LOCUS       .                       2746 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             complement(1686..2546)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ccffcc"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      complement(927..1515)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
                     /label="ori"
     CDS             complement(146..529)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZα fragment of β-galactosidase"
                     /transl_table=1
                     /translation="MTMITPSSELTLTKGNKSLHACRSTLEDPRVPSSNSPYSESYYNS
                     LAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFL
                     LTHLCGISHRIWCTLSTICSDAA*"
                     /label="lacZα"
                     /note="color: #993366"
     promoter        complement(2547..2651)
                     /gene="bla"
                     /label="AmpR promoter"
                     /note="color: #ffffff"
     misc_feature    423..479
                     /gene=""
                     /note="pUC18/19 multiple cloning site"
                     /note="color: #99ccff"
                     /label="MCS"
     promoter        complement(573..603)
                     /note="promoter for the E. coli lac operon"
                     /note="color: #ffffff"
                     /label="lac promoter"
     promoter        402..420
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff"
                     /label="T7 promoter"
     promoter        complement(486..504)
                     /note="promoter for bacteriophage T3 RNA polymerase"
                     /note="color: #ffffff"
                     /label="T3 promoter"
     primer_bind     379..395
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /note="color: #a020f0"
                     /label="M13 fwd"
     primer_bind     complement(525..541)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /note="color: #a020f0"
                     /label="M13 rev"
     protein_bind    549..565
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
                     /label="lac operator"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gtaatacgac tcactatagg
      421 gcgaattcga gctcggtacc cggggatcct ctagagtcga cctgcaggca tgcaagcttt
      481 tgttcccttt agtgagggtt aattccgagc ttggcgtaat catggtcata gctgtttcct
      541 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
      601 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
      661 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
      721 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
      781 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
      841 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
      901 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
      961 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     1021 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     1081 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     1141 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     1201 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     1261 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     1321 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     1381 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     1441 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     1501 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     1561 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     1621 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     1681 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     1741 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     1801 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     1861 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     1921 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     1981 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     2041 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     2101 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     2161 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     2221 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     2281 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     2341 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     2401 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     2461 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     2521 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     2581 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     2641 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     2701 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtc
//
