LOCUS . 7507 bp DNA circular UNK 01-JAN-1980 DEFINITION . ACCESSION VERSION KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers CDS 6351..6374 /codon_start=1 /product="FLAG® epitope tag, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDDDDK" /label="FLAG" /note="color: #cc99b2" CDS 6303..6332 /codon_start=1 /product="Myc (human c-Myc oncogene) epitope tag" /transl_table=1 /translation="EQKLISEEDL" /label="Myc" /note="color: #cc99b2" promoter complement(2117..2221) /gene="bla" /label="AmpR promoter" /note="color: #ffffff" promoter 5941..6144 /note="human cytomegalovirus (CMV) immediate early promoter" /note="color: #ffffff" /label="CMV promoter" enhancer 5637..5940 /note="human cytomegalovirus immediate early enhancer" /note="color: #ffffff" /label="CMV enhancer" rep_origin complement(2503..2958) /direction=LEFT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /note="color: #ffff00" /label="f1 ori" polyA_signal 6421..6897 /note="human growth hormone polyadenylation signal" /note="color: #a6acb3" /label="hGH poly(A) signal" promoter complement(5020..5519) /note="mouse phosphoglycerate kinase 1 promoter" /note="color: #ffffff" /label="PGK promoter" rep_origin complement(497..1085) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /note="color: #ffff00" /label="ori" CDS complement(4381..4980) /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /note="color: #ccffcc" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APHNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" CDS complement(1256..2116) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /note="color: #ccffcc" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" misc_feature 6197..6300 /note="multiple cloning site" /note="color: #99ccff" /label="MCS" misc_feature 6972..7501 /note="right homology arm from the adeno-associated virus integration site (AAVS1) within intron 1 of the human PPP1R12C gene" /note="color: #ffe4c4" /label="HA-R" misc_feature 3278..3779 /note="left homology arm from the adeno-associated virus integration site (AAVS1) within intron 1 of the human PPP1R12C gene" /note="color: #ffe4c4" /label="HA-L" ORIGIN 1 atcactctcg ccggttggac tttagatcag aagggatctt gctgccgccc gaaagaggaa 61 gggctggaag aggaaggagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt 121 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg 181 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt 241 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt 301 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 361 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg 421 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg 481 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 541 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 601 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 661 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg 721 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 781 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 841 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 901 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc 961 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 1021 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 1081 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 1141 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt 1201 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc 1261 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg 1321 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg 1381 ctgcaatgat accgcgagaa ccacgctcac cggctccaga tttatcagca ataaaccagc 1441 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta 1501 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg 1561 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct 1621 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta 1681 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg 1741 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga 1801 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt 1861 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca 1921 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt 1981 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt 2041 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga 2101 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt 2161 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc 2221 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa 2281 cctataaaaa taggcgtatc acgaggccct ttcgggtcgc gcgtttcggt gatgacggtg 2341 aaaacctctg acacatgcag ctcccgttga cggtcacagc ttgtctgtaa gcggatgccg 2401 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta 2461 actatgcggc atcagagcag attgtactga gagtgcacca taaaattgta aacgttaata 2521 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caatagaccg 2581 aaatcggcaa aatcccttat aaatcaaaag aatagcccga gatagagttg agtgttgttc 2641 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa 2701 ccgtctatca gggcgatggc ccactacgtg aaccatcacc caaatcaagt tttttggggt 2761 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac 2821 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta 2881 aggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg 2941 cgccgctaca gggcgcgtac tatggttgct ttgacgtatg cggtgtgaaa taccgcacag 