LOCUS       .                       5725 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        3932..4036
                     /gene="bla"
                     /label="AmpR promoter"
                     /note="color: #ffffff"
     repeat_region   2980..3120
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /note="color: #ffe4c4"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     promoter        854..1057
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /note="color: #ffffff"
                     /label="CMV promoter"
     enhancer        474..853
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
                     /label="CMV enhancer"
     rep_origin      3195..3650
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
                     /label="f1 ori"
     polyA_signal    2464..2940
                     /note="human growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
                     /label="hGH poly(A) signal"
     rep_origin      5068..5656
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /label="ori"
     CDS             1741..2436
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /note="color: #05fd14"
                     /product="improved green fluorescent protein from
                     Pontellina plumata (Evdokimov et al., 2006)"
                     /transl_table=1
                     /translation="MESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKM
                     KSTKGALTFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVL
                     HVSFSYRYEAGRVIGDFKVMGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNDLDGSFTR
                     TFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFAFRRVEEDHSNTELGIVEYQHAF
                     KTPDADAGEE"
                     /label="TurboGFP"
     CDS             4037..4897
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ccffcc"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     intron          1206..1584
                     /note="fusion between human cytomegalovirus intron A and
                     human β-globin intron 2"
                     /note="color: noColor"
                     /label="chimeric intron"
     misc_feature    1673..1740
                     /note="multiple cloning site"
                     /note="color: #99ccff"
                     /label="MCS"
ORIGIN
        1 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
       61 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
      121 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg cgcgctcgct
      181 cgctcactga ggccgcccgg gcaaagcccg ggcgtcgggc gacctttggt cgcccggcct
      241 cagtgagcga gcgagcgcgc agagagggag tggccaactc catcactagg ggttccttgt
      301 agttaatgat taacccgcca tgctacttat ctacgtagcc atgctctagc ggcctcggcc
      361 tctgcataaa taaaaaaaat tagtcagcca tgagcttggc ccattgcata cgttgtatcc
      421 atatcataat atgtacattt atattggctc atgtccaaca ttaccgccat gttgacattg
      481 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
      541 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
      601 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
      661 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
      721 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
      781 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
      841 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
      901 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
      961 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
     1021 taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc
     1081 ctggagacgc catccacgct gttttgacct ccatagaaga caccgggacc gatccagcct
     1141 ccgcggattc gaatcccggc cgggaacggt gcattggaac gcggattccc cgtgccaaga
     1201 gtgacgtaag taccgcctat agagtctata ggcccacaaa aaatgctttc ttcttttaat
     1261 atactttttt gtttatctta tttctaatac tttccctaat ctctttcttt cagggcaata
     1321 atgatacaat gtatcatgcc tctttgcacc attctaaaga ataacagtga taatttctgg
     1381 gttaaggcaa tagcaatatt tctgcatata aatatttctg catataaatt gtaactgatg
     1441 taagaggttt catattgcta atagcagcta caatccagct accattctgc ttttatttta
     1501 tggttgggat aaggctggat tattctgagt ccaagctagg cccttttgct aatcatgttc
     1561 atacctctta tcttcctccc acagctcctg ggcaacgtgc tggtctgtgt gctggcccat
     1621 cactttggca aagaattggg attcgaacat cgatccgagg agatctgccg ccgcgatcgc
     1681 cggcgcgcca gatctcaagc ttaactagtt agcggaccga cgcgtacgcg gccgctcgag
     1741 atggagagcg acgagagcgg cctgcccgcc atggagatcg agtgccgcat caccggcacc
     1801 ctgaacggcg tggagttcga gctggtgggc ggcggagagg gcacccccga gcagggccgc
     1861 atgaccaaca agatgaagag caccaaaggc gccctgacct tcagccccta cctgctgagc
     1921 cacgtgatgg gctacggctt ctaccacttc ggcacctacc ccagcggcta cgagaacccc
     1981 ttcctgcacg ccatcaacaa cggcggctac accaacaccc gcatcgagaa gtacgaggac
     2041 ggcggcgtgc tgcacgtgag cttcagctac cgctacgagg ccggccgcgt gatcggcgac
     2101 ttcaaggtga tgggcaccgg cttccccgag gacagcgtga tcttcaccga caagatcatc
     2161 cgcagcaacg ccaccgtgga gcacctgcac cccatgggcg ataacgatct ggatggcagc
     2221 ttcacccgca ccttcagcct gcgcgacggc ggctactaca gctccgtggt ggacagccac
     2281 atgcacttca agagcgccat ccaccccagc atcctgcaga acgggggccc catgttcgcc
     2341 ttccgccgcg tggaggagga tcacagcaac accgagctgg gcatcgtgga gtaccagcac
     2401 gccttcaaga ccccggatgc agatgccggt gaagaaagag tttaaacggc cggccagatc
     2461 tacgggtggc atccctgtga cccctcccca gtgcctctcc tggccctgga agttgccact
     2521 ccagtgccca ccagccttgt cctaataaaa ttaagttgca tcattttgtc tgactaggtg
     2581 tccttctata atattatggg gtggaggggg gtggtatgga gcaaggggca agttgggaag
     2641 acaacctgta gggcctgcgg ggtctattgg gaaccaagct ggagtgcagt ggcacaatct
     2701 tggctcactg caatctccgc ctcctgggtt caagcgattc tcctgcctca gcctcccgag
     2761 ttgttgggat tccaggcatg catgaccagg ctcagctaat ttttgttttt ttggtagaga
     2821 cggggtttca ccatattggc caggctggtc tccaactcct aatctcaggt gatctaccca
     2881 ccttggcctc ccaaattgct gggattacag gcgtgaacca ctgctccctt ccctgtcctt
     2941 ctgattttgt aggtaaccac gtgcggaccg agcggccgca ggaaccccta gtgatggagt
     3001 tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc
     3061 gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg
     3121 ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgtc
     3181 aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     3241 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     3301 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     3361 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt tgggtgatgg
     3421 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     3481 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaactcta tctcgggcta
     3541 ttcttttgat ttataaggga ttttgccgat ttcggtctat tggttaaaaa atgagctgat
     3601 ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt tatggtgcac
     3661 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc
     3721 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     3781 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg
     3841 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
     3901 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     3961 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     4021 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     4081 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     4141 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     4201 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     4261 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     4321 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     4381 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     4441 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     4501 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     4561 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     4621 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     4681 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     4741 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     4801 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     4861 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     4921 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     4981 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     5041 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     5101 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     5161 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     5221 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     5281 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     5341 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     5401 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     5461 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     5521 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     5581 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     5641 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     5701 gccttttgct cacatgttct ttcct
//
