LOCUS       .                       7255 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             complement(1256..2116)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ccffcc"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      complement(497..1085)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /label="ori"
     misc_feature    6720..7249
                     /note="right homology arm from the adeno-associated virus
                     integration site (AAVS1) within intron 1 of the human
                     PPP1R12C gene"
                     /note="color: #ffe4c4"
                     /label="HA-R"
     misc_feature    3278..3779
                     /note="left homology arm from the adeno-associated virus
                     integration site (AAVS1) within intron 1 of the human
                     PPP1R12C gene"
                     /note="color: #ffe4c4"
                     /label="HA-L"
     promoter        complement(4768..5267)
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /note="color: #ffffff"
                     /label="PGK promoter"
     polyA_signal    6169..6645
                     /note="human growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
                     /label="hGH poly(A) signal"
     rep_origin      complement(2503..2958)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
                     /label="f1 ori"
     CDS             complement(4328..4726)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /note="confers resistance to blasticidin"
                     /note="color: #ccffcc"
                     /product="blasticidin S deaminase"
                     /transl_table=1
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG*"
                     /label="BSD"
     enhancer        5385..5688
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
                     /label="CMV enhancer"
     promoter        5689..5892
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /note="color: #ffffff"
                     /label="CMV promoter"
     promoter        complement(2117..2221)
                     /gene="bla"
                     /label="AmpR promoter"
                     /note="color: #ffffff"
     CDS             6051..6080
                     /codon_start=1
                     /product="Myc (human c-Myc oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
                     /note="color: #cc99b2"
     CDS             6099..6122
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
                     /note="color: #cc99b2"
     promoter        5918..5936
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff"
                     /label="T7 promoter"
     misc_feature    5945..6048
                     /note="multiple cloning site"
                     /note="color: #99ccff"
                     /label="MCS"
ORIGIN
        1 atcactctcg ccggttggac tttagatcag aagggatctt gctgccgccc gaaagaggaa
       61 gggctggaag aggaaggagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
      121 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
      181 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
      241 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
      301 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
      361 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
      421 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
      481 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
      541 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
      601 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
      661 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
      721 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
      781 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
      841 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
      901 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
      961 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     1021 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     1081 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     1141 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     1201 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     1261 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     1321 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     1381 ctgcaatgat accgcgagaa ccacgctcac cggctccaga tttatcagca ataaaccagc
     1441 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     1501 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     1561 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     1621 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     1681 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     1741 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     1801 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     1861 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     1921 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     1981 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     2041 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     2101 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     2161 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     2221 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
     2281 cctataaaaa taggcgtatc acgaggccct ttcgggtcgc gcgtttcggt gatgacggtg
     2341 aaaacctctg acacatgcag ctcccgttga cggtcacagc ttgtctgtaa gcggatgccg
     2401 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta
     2461 actatgcggc atcagagcag attgtactga gagtgcacca taaaattgta aacgttaata
     2521 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caatagaccg