3001 atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg 3061 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc 3121 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac 3181 ggccagtgaa ttggaggcta cagtcagtgg agaggacttt cacaggctgt cgccgtgctc 3241 atttgataac tgcccgttat tcatgcgaca ctctagagag cacttccttc tcggcgctgc 3301 accacgtgat gtcctctgag cggatcctcc ccgtgtctgg gtcctctccg ggcatctctc 3361 ctccctcacc caaccccatg ccgtcttcac tcgctgggtt cccttttcct tctccttctg 3421 gggcctgtgc catctctcgt ttcttaggat ggccttctcc gacggatgtc tcccttgcgt 3481 cccgcctccc cttcttgtag gcctgcatca tcaccgtttt tctggacaac cccaaagtac 3541 cccgtctccc tggctttagc cacctctcca tcctcttgct ttctttgcct ggacaccccg 3601 ttctcctgtg gattcgggtc acctctcact cctttcattt gggcagctcc cctacccccc 3661 ttacctctct agtctgtgct agctcttcca gccccctgtc atggcatctt ccaggggtcc 3721 gagagctcag ctagtcttct tcctccaacc cgggccccta tgtccacttc aggacagcac 3781 tagtggacct aataacttct atagcataca ttatacgaag ttatattaag ggttccggat 3841 ctcgaccagc ttctgatgga attagaactt ggcaaaacaa tactgagaat gaagtgtatg 3901 tggaacagag gctgctgatc tcgttcttca ggctatgaaa ctgacacatt tggaaaccac 3961 agtacttaga accacaaagt gggaatcaag agaaaaacaa tgatcccacg agagatctat 4021 agatctatag atcatgagtg ggaggaatga gctggccctt aatttggttt tgcttgttta 4081 aattatgata tccaactatg aaacattatc ataaagcaat agtaaagagc cttcagtaaa 4141 gagcaggcat ttatctaatc ccaccccacc cccacccccg tagctccaat ccttccattc 4201 aaaatgtagg tactctgttc tcacccttct taacaaagta tgacaggaaa aacttccatt 4261 ttagtggaca tctttattgt ttaatagatc atcaatttct gcatcccggg gatctgatat 4321 catcgatgca tggggtcgtg cgctcctttc ggtcgggcgc tgcgggtcgt ggggcgggcg 4381 tcaggcaccg ggcttgcggg tcatgcacca ggtgcgcggt ccttcgggca cctcgacgtc 4441 ggcggtgacg gtgaagccga gccgctcgta gaaggggagg ttgtggggcg cggaggtctc 4501 caggaaggcg ggcaccccgg cgcgctcggc cgcctccact ccggggagca cgacggcgct 4561 gcccagaccc ttgccctggt ggtcgggcga gacgccgacg gtggccagga accacgcggg 4621 ctccttgggc cggtgcggcg ccaggaggcc ttccatctgt tgctgcgcgg ccagccggga 4681 accgctcaac tcggccatgc gcgggccgat ctcggcgaac accgcccccg cttcgacgct 4741 ctccggcgtg gtccagaccg ccaccgcggc gccgtcgtcc gcgacccaca ccttgccgat 4801 gtcgagcccg acgcgcgtga ggaagagttc ttgcagctcg gtgacccgct cgatgtggcg 4861 gtccggatcg acggtgtggc gcgtggcggg gtagtcggcg aacgcggcgg cgagggtgcg 4921 tacggccctg gggacgtcgt cgcgggtggc gaggcgcacc gtgggcttgt actcggtcat 4981 ggtaagcttc agctgctcga gatctagatg gatgcaggtc gaaaggcccg gagatgagga 5041 agaggagaac agcgcggcag acgtgcgctt ttgaagcgtg cagaatgccg ggcctccgga 5101 ggaccttcgg gcgcccgccc cgcccctgag cccgcccctg agcccgcccc cggacccacc 5161 ccttcccagc ctctgagccc agaaagcgaa ggagcaaagc tgctattggc cgctgcccca 5221 aaggcctacc cgcttccatt gctcagcggt gctgtccatc tgcacgagac tggtgagacg 5281 tgctacttcc atttgtcacg tcctgcacga cgcgagctgc ggggcggggg ggaacttcct 5341 gactagggga ggagtagaag gtggcgcgaa ggggccacca aagaacggag tcggttggcg 5401 cctaccggtg gatgtggaat gtgtgcgagg ccagaggcca cttgtgtagc gccaagtgcc 5461 cagcggggct gctaaagcgc atgctccaga ctgccttggg aaaagcgcct cccctacccg 5521 gtagaatttc gaggtcgaga tcctaagctt ggctggacgt aaactcctct tcagactagt 5581 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt 5641 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg 5701 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg 5761 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt 5821 ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg 5881 accttacggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg 5941 gtgatgcggt tttggcagta caccaatggg cgtggatagc ggtttgactc acggggattt 6001 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac 6061 tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg 6121 tgggaggtct atataagcag agctcgttta gtgaaccgtc agaattttgt aatacgactc 6181 actatagggc ggccgggaat tcgtcgactg gatccggtac cgaggagatc tgccgccgcg 6241 atcgccggcg cgccagatct caagcttaac tagctagcgg accgacgcgt acgcggccgc 6301 tcgagcagaa actcatctca gaagaggatc tggcagcaaa tgatatcctg gattacaagg 6361 atgacgacga taaggtttaa acggccggcc gcggtcatag ctgtttcctg aacagatccc 6421 gggtggcatc cctgtgaccc ctccccagtg cctctcctgg ccctggaagt tgccactcca 6481 gtgcccacca gccttgtcct aataaaatta agttgcatca ttttgtctga ctaggtgtcc 6541 ttctataata ttatggggtg gaggggggtg gtatggagca aggggcaagt tgggaagaca 6601 acctgtaggg cctgcggggt ctattgggaa ccaagctgga gtgcagtggc acaatcttgg 6661 ctcactgcaa tctccgcctc ctgggttcaa gcgattctcc tgcctcagcc tcccgagttg 6721 ttgggattcc aggcatgcat gaccaggctc agctaatttt tgtttttttg gtagagacgg 6781 ggtttcacca tattggccag gctggtctcc aactcctaat ctcaggtgat ctacccacct 6841 tggcctccca aattgctggg attacaggcg tgaaccactg ctcccttccc tgtccttctg 6901 attttaaaat aactatacca gcaggaggac gtccagacac agcataggct acctggccat 6961 gcccaaccgg tgccagagag gatcctggga gggagagctt ggcagggggt gggagggaag 7021 ggggggatgc gtgacctgcc cggttctcag tggccaccct gcgctaccct ctcccagaac 7081 ctgagctgct ctgacgcggc cgtctggtgc gtttcactga tcctggtgct gcagcttcct 7141 tacacttccc aagaggagaa gcagtttgga aaaacaaaat cagaataagt tggtcctgag 7201 ttctaacttt ggctcttcac ctttctagtc cccaatttat attgttcctc cgtgcgtcag 7261 ttttacctgt gagataaggc cagtagccag ccccgtcctg gcagggctgt ggtgaggagg 7321 ggggtgtccg tgtggaaaac tccctttgtg agaatggtgc gtcctaggtg ttcaccaggt 7381 cgtggccgcc tctactccct ttctctttct ccatccttct ttccttaaag agtccccagt 7441 gctatctggg acatattcct ccgcccagag cagggtcccg cttccctaag gccctgctct 7501 gtctaga //