LOCUS       .                       3684 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        complement(1322..1340)
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff"
                     /label="T7 promoter"
     promoter        complement(3218..3322)
                     /gene="bla"
                     /label="AmpR promoter"
                     /note="color: #ffffff"
     terminator      1017..1214
                     /note="Ashbya gossypii TEF terminator"
                     /note="color: #ffffff"
                     /label="TEF terminator"
     promoter        115..458
                     /note="Ashbya gossypii TEF promoter"
                     /note="color: #ffffff"
                     /label="TEF promoter"
     CDS             511..966
                     /codon_start=1
                     /gene="Streptomyces viridochromogenes pat1"
                     /note="confers resistance to bialaphos or phosphinothricin"
                     /note="color: #ccffcc"
                     /product="phosphinothricin acetyltransferase"
                     /transl_table=1
                     /translation="MAAVCDIVNHYIETSTVNFRTEPQTPQEWIDDLERLQDRYPWLVA
                     EVEGVVAGIAYAGPWKARNAYDWTVESTVYVSHRHQRLGLGSTLYTHLLKSMETQGFKS
                     VVAVIGLPNDPSVRLHEALGYTARGTLRAAGYKHGGWHDVGFWQRDF*"
                     /label="BlpR"
     rep_origin      complement(1598..2186)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /label="ori"
     CDS             complement(2357..3217)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ccffcc"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     gene            115..1214
                     /product="yeast selectable marker conferring bialaphos
                     resistance (Goldstein and McCusker, 1999)"
                     /label="patMX4"
                     /note="color: #ff7f50"
ORIGIN
        1 gaacgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acggatcccc gggttaatta
       61 aggcgcgcca gatctgttta gcttgccttg tccccgccgg gtcacccggc cagcgacatg
      121 gaggcccaga ataccctcct tgacagtctt gacgtgcgca gctcaggggc atgatgtgac
      181 tgtcgcccgt acatttagcc catacatccc catgtataat catttgcatc catacatttt
      241 gatggccgca cggcgcgaag caaaaattac ggctcctcgc tgcagacctg cgagcaggga
      301 aacgctcccc tcacagacgc gttgaattgt ccccacgccg cgcccctgta gagaaatata
      361 aaaggttagg atttgccact gaggttcttc tttcatatac ttccttttaa aatcttgcta
      421 ggatacagtt ctcacatcac atccgaacat aaacaaccgg gtagcccaga acgacgcccg
      481 gtcgagatcc gtcccgccac cgccgccgac atggcggcgg tctgcgacat cgtcaatcac
      541 tacatcgaga cgagcacggt caacttccgt acggagccgc agactccgca ggagtggatc
      601 gacgacctgg agcgcctcca ggaccgctac ccctggctcg tcgccgaggt ggagggcgtc
      661 gtcgccggca tcgcctacgc cggcccctgg aaggcccgca acgcctacga ctggaccgtc
      721 gagtcgacgg tgtacgtctc ccaccggcac cagcggctcg gactgggctc caccctctac
      781 acccacctgc tgaagtccat ggagacccag ggcttcaaga gcgtggtcgc cgtcatcgga
      841 ctgcccaacg acccgagcgt gcgcctgcac gaggcgctcg gatacaccgc gcgcgggacg
      901 ctgcgggcag ccggctacaa gcacgggggc tggcacgacg tggggttctg gcagcgcgac
      961 ttctagctgc cggccccgcc ccgccccgtc cggcccgtca cacagatcta atcaatactg
     1021 acaataaaaa gattcttgtt ttcaagaact tgtcatttgt atagtttttt tatattgtag
     1081 ttgttctatt ttaatcaaat gttagcgtga tttatatttt ttttcgcctc gacatcatct
     1141 gcccagatgc gaagttaagt gcgcagaaag taatatcatg cgtcaatcgt atgtgaatgc
     1201 tggtcgctat actgctgtcg attcgatact aacgccgcca tccagtgtcg aaaacgagct
     1261 cgaattcatc gatgatatca gatccactag tggcctatgc ggccgcggat ctgccggtct
     1321 ccctatagtg agtcgtatta atttcgataa gccaggttaa cctgcattaa tgaatcggcc
     1381 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     1441 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     1501 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     1561 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     1621 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     1681 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     1741 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct caatgctcac
     1801 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     1861 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     1921 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     1981 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     2041 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     2101 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     2161 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     2221 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     2281 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     2341 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     2401 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     2461 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     2521 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     2581 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     2641 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     2701 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     2761 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     2821 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     2881 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     2941 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     3001 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     3061 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     3121 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     3181 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     3241 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     3301 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
     3361 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg
     3421 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
     3481 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
     3541 gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc
     3601 atatggacat attgtcgtta gaacgcggct acaattaata cataacctta tgtatcatac
     3661 acatacgatt taggtgacac tata
//