     2581 aaatcggcaa aatcccttat aaatcaaaag aatagcccga gatagagttg agtgttgttc
     2641 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
     2701 ccgtctatca gggcgatggc ccactacgtg aaccatcacc caaatcaagt tttttggggt
     2761 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
     2821 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
     2881 aggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
     2941 cgccgctaca gggcgcgtac tatggttgct ttgacgtatg cggtgtgaaa taccgcacag
     3001 atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg
     3061 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
     3121 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
     3181 ggccagtgaa ttggaggcta cagtcagtgg agaggacttt cacaggctgt cgccgtgctc
     3241 atttgataac tgcccgttat tcatgcgaca ctctagagag cacttccttc tcggcgctgc
     3301 accacgtgat gtcctctgag cggatcctcc ccgtgtctgg gtcctctccg ggcatctctc
     3361 ctccctcacc caaccccatg ccgtcttcac tcgctgggtt cccttttcct tctccttctg
     3421 gggcctgtgc catctctcgt ttcttaggat ggccttctcc gacggatgtc tcccttgcgt
     3481 cccgcctccc cttcttgtag gcctgcatca tcaccgtttt tctggacaac cccaaagtac
     3541 cccgtctccc tggctttagc cacctctcca tcctcttgct ttctttgcct ggacaccccg
     3601 ttctcctgtg gattcgggtc acctctcact cctttcattt gggcagctcc cctacccccc
     3661 ttacctctct agtctgtgct agctcttcca gccccctgtc atggcatctt ccaggggtcc
     3721 gagagctcag ctagtcttct tcctccaacc cgggccccta tgtccacttc aggacagcac
     3781 tagtggacct aataacttct atagcataca ttatacgaag ttatattaag ggttccggat
     3841 ctcgaccagc ttctgatgga attagaactt ggcaaaacaa tactgagaat gaagtgtatg
     3901 tggaacagag gctgctgatc tcgttcttca ggctatgaaa ctgacacatt tggaaaccac
     3961 agtacttaga accacaaagt gggaatcaag agaaaaacaa tgatcccacg agagatctat
     4021 agatctatag atcatgagtg ggaggaatga gctggccctt aatttggttt tgcttgttta
     4081 aattatgata tccaactatg aaacattatc ataaagcaat agtaaagagc cttcagtaaa
     4141 gagcaggcat ttatctaatc ccaccccacc cccacccccg tagctccaat ccttccattc
     4201 aaaatgtagg tactctgttc tcacccttct taacaaagta tgacaggaaa aacttccatt
     4261 ttagtggaca tctttattgt ttaatagatc atcaatttct gcatcccggg gatctgatat
     4321 catcgattta gccctcccac acataaccag agggcagcaa ttcacgaatc ccaactgccg
     4381 tcggctgtcc atcactgtcc ttcactatgg ctttgatccc aggatgcaga tcgagaagca
     4441 cctgtcggca ccgtccgcag gggctcaaga tgcccctgtt ctcatttccg atcgcgacga
     4501 tacaagtcag gttgccagct gccgcagcag cagcagtgcc cagcaccacg agttctgcac
     4561 aaggtccccc agtaaaatga tatacattga caccagtgaa gatgcggccg tcgctagaga
     4621 gagctgcgct ggcgacgctg tagtcttcag agatggggat gctgttgatt gtagccgttg
     4681 ctctttcaat gagggtggat tcttcttgag acaaaggctt ggccattgta caagcttcag
     4741 ctgctcgaga tctagatgga tgcaggtcga aaggcccgga gatgaggaag aggagaacag
     4801 cgcggcagac gtgcgctttt gaagcgtgca gaatgccggg cctccggagg accttcgggc
     4861 gcccgccccg cccctgagcc cgcccctgag cccgcccccg gacccacccc ttcccagcct
     4921 ctgagcccag aaagcgaagg agcaaagctg ctattggccg ctgccccaaa ggcctacccg
     4981 cttccattgc tcagcggtgc tgtccatctg cacgagactg gtgagacgtg ctacttccat
     5041 ttgtcacgtc ctgcacgacg cgagctgcgg ggcggggggg aacttcctga ctaggggagg
     5101 agtagaaggt ggcgcgaagg ggccaccaaa gaacggagtc ggttggcgcc taccggtgga
     5161 tgtggaatgt gtgcgaggcc agaggccact tgtgtagcgc caagtgccca gcggggctgc
     5221 taaagcgcat gctccagact gccttgggaa aagcgcctcc cctacccggt agaatttcga
     5281 ggtcgagatc ctaagcttgg ctggacgtaa actcctcttc agactagtta ttaatagtaa
     5341 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
     5401 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
     5461 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
     5521 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt
     5581 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac
     5641 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
     5701 tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
     5761 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
     5821 cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
     5881 ataagcagag ctcgtttagt gaaccgtcag aattttgtaa tacgactcac tatagggcgg
     5941 ccgggaattc gtcgactgga tccggtaccg aggagatctg ccgccgcgat cgccggcgcg
     6001 ccagatctca agcttaacta gctagcggac cgacgcgtac gcggccgctc gagcagaaac
     6061 tcatctcaga agaggatctg gcagcaaatg atatcctgga ttacaaggat gacgacgata
     6121 aggtttaaac ggccggccgc ggtcatagct gtttcctgaa cagatcccgg gtggcatccc
     6181 tgtgacccct ccccagtgcc tctcctggcc ctggaagttg ccactccagt gcccaccagc
     6241 cttgtcctaa taaaattaag ttgcatcatt ttgtctgact aggtgtcctt ctataatatt
     6301 atggggtgga ggggggtggt atggagcaag gggcaagttg ggaagacaac ctgtagggcc
     6361 tgcggggtct attgggaacc aagctggagt gcagtggcac aatcttggct cactgcaatc
     6421 tccgcctcct gggttcaagc gattctcctg cctcagcctc ccgagttgtt gggattccag
     6481 gcatgcatga ccaggctcag ctaatttttg tttttttggt agagacgggg tttcaccata
     6541 ttggccaggc tggtctccaa ctcctaatct caggtgatct acccaccttg gcctcccaaa
     6601 ttgctgggat tacaggcgtg aaccactgct cccttccctg tccttctgat tttaaaataa
     6661 ctataccagc aggaggacgt ccagacacag cataggctac ctggccatgc ccaaccggtg
     6721 ccagagagga tcctgggagg gagagcttgg cagggggtgg gagggaaggg ggggatgcgt
     6781 gacctgcccg gttctcagtg gccaccctgc gctaccctct cccagaacct gagctgctct
     6841 gacgcggccg tctggtgcgt ttcactgatc ctggtgctgc agcttcctta cacttcccaa
     6901 gaggagaagc agtttggaaa aacaaaatca gaataagttg gtcctgagtt ctaactttgg
     6961 ctcttcacct ttctagtccc caatttatat tgttcctccg tgcgtcagtt ttacctgtga
     7021 gataaggcca gtagccagcc ccgtcctggc agggctgtgg tgaggagggg ggtgtccgtg
     7081 tggaaaactc cctttgtgag aatggtgcgt cctaggtgtt caccaggtcg tggccgcctc
     7141 tactcccttt ctctttctcc atccttcttt ccttaaagag tccccagtgc tatctgggac
     7201 atattcctcc gcccagagca gggtcccgct tccctaaggc cctgctctgt ctaga
//
