LOCUS       .                       9803 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_RNA        1..76
                     /note="guide RNA scaffold for the Streptococcus pyogenes
                     CRISPR/Cas9 system"
                     /note="color: #00ccff"
                     /label="gRNA scaffold"
     CDS             7408..7428
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /note="color: #993366"
                     /product="nuclear localization signal of SV40 large T
                     antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     CDS             3250..3270
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /note="color: #993366"
                     /product="nuclear localization signal of SV40 large T
                     antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     CDS             3205..3246
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /transl_table=1
                     /translation="GKPIPNPLLGLDST"
                     /label="V5 tag"
                     /note="color: #cc99b2"
     polyA_signal    8856..8904
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
                     /note="color: #a6acb3"
                     /label="HSV TK poly(A) signal"
     CDS             7450..7503
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /note="color: #cc99b2"
                     /product="2A peptide from Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     promoter        complement(2148..2252)
                     /gene="bla"
                     /label="AmpR promoter"
                     /note="color: #ffffff"
     promoter        2898..3101
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /note="color: #ffffff"
                     /label="CMV promoter"
     enhancer        2518..2897
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
                     /label="CMV enhancer"
     rep_origin      9106..9534
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
                     /label="f1 ori"
     rep_origin      complement(528..1116)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /label="ori"
     CDS             complement(1287..2147)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ccffcc"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     CDS             3295..7395
                     /codon_start=1
                     /locus_tag=""
                     /note="generates RNA-guided double strand breaks in DNA"
                     /note="color: #993366"
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus
                     pyogenes Type II CRISPR/Cas system"
                     /protein_id=""
                     /transl_table=1
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
                     /label="Cas9"
     CDS             7504..8775
                     /codon_start=1
                     /product="truncated human CD4 cell surface glycoprotein
                     lacking the cytoplasmic tail"
                     /transl_table=1
                     /translation="MNRGVPFRHLLLVLQLALLPAATQG,KKVVLGKKGDTVELTCTAS
                     QKKSIQFHWKNSNQIKILGNQGSFLTKGPSKLNDRADSRRSLWDQGNFPLIIKNLKIED
                     SDTYICEVEDQKEEVQLLVFGLTANSDTHLLQGQSLTLTLESPPGSSPSVQCRSPRGKN
                     IQGGKTLSVSQLELQDSGTWTCTVLQNQKKVEFKIDIVVLAFQKASSIVYKKEGEQVEF
                     SFPLAFTVEKLTGSGELWWQAERASSSKSWITFDLKNKEVSVKRVTQDPKLQMGKKLPL
                     HLTLPQALPQYAGSGNLTLALEAKTGKLHQEVNLVVMRATQLQKNLTCEVWGPTSPKLM
                     LSLKLENKEAKVSKREKAVWVLNPEAGMWQCLLSDSGQVLLESNIKVLPTWSTPVQPMA
                     LIVLGGVAGLLLFIGLGIFFCVRCRH"
                     /label="CD4 (truncated)"
                     /note="color: #993366"
     terminator      77..82
                     /note="RNA polymerase III transcription terminator"
                     /note="color: #ffffff"
                     /label="polIII terminator"
     CDS             3202..3204
                     /codon_start=1
                     /product="start codon"
