LOCUS       .                       6687 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             3805..5010
                     /codon_start=1
                     /gene="S. cerevisiae AUR1 with a dominant F158Y mutation"
                     /note="confers fungal resistance to aureobasidin A"
                     /note="color: #ccffcc"
                     /product="aureobasidin A-resistant mutant of S. cerevisiae
                     inositol phosphorylceramide synthase (Aeed et al., 2009)"
                     /transl_table=1
                     /translation="MANPFSRWFLSERPPNCHVADLETSLDPHQTLLKVQKYKPALSDW
                     VHYIFLGSIMLFVFITNPAPWIFKILFYCFLGTLFIIPATSQFFFNALPILTWVALYFT
                     SSYFPDDRRPPITVKVLPAVETILYGDNLSDILATSTNSFLDILAWLPYGLFHYGAPFV
                     VAAILFVFGPPTVLQGYAFAFGYMNLFGVIMQNVFPAAPPWYKILYGLQSANYDMHGSP
                     GGLARIDKLLGINMYTTCFSNSSVIFGAFPSLHSGCATMEALFFCYCFPKLKPLFIAYV
                     CWLWWSTMYLTHHYFVDLMAGSVLSYVIFQYTKYTHLPIVDTSLFCRWSYTSIEKYDIS
                     KSDPLAADSNDIESVPLSNLELDFDLNMTDEPSVSPSLFDGSTSVSRSSATSITSLGVK
                     RA*"
                     /label="AurR"
     CDS             201..1061
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ccffcc"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      1232..1820
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /label="ori"
     rep_origin      6046..6501
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
                     /label="f1 ori"
     promoter        96..200
                     /gene="bla"
                     /label="AmpR promoter"
                     /note="color: #ffffff"
     misc_feature    5766..5810
                     /gene=""
                     /note="multiple cloning site"
                     /note="color: #99ccff"
                     /label="MCS"
     primer_bind     complement(5817..5833)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /note="color: #a020f0"
                     /label="M13 fwd"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     2221 accatgatta cgccaagctt tgtaaagttc ttcttccaga ttgtgcgcaa actcttgcga
     2281 tatcgatata acgtctttcc catcaggaag ctcataaagt tttgcctcaa ttgtttcggg
     2341 aacgatgaat ttgctaaata aggtagagaa cattttttct gcattatacc tcagtttgtg
     2401 ttctttttcg ctttcaaaat ctttcctagt aggcatttcg ttttcggcag tatcagactt
     2461 ggcggcatca atgcttttat tccttttttt tgacccattg gatttcttca cctgtttgga
     2521 tttgatatga attcctttgg cactatcggg gctccttttt cgtttcctag ggctttcctc
     2581 atcaagaaag acatctgcat cattgtcatc atggtcattt acgggcttgt aaacatcatc
     2641 atggtaatct tcatcctctg aaacctctgc ctcctttgac gtttccaaat gtgcatacaa
     2701 tgatgggtcg cacaattcac agtagtactt agaatcttca ctcattagcc catctatggt
     2761 gtctttacca tcagtcatac atttaatatg ctgccaagtg tcacagccat cgcactgtac
     2821 catgtcgcca tgcgaatatt ctgcagcatc gtagttttca ttgttcgccc cacacagaca
     2881 tctaacatag ccctcatccg cttcgtctac ttcgtctgtt cttacatttt cagtatcttt
     2941 ccttggctcc tgagaagaat cagacttctt attcttctct atggctgaat caactttctt
     3001 tttagttctt tttttctttg gtgcttcagt ctcctcctgt aacaaatact caatatattt
     3061 attctgaccc ttattagatc ttgaagaagt acggacagac ataataaatt taaactaaat
     3121 aaaagctaaa agtaaaagag aacctaggat aatcgatagc tccctgtgta tagaacaagt
     3181 caaaatacta agatctcgtc aacctatcct ctttcattgc aagccttttg gccaaactgc
     3241 agtctgggca tccctttgca tgtcttgtga aatagtatcc atctctcccc agctcccaga
     3301 aatgatatga aaagttattc gtgaagagtg atcctaaaac tgaaaaaagg aaaaaacaaa
     3361 aatgagctaa aaaaaccaca acaaaagaat atcaacttca ttgcttggcg ggtcatcgct
     3421 tttttatttc tttctgtcaa agaataataa agtgcccatc agtgttcata tttgttacaa
     3481 agtggttttc tgatttggta ctactgcaga ggcgtatttt ttgcttcagt taccatagcg
     3541 taagaacact agcgactttt gttcgtgaac caacagagta ggatttctac tgctacatct
