggtatct tgtaaaaata tatatttgtg gcgggcttac ctacatcaaa
     5761 ttaataagag actaattata aactaaacac acaagcaagc tactttaggg taaaagttta
     5821 taaatgcttt tgacgtataa acgttgcttg tatttattat tacaattaaa ggtggataga
     5881 aaacctagag actagttaga aactaatctc aggtttgcgt taaactaaat cagagcccga
     5941 gaggttaaca gaacctagaa ggggactaga tatccgggta gggaaacaaa aaaaaaaaac
     6001 aagacagcca catattaggg agactagtta gaagctagtt ccaggactag gaaaataaaa
     6061 gacaatgata ccacagtcta gttgacaact agatagattc tagattgagg ccaaagtctc
     6121 tgagatccag gttagttgca actaatacta gttagtatct agtctcctat aactctgaag
     6181 ctagaataac ttactactat tatcctcacc actgttcagc tgcgcaaacg gagtgattgc
     6241 aaggtgttca gagactagtt attgactagt cagtgactag caataactaa caaggtatta
     6301 acctaccatg tctgccatca ccctgcactt cctcgggctc agcagccttt tcctcctcat
     6361 tttcatgctc attttccttg tttaagactg tgactagtca aagactagtc cagaaccaca
     6421 aaggagaaat gtcttaccac tttcttcatt gcttgtctct tttgcattat ccatgtctgc
     6481 aactagttag agtctagtta gtgactagtc cgacgaggac ttgcttgtct ccggattgtt
     6541 ggaggaactc tccagggcct caagatccac aacagagcct tctagaagac tggtcaataa
     6601 ctagttggtc tttgtctgag tctgacttac gaggttgcat actcgctccc tttgcctcgt
     6661 caatcgatga gaaaaagcgc caaaactcgc aatatggctt tgaaccacac ggtgctgaga
     6721 ctagttagaa tctagtccca aactagcttg gatagcttac ctttgccctt tgcgttgcga
     6781 caggtcttgc agggtatggt tcctttctca ccagctgatt tagctgcctt gctaccctca
     6841 cggcggatct gccataaaga gtggctagag gttataaatt agcactgatc ctaggtacgg
     6901 ggctgaatgt aacttgcctt tcctttctca tcgcgcggca agacaggctt gctcaaattc
     6961 ctaccagtca caggggtatg cacggcgtac ggaccacttg aactagtcac agattagtta
     7021 gcaactagtc tgcattgaat ggctgtactt acgggccctc gccattgtcc tgatcatttc
     7081 cagcttcacc ctcgttgctg caaagtagtt agtgactagt caaggactag ttgaaatggg
     7141 agaagaaact cacgaattct cgactccctt agtattgtgg tccttggact tggtgctgct
     7201 atatattagc taatacacta gttagactca cagaaactta cgcagctcgc ttgcgcttct
     7261 tggtaggagt cggggttggg agaacagtgc cttcaaacaa gccttcatac catgctactt
     7321 gactagtcag ggactagtca ccaagtaatc tagataggac ttgcctttgg cctccatcag
     7381 ttccttcata gtgggaggac cattgtgcaa tgtaaactcc atgccgtggg agttcttgtc
     7441 cttcaagtgc ttgaccaata tgtttctgtt ggcagaggga acctgtcaac tagttaataa
     7501 ctagtcagaa actatgatag cagtagactc actgtacgct tgaggcatcc cttcactcgg
     7561 cagtagactt catatggatg gatatcaggc acgccattgt cgtcctgtgg actagtcagt
     7621 aactaggctt aaagctagtc gggtcggctt actatcttga aatccggcag cgtaagctcc
     7681 ccgtccttaa ctgcctcgag atagtgacag tactctgggg actttcggag atcgttatcg
     7741 ttatcgcgaa tgctcggcat actaactgtt gactagtctt ggactagtcc cgagcaaaaa
     7801 ggattggagg aggaggagga aggtgagagt gagacaaaga gcgaaataag agcttcaaag
     7861 gctatctcta agcagtatga aggttaagta tctagttctt gactagattt aaagagattt
     7921 cgactagtta tgtacctgga gtttggatat aggaatgtgt tgtggtaacg aaatgtaagg
     7981 gggaggaaag aaaaagtcgt caagaggtaa ctctaagtcg gccattcctt tttgggaggc
     8041 gctaaccata aacggcatgg tcgacttaga gttagctcag ggaatttagg gagttatctg
     8101 cgaccaccga ggaacggcgg aatgccaaag aatcccgatg gagctctagc tggcggttga
     8161 caaccccacc ttttggcgtt tctgcggcgt tgcaggcggg actggatact tcgtagaacc
     8221 agaaaggcaa ggcagaacgc gctcagcaag agtgttggaa gtgatagcat gatgtgcctt
     8281 gttaactagg tcaaaatctg cagtatgctt gatgttatcc aaagtgtgag agaggaaggt
     8341 ccaaacatac acgattggga gagggcctag gtataagagt ttttgagtag aacgcatgtg
     8401 agcccagcca tctcgaggag attaaacacg ggccggcatt tgatggctat gttagtaccc
     8461 caatggaaac ggtgagagtc cagtggtcgc agataactcc ctaaattccc tgagctaact
     8521 ctaagtcgac catgccgttt atggttagcg cctcccaaaa aggaatggcc gacttagagt
     8581 tacctcttga cgactttttc tttcctcccc cttacatttc gttaccacaa cacattccta
     8641 tatccaaact ccaggtacat aactagtcga aatctcttta aatctagtca agaactagat