                     /transl_table=1
                     /translation="M"
                     /label="ATG"
                     /note="color: #993366"
     promoter        9554..9802
                     /note="RNA polymerase III promoter for human U6 snRNA
                     (Domitrovich & Kunkel, 2003)"
                     /note="color: #ffffff"
                     /label="U6 promoter"
ORIGIN
        1 gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt
       61 ggcaccgagt cggtgctttt ttctagtata ccgtcgacct ctagctagag cttggcgtaa
      121 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
      181 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
      241 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
      301 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
      361 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
      421 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
      481 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
      541 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
      601 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
      661 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
      721 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
      781 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
      841 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
      901 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
      961 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     1021 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     1081 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     1141 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     1201 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     1261 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     1321 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     1381 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     1441 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     1501 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     1561 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     1621 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     1681 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     1741 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     1801 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     1861 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     1921 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     1981 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     2041 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     2101 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     2161 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     2221 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     2281 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
     2341 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
     2401 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
     2461 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
     2521 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
     2581 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
     2641 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
     2701 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
     2761 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
     2821 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     2881 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
     2941 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     3001 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
     3061 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgtcaga
     3121 tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg gaccgatcca
     3181 gcctccggag cggccgccac catgggcaag cccatcccta accccctgtt ggggctggac
     3241 agcaccgctc ccaaaaagaa aaggaaggtg ggcattcacg gcgtgcctgc ggccgacaaa
     3301 aagtacagca tcggccttga tatcggcacc aatagcgtgg gctgggccgt tatcacagac
