LOCUS       .                       4754 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             1453..1476
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
                     /note="color: #cc99b2"
     CDS             1405..1434
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
                     /note="color: #cc99b2"
     promoter        2969..3073
                     /gene="bla"
                     /label="AmpR promoter"
                     /note="color: #ffffff"
     repeat_region   2017..2157
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     repeat_region   1..141
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     promoter        521..724
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /note="color: #ffffff"
                     /label="CMV promoter"
     enhancer        217..520
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
                     /label="CMV enhancer"
     rep_origin      2232..2687
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
                     /label="f1 ori"
     polyA_signal    1501..1977
                     /note="human growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
                     /label="hGH poly(A) signal"
     rep_origin      4105..4693
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /label="ori"
     CDS             3074..3934
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ccffcc"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     intron          873..1251
                     /note="fusion between human cytomegalovirus intron A and
                     human β-globin intron 2"
                     /note="color: noColor"
                     /label="chimeric intron"
     misc_feature    1324..1408
                     /note="multiple cloning site"
                     /note="color: #99ccff"
                     /label="MCS"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca accgtctagt tattaatagt aatcaattac
      181 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
      241 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
      301 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
      361 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
      421 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
      481 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta
      541 catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga
      601 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa
      661 ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag
      721 agctcgttta gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca
      781 tagaagacac cgggaccgat ccagcctccg cggattcgaa tcccggccgg gaacggtgca
      841 ttggaacgcg gattccccgt gccaagagtg acgtaagtac cgcctataga gtctataggc
      901 ccacaaaaaa tgctttcttc ttttaatata cttttttgtt tatcttattt ctaatacttt
      961 ccctaatctc tttctttcag ggcaataatg atacaatgta tcatgcctct ttgcaccatt
     1021 ctaaagaata acagtgataa tttctgggtt aaggcaatag caatatttct gcatataaat
     1081 atttctgcat ataaattgta actgatgtaa gaggtttcat attgctaata gcagctacaa
     1141 tccagctacc attctgcttt tattttatgg ttgggataag gctggattat tctgagtcca
     1201 agctaggccc ttttgctaat catgttcata cctcttatct tcctcccaca gctcctgggc
     1261 aacgtgctgg tctgtgtgct ggcccatcac tttggcaaag aattgggatt cgaacatcga
     1321 tccgaggaga tctgccgccg cgatcgccgg cgcgccagat ctcaagctta actagctagc
     1381 ggaccgacgc gtacgcggcc gctcgagcag aaactcatct cagaagagga tctggcagca
     1441 aatgatatcc tggattacaa ggatgacgac gataaggttt aaacggccgg ccggatctac
     1501 gggtggcatc cctgtgaccc ctccccagtg cctctcctgg ccctggaagt tgccactcca
     1561 gtgcccacca gccttgtcct aataaaatta agttgcatca ttttgtctga ctaggtgtcc
     1621 ttctataata ttatggggtg gaggggggtg gtatggagca aggggcaagt tgggaagaca
     1681 acctgtaggg cctgcggggt ctattgggaa ccaagctgga gtgcagtggc acaatcttgg
     1741 ctcactgcaa tctccgcctc ctgggttcaa gcgattctcc tgcctcagcc tcccgagttg
     1801 ttgggattcc aggcatgcat gaccaggctc agctaatttt tgtttttttg gtagagacgg
     1861 ggtttcacca tattggccag gctggtctcc aactcctaat ctcaggtgat ctacccacct
     1921 tggcctccca aattgctggg attacaggcg tgaaccactg ctcccttccc tgtccttctg
     1981 attttgtagg taaccacgtg cggaccgagc ggccgcagga acccctagtg atggagttgg
     2041 ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac
     2101 gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc gcgcagctgc ctgcaggggc
     2161 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa
     2221 gcaaccatag tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     2281 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     2341 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     2401 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc
     2461 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     2521 ctttaatagt ggactcttgt tccaaactgg aacaacactc aactctatct cgggctattc
     2581 ttttgattta taagggattt tgccgatttc ggtctattgg ttaaaaaatg agctgattta
     2641 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaattttat ggtgcactct
     2701 cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc
     2761 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
     2821 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa
     2881 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
     2941 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     3001 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     3061 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     3121 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     3181 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     3241 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     3301 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     3361 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     3421 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     3481 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     3541 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     3601 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     3661 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     3721 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     3781 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     3841 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     3901 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     3961 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     4021 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     4081 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     4141 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     4201 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     4261 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     4321 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     4381 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     4441 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     4501 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     4561 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     4621 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     4681 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     4741 ttttgctcac atgt
//