     8701 acttaacctt catactgctt agagatagcc tttgaagctc ttatttcgct ctttgtctca
     8761 ctctcacctt cctcctcctc ctccaatcct ttttgctcgg gactagtcca agactagtca
     8821 acagttagta tgccgagcat tcgcgataac gataacgatc tccgaaagtc cccagagtac
     8881 tgtcactatc tcgaggcagt taaggacggg gagcttacgc tgccggattt caagatagta
     8941 agccgacccg actagcttta agcctagtta ctgactagtc cacaggacga caatggcgtg
     9001 cctgatatcc atccatatga agtctactgc cgagtgaagg gatgcctcaa gcgtacagtg
     9061 agtctactgc tatcatagtt tctgactagt tattaactag ttgacaggtt ccctctgcca
     9121 acagaaacat attggtcaag cacttgaagg acaagaactc ccacggcatg gagtttacat
     9181 tgcacaatgg tcctcccact atgaaggaac tgatggaggc caaaggcaag tcctatctag
     9241 attacttggt gactagtccc tgactagtca agtagcatgg tatgaaggct tgtttgaagg
     9301 cactgttctc ccaaccccga ctcctaccaa gaagcgcaag cgagctgcgt aagtttctgt
     9361 gagtctaact agtgtattag ctaatatata gcagcaccaa gtccaaggac cacaatacta
     9421 agggagtcga gaattcgtga gtttcttctc ccatttcaac tagtccttga ctagtcacta
     9481 actactttgc agcaacgagg gtgaagctgg aaatgatcag gacaatggcg agggcccgta
     9541 agtacagcca ttcaatgcag actagttgct aactaatctg tgactagttc aagtggtccg
     9601 tacgccgtgc atacccctgt gactggtagg aatttgagca agcctgtctt gccgcgcgat
     9661 gagaaaggaa aggcaagtta cattcagccc cgtacctagg atcagtgcta atttataacc
     9721 tctagccact ctttatggca gatccgccgt gagggtagca aggcagctaa atcagctggt
     9781 gagaaaggaa ccataccctg caagacctgt cgcaacgcaa agggcaaagg taagctatcc
     9841 aagctagttt gggactagat tctaactagt ctcagcaccg tgtggttcaa agccatattg
     9901 cgagttttgg cgctttttct catcgattga cgaggcaaag ggagcgagta tgcaacctcg
     9961 taagtcagac tcagacaaag accaactagt tattgaccag tcttctagaa ggctctgttg
    10021 tggatcttga ggccctggag agttcctcca acaatccgga gacaagcaag tcctcgtcgg
    10081 actagtcact aactagactc taactagttg cagacatgga taatgcaaaa gagacaagca
    10141 atgaagaaag tggtaagaca tttctccttt gtggttctgg actagtcttt gactagtcac
    10201 agtcttaaac aaggaaaatg agcatgaaaa tgaggaggaa aaggctgctg agcccgagga
    10261 agtgcagggt gatggcagac atggtaggtt aataccttgt tagttattgc tagtcactga
    10321 ctagtcaata actagtctct gaacaccttg caatcactcc gtttgcgcag ctgaacagtg
    10381 gtgaggataa tagtagtaag ttattctagc ttcagagtta taggagacta gatactaact
    10441 agtattagtt gcaactaacc tggatctcag agactttggc ctcaatctag aatctatcta
    10501 gttgtcaact agactgtggt atcattgtct tttattttcc tagtcctgga actagcttct
    10561 aactagtctc cctaatatgt ggctgtcttg tttttttttt ttgtttccct acccggatat
    10621 ctagtcccct tctaggttct gttaacctct cgggctctga tttagtttaa cgcaaacctg
    10681 agattagttt ctaactagtc tctaggtttt ctatccacct ttaattgtaa taataaatac
    10741 aagcaacgtt tatacgtcaa aagcatttat aaacttttac cctaaagtag cttgcttgtg
    10801 tgtttagttt ataattagtc tcttattaat ttgatgtagg taagcccgcc acaaatatat
    10861 atttttacaa gataccgtgg aaaaacttcg tgctatcaca aaacagtagc cctagaggat
    10921 cctagcgctt aagatctcct gcaggcctct agatagctcg tgaagggtgg agagtatatg
    10981 atggtactgc tattcaatct ggcattggac agtgagtttg agtttgatgt acagttggag
    11041 tcgttactgc tgtcatcccc ttatactctt cgattgtttt tcgaacccta acgccaagca
    11101 cgctagtcta ttataggaaa ggatcgatcc ggattaatgt gttttcataa cgcggtactg
    11161 tatggtactt ctgtattata tcaccgaagc tcatgtatct tacatgtata tattatacag
    11221 acacaacctt ggttacccca ccatgatgtt tcctgcagat aatctcctga cgatcaatct
    11281 taccacaggg atatgatggc acccaacctg gcgccttcgc aacatcaaat tatttgtgat
    11341 atgatcaagt gcgatccatc acttactaat gcccagatag ctgaagctgc taactgcagc
    11401 acacgcgcaa ttcctaggat tcggtcaaat ctccggctat tcggcagtag caaagcccct
    11461 ccaaataaag gtggacgccc acgaagcatc tcaccaataa tgctggaggc tctttgtgat
    11521 catcttcttg aaaagcctga tctatacctt gacgaaatgg ccatctttct atgggatgag
    11581 ttccaaatat acgcaactac atctagtatc aggcgggctc tgtcttctaa aggttggtcc
    11641 aaaaaggcag ctcggcagaa ag
//