     3361 gaatacaagg tacccagcaa gaagttcaag gtgctgggga atacagacag gcactctatc
     3421 aagaaaaacc ttatcggggc tctgctgttt gactcaggcg agaccgccga ggccaccagg
     3481 ttgaagagga ccgcaaggcg aaggtacacc cggaggaaga acaggatctg ctatctgcag
     3541 gagatcttca gcaacgagat ggccaaggtg gacgacagct tcttccacag gctggaggag
     3601 agcttccttg tcgaggagga taagaagcac gaacgacacc ccatcttcgg caacatagtc
     3661 gacgaggtcg cttatcacga gaagtacccc accatctacc acctgcgaaa gaaattggtg
     3721 gatagcaccg ataaagccga cttgcgactt atctacttgg ctctggcgca catgattaag
     3781 ttcaggggcc acttcctgat cgagggcgac cttaaccccg acaacagtga cgtagacaaa
     3841 ttgttcatcc agcttgtaca gacctataac cagctgttcg aggaaaaccc tattaacgcc
     3901 agcggggtgg atgcgaaggc catacttagc gccaggctga gcaaaagcag gcgcttggag
     3961 aacctgatag cccagctgcc cggtgaaaag aagaacggcc tcttcggtaa tctgattgcc
     4021 ctgagcctgg gcctgacccc caacttcaag agcaacttcg acctggcaga agatgccaag
     4081 ctgcagttga gtaaggacac ctatgacgac gacttggaca atctgctcgc ccaaatcggc
     4141 gaccagtacg ctgacctgtt cctcgccgcc aagaaccttt ctgacgcaat cctgcttagc
     4201 gatatcctta gggtgaacac agagatcacc aaggcccccc tgagcgccag catgatcaag
     4261 aggtacgacg agcaccatca ggacctgacc cttctgaagg ccctggtgag gcagcaactg
     4321 cccgagaagt acaaggagat ctttttcgac cagagcaaga acggctacgc cggctacatc
     4381 gacggcggag ccagccaaga ggagttctac aagttcatca agcccatcct ggagaagatg
     4441 gatggcaccg aggagctgct ggtgaagctg aacagggaag atttgctccg gaagcagagg
     4501 acctttgaca acggtagcat cccccaccag atccacctgg gcgagctgca cgcaatactg
     4561 aggcgacagg aggatttcta ccccttcctc aaggacaata gggagaaaat cgaaaagatt
     4621 ctgaccttca ggatccccta ctacgtgggc cctcttgcca ggggcaacag ccgattcgct
     4681 tggatgacaa gaaagagcga ggagaccatc accccctgga acttcgagga agtggtggac
     4741 aaaggagcaa gcgcgcagtc tttcatcgaa cggatgacca atttcgacaa aaacctgcct
     4801 aacgagaagg tgctgcccaa gcacagcctg ctttacgagt acttcaccgt gtacaacgag
     4861 ctcaccaagg tgaaatatgt gaccgagggc atgcgaaaac ccgctttcct gagcggcgag
     4921 cagaagaagg ccatcgtgga cctgctgttc aagaccaaca ggaaggtgac cgtgaagcag
     4981 ctgaaggagg actacttcaa gaagatcgag tgctttgata gcgtggaaat aagcggcgtg
     5041 gaggacaggt tcaacgccag cctgggcacc taccacgact tgttgaagat aatcaaagac
     5101 aaggatttcc tggataatga ggagaacgag gatatactcg aggacatcgt gctgactttg
     5161 accctgtttg aggaccgaga gatgattgaa gaaaggctca aaacctacgc ccacctgttc
     5221 gacgacaaag tgatgaaaca actgaagaga cgaagataca ccggctgggg cagactgtcc
     5281 aggaagctca tcaacggcat tagggacaag cagagcggca agaccatcct ggatttcctg
     5341 aagtccgacg gcttcgccaa ccgaaacttc atgcagctga ttcacgatga cagcttgacc
     5401 ttcaaggagg acatccagaa ggcccaggtt agcggccagg gcgactccct gcacgaacat
     5461 attgcaaacc tggcaggctc ccctgcgatc aagaagggca tactgcagac cgttaaggtt
     5521 gtggacgaat tggtcaaggt catgggcagg cacaagcccg aaaacatagt tatagagatg
     5581 gccagagaga accagaccac ccaaaagggc cagaagaaca gccgggagcg catgaaaagg
     5641 atcgaggagg gtatcaagga actcggaagc cagatcctca aagagcaccc cgtggagaat
     5701 acccagctcc agaacgagaa gctgtacctg tactacctgc agaacggcag ggacatgtac
     5761 gttgaccagg agttggacat caacaggctt tcagactatg acgtggatca catagtgccc
     5821 cagagctttc ttaaagacga tagcatcgac aacaaggtcc tgacccgctc cgacaaaaac
     5881 aggggcaaaa gcgacaacgt gccaagcgaa gaggtggtta aaaagatgaa gaactactgg
     5941 aggcaactgc tcaacgcgaa attgatcacc cagagaaagt tcgataacct gaccaaggcc
     6001 gagaggggcg gactctccga acttgacaaa gcgggcttca taaagaggca gctggtcgag
     6061 acccgacaga tcacgaagca cgtggcccaa atcctcgaca gcagaatgaa taccaagtac
     6121 gatgagaatg acaaactcat cagggaagtg aaagtgatta ccctgaagag caagttggtg
     6181 tccgactttc gcaaagattt ccagttctac aaggtgaggg agatcaacaa ctaccaccat
     6241 gcccacgacg catacctgaa cgccgtggtc ggcaccgccc tgattaagaa gtatccaaag
     6301 ctggagtccg aatttgtcta cggcgactac aaagtttacg atgtgaggaa gatgatcgct
     6361 aagagcgaac aggagatcgg caaggccacc gctaagtatt tcttctacag caacatcatg
     6421 aactttttca agaccgagat cacacttgcc aacggcgaaa tcaggaagag gccgcttatc
     6481 gagaccaacg gtgagaccgg cgagatcgtg tgggacaagg gcagggactt cgccaccgtg
