LOCUS       .                       6226 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        4441..4545
                     /gene="bla"
                     /label="AmpR promoter"
                     /note="color: #ffffff"
     repeat_region   3489..3629
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     repeat_region   1..141
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     rep_origin      3704..4159
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
                     /label="f1 ori"
     polyA_signal    2973..3449
                     /note="human growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
                     /label="hGH poly(A) signal"
     rep_origin      5577..6165
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /label="ori"
     misc_feature    2361..2949
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
                     /note="color: #ffffff"
                     /label="WPRE"
     CDS             1640..2335
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /note="color: #05fd14"
                     /product="improved green fluorescent protein from
                     Pontellina plumata (Evdokimov et al., 2006)"
                     /transl_table=1
                     /translation="MESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKM
                     KSTKGALTFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVL
                     HVSFSYRYEAGRVIGDFKVMGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNDLDGSFTR
                     TFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFAFRRVEEDHSNTELGIVEYQHAF
                     KTPDADAGEE"
                     /label="TurboGFP"
     CDS             4546..5406
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ccffcc"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     intron          631..1568
                     /note="intron upstream of the start codon of human EF-1α"
                     /note="color: noColor"
                     /label="EF-1α intron A"
     promoter        400..1577
                     /note="strong constitutive promoter for human elongation
                     factor EF-1α"
                     /note="color: #ffffff"
                     /label="EF-1α promoter"
     misc_feature    1578..1639
                     /note="multiple cloning site"
                     /note="color: #99ccff"
                     /label="MCS"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca accgtctagt tattaatagt aatcaattac
      181 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
      241 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
      301 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
      361 tgcccacttg gcagtacatc aagtgtatca tatgcgtgag gctccggtgc ccgtcagtgg
      421 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
      481 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
      541 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
      601 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
      661 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
      721 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
      781 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
      841 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
      901 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
      961 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     1021 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     1081 gggggtagtc tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg
     1141 ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc
     1201 cgcttcccgg ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg
     1261 cgggtgagtc acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg
     1321 actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctcgagc ttttggagta
     1381 cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg
     1441 tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt
     1501 ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc
     1561 catttcaggt gtcgtgaggt acctcgaaca tcgatccgag gagatctgcc gccgcgatcg
     1621 gcgcgccacg cgtgtcgaca tggagagcga cgagagcggc ctgcccgcca tggagatcga
     1681 gtgccgcatc accggcaccc tgaacggcgt ggagttcgag ctggtgggcg gcggagaggg
     1741 cacccccgag cagggccgca tgaccaacaa gatgaagagc accaaaggcg ccctgacctt
     1801 cagcccctac ctgctgagcc acgtgatggg ctacggcttc taccacttcg gcacctaccc
     1861 cagcggctac gagaacccct tcctgcacgc catcaacaac ggcggctaca ccaacacccg
     1921 catcgagaag tacgaggacg gcggcgtgct gcacgtgagc ttcagctacc gctacgaggc
     1981 cggccgcgtg atcggcgact tcaaggtgat gggcaccggc ttccccgagg acagcgtgat
     2041 cttcaccgac aagatcatcc gcagcaacgc caccgtggag cacctgcacc ccatgggcga
     2101 taacgatctg gatggcagct tcacccgcac cttcagcctg cgcgacggcg gctactacag
     2161 ctccgtggtg gacagccaca tgcacttcaa gagcgccatc caccccagca tcctgcagaa
     2221 cgggggcccc atgttcgcct tccgccgcgt ggaggaggat cacagcaaca ccgagctggg
     2281 catcgtggag taccagcacg ccttcaagac cccggatgca gatgccggtg aagaaagagt
     2341 ttaaacggcc ggccagatct aatcaacctc tggattacaa aatttgtgaa agattgactg
     2401 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     2461 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     2521 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     2581 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     2641 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     2701 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     2761 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     2821 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     2881 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     2941 cctccccgca tcaaacggcc ggccagatct acgggtggca tccctgtgac ccctccccag
     3001 tgcctctcct ggccctggaa gttgccactc cagtgcccac cagccttgtc ctaataaaat
     3061 taagttgcat cattttgtct gactaggtgt ccttctataa tattatgggg tggagggggg
     3121 tggtatggag caaggggcaa gttgggaaga caacctgtag ggcctgcggg gtctattggg
     3181 aaccaagctg gagtgcagtg gcacaatctt ggctcactgc aatctccgcc tcctgggttc
     3241 aagcgattct cctgcctcag cctcccgagt tgttgggatt ccaggcatgc atgaccaggc
     3301 tcagctaatt tttgtttttt tggtagagac ggggtttcac catattggcc aggctggtct
     3361 ccaactccta atctcaggtg atctacccac cttggcctcc caaattgctg ggattacagg
     3421 cgtgaaccac tgctcccttc cctgtccttc tgattttgta ggtaaccacg tgcggaccga
     3481 gcggccgcag gaacccctag tgatggagtt ggccactccc tctctgcgcg ctcgctcgct
     3541 cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg cggcctcagt
     3601 gagcgagcga gcgcgcagct gcctgcaggg gcgcctgatg cggtattttc tccttacgca
     3661 tctgtgcggt atttcacacc gcatacgtca aagcaaccat agtacgcgcc ctgtagcggc
     3721 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     3781 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     3841 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     3901 gaccccaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     3961 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     4021 ggaacaacac tcaactctat ctcgggctat tcttttgatt tataagggat tttgccgatt
     4081 tcggtctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     4141 atattaacgt ttacaatttt atggtgcact ctcagtacaa tctgctctga tgccgcatag
     4201 ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     4261 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     4321 tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag
     4381 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     4441 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     4501 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     4561 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     4621 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     4681 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     4741 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     4801 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     4861 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     4921 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     4981 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     5041 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     5101 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     5161 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     5221 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     5281 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     5341 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     5401 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     5461 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     5521 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     5581 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     5641 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     5701 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     5761 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     5821 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     5881 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     5941 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     6001 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     6061 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     6121 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     6181 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgt
//
