LOCUS       .                       7117 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             748..3853
                     /codon_start=1
                     /transl_table=1
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAK"
                     /label="Cas9-Up"
                     /note="color: #993366"
     CDS             5081..5941
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ccffcc"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      6112..6700
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /label="ori"
     rep_origin      4239..4694
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
                     /label="f1 ori"
     enhancer        210..513
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
                     /label="CMV enhancer"
     promoter        514..717
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /note="color: #ffffff"
                     /label="CMV promoter"
     repeat_region   1..141
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     repeat_region   3860..4000
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     promoter        4976..5080
                     /gene="bla"
                     /label="AmpR promoter"
                     /note="color: #ffffff"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tatcgatatc aagctttaat agtaatcaat tacggggtca
      181 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
      241 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
      301 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
      361 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
      421 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
      481 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
      541 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
      601 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
      661 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctggt
      721 ttagtggata tccttaaggc cgccaccatg gacaagaagt acagcatcgg cctggacatc
      781 ggtaccaaca gcgtgggctg ggccgtgatc accgacgagt acaaggtgcc cagcaagaag
      841 ttcaaggtgc tgggcaacac cgaccgccac agcatcaaga agaacctgat cggcgccctg
      901 ctgttcgaca gcggcgagac cgccgaggcc acccgcctga agcgcaccgc ccgccgccgc
      961 tacacccgcc gcaagaaccg catctgctac ctgcaggaga tcttcagcaa cgagatggcc
     1021 aaggtggacg acagcttctt ccaccgcctg gaggagagct tcctggtgga ggaggacaag
     1081 aagcacgagc gccaccccat cttcggcaac atcgtggacg aggtggccta ccacgagaag
     1141 taccccacca tctaccacct gcgcaagaag ctggtggaca gcaccgacaa ggccgacctg
     1201 cgcctgatct acctggccct ggcccacatg atcaagttcc gcggccactt cctgatcgag
     1261 ggcgacctga accccgacaa cagcgacgtg gacaagctgt tcatccagct ggtgcagacc
     1321 tacaaccagc tgttcgagga gaaccccatc aacgccagcg gcgtggacgc caaggccatc
     1381 ctgagcgccc gcctgagcaa gagccgccgc ctggagaacc tgatcgccca gctgcccggc
     1441 gagaagaaga acggcctgtt cggcaacctg atcgccctga gcctgggcct gacccccaac
     1501 ttcaagagca acttcgacct ggccgaggac gccaagctgc agctgagcaa ggacacctac
     1561 gacgacgacc tggacaacct gctggcccag atcggcgacc agtacgccga cctgttcctg
     1621 gccgccaaga acctgagcga cgccatcctg ctgagcgaca tcctgcgcgt gaacaccgag
     1681 atcaccaagg cccccctgag cgccagcatg atcaagcgct acgacgagca ccaccaggac
     1741 ctgaccctgc tgaaggccct ggtgcgccag cagctgcccg agaagtacaa ggagatcttc
     1801 ttcgaccaga gcaagaacgg ctacgccggc tacatcgacg gcggcgccag ccaggaggag
     1861 ttctacaagt tcatcaagcc catcctggag aagatggacg gcaccgagga gctgctggtg
     1921 aagctgaacc gcgaggacct gctgcgcaag cagcgcacct tcgacaacgg cagcatcccc
     1981 caccagatcc acctgggcga gctgcacgcc atcctgcgcc gccaggagga cttctacccc
     2041 ttcctgaagg acaaccgcga gaagatcgag aagatcctga ccttccgcat cccctactac
     2101 gtgggccccc tggcccgcgg caacagccgc ttcgcctgga tgacccgcaa gagcgaggag
     2161 accatcaccc cctggaactt cgaggaggtg gtggacaagg gcgccagcgc ccagagcttc
     2221 atcgagcgca tgaccaactt cgacaagaac ctgcccaacg agaaggtgct gcccaagcac
     2281 agcctgctgt acgagtactt caccgtgtac aacgagctga ccaaggtgaa gtacgtgacc
     2341 gagggcatgc gcaagcccgc cttcctgagc ggcgagcaga agaaggccat cgtggacctg
     2401 ctgttcaaga ccaaccgcaa ggtgaccgtg aagcagctga aggaggacta cttcaagaag
     2461 atcgagtgct tcgacagcgt ggagatcagc ggcgtggagg accgcttcaa cgccagcctg
     2521 ggcacctacc acgacctgct gaagatcatc aaggacaagg acttcctgga caacgaggag
     2581 aacgaggaca tcctggagga catcgtgctg accctgaccc tgttcgagga ccgcgagatg
     2641 atcgaggagc gcctgaagac ctacgcccac ctgttcgacg acaaggtgat gaagcagctg
     2701 aagcgccgcc gctacaccgg ctggggccgc ctgagccgca agcttatcaa cggcatccgc
     2761 gacaagcaga gcggcaagac catcctggac ttcctgaaga gcgacggctt cgccaaccgc
     2821 aacttcatgc agctgatcca cgacgacagc ctgaccttca aggaggacat ccagaaggcc
     2881 caggtgagcg gccagggcga cagcctgcac gagcacatcg ccaacctggc cggcagcccc