     6541 aggaaagtcc tgagcatgcc ccaggtgaat attgtgaaaa aaactgaggt gcagacaggc
     6601 ggctttagca aggaatccat cctgcccaag aggaacagcg acaagctgat cgcccggaag
     6661 aaggactggg accctaagaa gtatggaggc ttcgacagcc ccaccgtagc ctacagcgtg
     6721 ctggtggtcg cgaaggtaga gaaggggaag agcaagaaac tgaagagcgt gaaggagctg
     6781 ctcggcataa ccatcatgga gaggtccagc tttgagaaga accccattga ctttttggaa
     6841 gccaagggct acaaagaggt caaaaaggac ctgatcatca aactccccaa gtactccctg
     6901 tttgaattgg agaacggcag aaagaggatg ctggcgagcg ctggggaact gcaaaagggc
     6961 aacgaactgg cgctgcccag caagtacgtg aattttctgt acctggcgtc ccactacgaa
     7021 aagctgaaag gcagccccga ggacaacgag cagaagcagc tgttcgtgga gcagcacaag
     7081 cattacctgg acgagataat cgagcaaatc agcgagttca gcaagagggt gattctggcc
     7141 gacgcgaacc tggataaggt cctcagcgcc tacaacaagc accgagacaa acccatcagg
     7201 gagcaggccg agaatatcat acacctgttc accctgacaa atctgggcgc acctgcggca
     7261 ttcaaatact tcgataccac catcgacagg aaaaggtaca ctagcactaa ggaggtgctg
     7321 gatgccacct tgatccacca gtccattacc ggcctgtatg agaccaggat cgacctgagc
     7381 cagcttggag gcgactctag ggcggaccca aaaaagaaaa ggaaggtgga attctctaga
     7441 ggcagtggag agggcagagg aagtctgcta acatgcggtg acgtcgagga gaatcctggc
     7501 ccaatgaacc ggggagtccc ttttaggcac ttgcttctgg tgctgcaact ggcgctcctc
     7561 ccagcagcca ctcagggaaa gaaagtggtg ctgggcaaaa aaggggatac agtggaactg
     7621 acctgtacag cttcccagaa gaagagcata caattccact ggaaaaactc caaccagata
     7681 aagattctgg gaaatcaggg ctccttctta actaaaggtc catccaagct gaatgatcgc
     7741 gctgactcaa gaagaagcct ttgggaccaa ggaaacttcc ccctgatcat caagaatctt
     7801 aagatagaag actcagatac ttacatctgt gaagtggagg accagaagga ggaggtgcaa
     7861 ttgctagtgt tcggattgac tgccaactct gacacccacc tgcttcaggg gcagagcctg
     7921 accctgacct tggagagccc ccctggtagt agcccctcag tgcaatgtag gagtccaagg
     7981 ggtaaaaaca tacagggggg gaagaccctc tccgtgtctc agctggagct ccaggatagt
     8041 ggcacctgga catgcactgt cttgcagaac cagaagaagg tggagttcaa aatagacatc
     8101 gtggtgctag ctttccagaa ggcctccagc atagtctata agaaagaggg ggaacaggtg
     8161 gagttctcct tcccactcgc ctttacagtt gaaaagctga cgggcagtgg cgagctgtgg
     8221 tggcaggcgg agagggcttc ctcctccaag tcttggatca cctttgacct gaagaacaag
     8281 gaagtgtctg taaaacgggt tacccaggac cctaagctcc agatgggcaa gaagctcccg
     8341 ctccacctca ccctgcccca ggccttgcct cagtatgctg gctctggaaa cctcaccctg
     8401 gcccttgaag cgaaaacagg aaagttgcat caggaagtga acctggtggt gatgagagcc
     8461 actcagctcc agaaaaattt gacctgtgag gtgtggggac ccacctcccc taagctgatg
     8521 ctgagcttga aactggagaa caaggaggca aaggtctcga agcgggagaa ggcggtgtgg
     8581 gtgctgaacc ctgaggcggg gatgtggcag tgtctgctga gtgactcggg acaggtcctg
     8641 ctggaatcca acatcaaggt tctgcccaca tggtcgaccc cggtgcagcc aatggccctg
     8701 attgtgctgg ggggcgtcgc cggcctcctg cttttcattg ggctaggcat cttcttctgt
     8761 gtcaggtgcc ggcacaccgg ttagtaatga gtttaaacgg gggaggctaa ctgaaacacg
     8821 gaaggagaca ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac
     8881 gcacgggtgt tgggtcgttt gttcataaac gcggggttcg gtcccagggc tggcactctg
     8941 tcgatacccc accgagaccc cattggggcc aatacgcccg cgtttcttcc ttttccccac
     9001 cccacccccc aagttcgggt gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc
     9061 ctgccatagc agatctgcgc agctggggct ctagggggta tccccacgcg ccctgtagcg
     9121 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     9181 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     9241 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     9301 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     9361 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     9421 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     9481 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattaa
     9541 ggtcgggcag gaagagggcc tatttcccat gattccttca tatttgcata tacgatacaa
     9601 ggctgttaga gagataatta gaattaattt gactgtaaac acaaagatat tagtacaaaa
     9661 tacgtgacgt agaaagtaat aatttcttgg gtagtttgca gttttaaaat tatgttttaa
     9721 aatggactat catatgctta ccgtaacttg aaagtatttc gatttcttgg ctttatatat
     9781 cttgtggaaa ggacgaaaca ccg
//