     3601 cttaggtagt tggttagtcc gatcgctcac ttttggttgt tgttaagtac ttcataagtt
     3661 tatccttttc ctttttcaca ctgagctact ttgggtatag cttttggccc aaggatcttt
     3721 gaattttctc caaaagtact ttattttata tcctacaggt tgcggttttc atattttaaa
     3781 aagcttttaa tcattccttt gcgtatggca aacccttttt cgagatggtt tctatcagag
     3841 agacctccaa actgccatgt agccgattta gaaacaagtt tagatcccca tcaaacgttg
     3901 ttgaaggtgc aaaaatacaa acccgcttta agcgactggg tgcattacat cttcttggga
     3961 tccatcatgc tgtttgtgtt cattactaat cccgcacctt ggatcttcaa gatccttttt
     4021 tattgtttct tgggcacttt attcatcatt ccagctacgt cacagttttt cttcaatgcc
     4081 ttgcccatcc taacatgggt ggcgctgtat ttcacttcat cgtactttcc agatgaccgc
     4141 aggcctccta ttactgtcaa agtgttacca gcggtggaaa caattttata cggcgacaat
     4201 ttaagtgata ttcttgcaac atcgacgaat tcctttttgg acattttagc atggttaccg
     4261 tacggactat ttcattatgg ggccccattt gtcgttgctg ccatcttatt cgtatttggt
     4321 ccaccaactg ttttgcaagg ttatgctttt gcatttggtt atatgaacct gtttggtgtt
     4381 atcatgcaaa atgtctttcc agccgctccc ccatggtata aaattctcta tggattgcaa
     4441 tcagccaact atgatatgca tggctcgcct ggtggattag ctagaattga taagctactc
     4501 ggtattaata tgtatactac atgtttttca aattcctccg tcattttcgg tgcttttcct
     4561 tcactgcatt ccgggtgtgc tactatggaa gccctgtttt tctgttattg ttttccaaaa
     4621 ttgaagccct tgtttattgc ttatgtttgc tggttatggt ggtcaactat gtatctgaca
     4681 caccattatt ttgtagacct tatggcaggt tctgtgctgt catacgttat tttccagtac
     4741 acaaagtaca cacatttacc aattgtagat acatctcttt tttgcagatg gtcatacact
     4801 tcaattgaga aatacgatat atcaaagagt gatccattgg ctgcagattc aaacgatatc
     4861 gaaagtgtcc ctttgtccaa cttggaactt gactttgatc ttaatatgac tgatgaaccc
     4921 agtgtaagcc cttcgttatt tgatggatct acttctgttt ctcgttcgtc cgccacgtct
     4981 ataacgtcac taggtgtaaa gagggcttaa tgagtatttt atctgcaatt acggatacgg
     5041 ttggtcttat gtagatacat ataaatatat atctttttct ttctttttct tagtcaggat
     5101 tgtcgtttag cataatatac atgtagttta tttaatcaca taccactgat tatctttaga
     5161 attttataaa tttttgaaat aaatgggtgg cttttaatgg tgtctatgtt aagtgaggct
     5221 tttagaatgc tcttcctgct ttgtttatta tatgtgtatg aaagatatgt atgtatttac
     5281 atgtgtttgt agcgtcccca gtcaaaacct gtgcgctata cctaaatgga ttgataatct
     5341 tcattcacta attctaaaat agacttcttc cccaaagaac ggtgtaacga tgaggctcta
     5401 tccagctgct tatctaaatc aactttaacg atggatgatc ttatgacacg gggatctttc
     5461 tttaaagttc ttagaatttc agactgtacc gcagctgatg aatcaaacag cattaaaaag
     5521 tgatatgctc gaaaatgttt ttcctggtct ttcttcatta ttttaggaag ataccttatg
     5581 cccatgggta caatgtccct caccacacct ctgttttgaa taatcagttt cccgattgtg
     5641 gaagacaatt cttttgcttc caactttggc gcattggagt tggttatgcg aacaagtccg
     5701 atcagctcat aaagcatctt agtgaaaagg gtggttttgc gttattcttt cctctgttga
     5761 agcttgcatg cctgcaggtc gactctagag gatccccggg taccgagctc gaattcactg
     5821 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     5881 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     5941 tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt tctccttacg
     6001 catctgtgcg gtatttcaca ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg
     6061 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     6121 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     6181 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     6241 tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg ccctgataga
     6301 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     6361 ctggaacaac actcaaccct atctcgggct attcttttga tttataaggg attttgccga
     6421 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     6481 aaatattaac gtttacaatt ttatggtgca ctctcagtac aatctgctct gatgccgcat
     6541 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     6601 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     6661 tttcaccgtc atcaccgaaa cgcgcga
//