     2941 gccatcaaga agggcatcct gcagaccgtg aaggtggtgg acgagctggt gaaggtgatg
     3001 ggccgccaca agcccgagaa catcgtgatc gagatggccc gcgagaacca gaccacccag
     3061 aagggccaga agaacagccg cgagcgcatg aagcgcatcg aggagggcat caaggagctg
     3121 ggcagccaga tcctgaagga gcaccccgtg gagaacaccc agctgcagaa cgagaagctg
     3181 tacctgtact acctgcagaa cggccgcgac atgtacgtgg accaggagct ggacatcaac
     3241 cgcctgagcg actacgacgt ggaccacatc gtgccccaga gcttcctgaa ggacgacagc
     3301 atcgacaaca aggtgctgac ccgcagcgac aagaaccgcg gcaagagcga caacgtgccc
     3361 agcgaggagg tggtgaagaa gatgaagaac tactggcgcc agctgctgaa cgccaagctg
     3421 atcacccagc gcaagttcga caacctgacc aaggccgagc gcggcggcct gagcgagctg
     3481 gacaaggccg gcttcatcaa gcgccagctg gtggagaccc gccagatcac caagcacgtg
     3541 gcccagatcc tggacagccg catgaacacc aagtacgacg agaacgacaa gctgatccgc
     3601 gaggtgaagg tgatcaccct gaagagcaag ctggtgagcg acttccgcaa ggacttccag
     3661 ttctacaagg tgcgcgagat caacaactac caccacgccc acgacgccta cctgaacgcc
     3721 gtggtgggca ccgccctgat caagaagtac cccaagctgg agagcgagtt cgtgtacggc
     3781 gactacaagg tgtacgacgt gcgcaagatg atcgccaaga gcgagcagga gatcggcaag
     3841 gccaccgcca agtagatcta ggaaccccta gtgatggagt tggccactcc ctctctgcgc
     3901 gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg
     3961 gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg cagcttggca ctggccgtcg
     4021 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
     4081 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
     4141 agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt
     4201 gcggtatttc acaccgcata cgtcaaagca accatagtac gcgccctgta gcggcgcatt
     4261 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     4321 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     4381 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     4441 caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     4501 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     4561 aacactcaac cctatctcgg gctattcttt tgatttataa gggattttgc cgatttcggc
     4621 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     4681 aacgtttaca attttatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     4741 ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     4801 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
     4861 gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     4921 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     4981 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     5041 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     5101 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     5161 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     5221 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     5281 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     5341 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     5401 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     5461 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     5521 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     5581 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     5641 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     5701 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     5761 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     5821 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     5881 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     5941 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     6001 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     6061 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     6121 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     6181 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     6241 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     6301 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     6361 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     6421 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     6481 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     6541 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     6601 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     6661 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     6721 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     6781 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     6841 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
     6901 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
     6961 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
     7021 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
     7081 tcacacagga aacagctatg accatgatta cgaattg
//
